A5006019721- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Caveolin-1 and cellular processes
- PI3K/AKT/mTOR signaling in cancer
- Steroid Chemistry and Biochemistry
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Veterinary Medicine and Surgery
- Melanoma and MAPK Pathways
- Protease and Inhibitor Mechanisms
- Estrogen and related hormone effects
- Cardiovascular Conditions and Treatments
- MicroRNA in disease regulation
- Retinoids in leukemia and cellular processes
- Histone Deacetylase Inhibitors Research
- Endoplasmic Reticulum Stress and Disease
- Synthesis and biological activity
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Skin Protection and Aging
- Cancer Mechanisms and Therapy
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
Korea Institute of Radiological and Medical Sciences
2016-2025
Genomics (United Kingdom)
2023
Kangwon National University
2005-2021
Kyungpook National University
2020
Chungnam National University
2017
Sungkyunkwan University
2013
Dong-A University
2013
Kyushu Dental University
2002-2005
Zero to Three
2004
Chonnam National University
1998-2001
Abstract Glioblastoma is a severe type of primary brain tumor, and its highly invasive character considered to be major therapeutic obstacle. Several recent studies have reported that ionizing radiation (IR) enhances the invasion tumor cells, but mechanisms for this effect are not well understood. In study, we investigated possible signaling involved in IR-induced glioma cells. IR increased matrix metalloproteinase (MMP)-2 promoter activity, mRNA transcription, protein secretion along with...
Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage As2O3 cervical cancer and its possible mechanisms, human cell line HeLa was employed. The cells underwent apoptosis response to As2O3, accompanied by a decrease mitochondrial membrane caspase-3 activation. Overexpression Bcl-2, however, prevented dissipation potential, subsequently protecting from...
To prevent the development of malignancies, mammalian cells activate disposal programs, such as programmed cell death, in response to deregulated oncogene expression. However, molecular basis for regulation cellular machinery activated oncogenes is unclear at present. In this study, we show that upregulation autophagy-related protein, Atg5, critically required oncogenic H-ras-induced autophagic death and Rac1/mitogen-activated kinase (MKK) 7/c-Jun N-terminal (JNK) signals Atg5....
Ionizing radiation (IR) is widely used in cancer treatment and biological studies. It disrupts cellular homeostasis through multiple mechanisms including changes of the expression profile genes. Although microRNAs (miRNAs) have recently been recognized as important post-transcriptional regulators are involved various processes, whether miRNAs play any roles response to IR, not well examined. We investigated miRNA following IR human lung carcinoma cell line A549, profiles IR-responsive were...
Abstract Abnormal cell-cycle control can lead to aberrant cell proliferation and cancer. The oncoprotein cancerous inhibitor of protein phosphatase 2A (CIP2A) is an (PP2A) that stabilizes c-Myc. However, the precise role CIP2A in division not understood. Herein, we show required for mitotic progression by regulating polo-like kinase (Plk1). With entry, translocated from cytoplasm nucleus, where it was enriched at spindle poles. depletion delayed progression, resulting abnormalities...
Big data analysis has revealed the upregulation of cell division cycle associated 8 (CDCA8) in human hepatocellular carcinoma (HCC) and its poorer survival outcome. However, functions CDCA8 during HCC development remain unknown. Here, we demonstrate vitro that silencing inhibits growth long-term colony formation migration through accumulation G2/M phase population. Conversely, overexpression increases ability to undergo migration. RNA sequencing bioinformatic knockdown led same directional...
To elucidate possible mechanisms of anti-angiogenic activity by curcumin, we performed cDNA microarray and found that curcumin modulated cell cycle related gene expression. For further confirmation, DNA contents expression levels cyclins, cyclin-dependent kinases (CDKs), CDK inhibitors (CDKIs) were examined FACS analysis Western blotting, respectively. Curcumin was to induce G0/G1 and/or G2/M phase arrest, up-regulate CDKIs, p21WAF1/CIP1, p27KIP1, p53, slightly down-regulate cyclin B1 cdc2...
Abstract The net balance of matrix metalloproteinases (MMP) and tissue inhibitor (TIMP) system has been known to be a key factor in tumor cell invasion. In the present study, we investigated molecular mechanisms anti-invasive antimigrative activity transforming growth (TGF)-β1 on HT1080 human fibrosarcoma cells. vitro Matrigel invasion Transwell migration assays, TGF-β1 dose-dependently inhibited cells, respectively. Gelatin zymography, Western blot, real-time PCR analysis showed that...
Nerve growth factor (NGF) is a well characterized neurotrophic agonist in the nervous system that triggers angiogenesis. In this study, we investigated signaling mechanisms involved NGF-induced NGF stimulated endothelial cell invasion and cord formation on Matrigel vitro but had marginal effect proliferation migration of these cells. matrix metalloproteinase (MMP)-2 mRNA expression protein secretion human umbilical vein Using synthetic endogenous inhibitors MMP-2 small interfering RNA...
Abstract Amino acid availability is sensed by various signaling molecules, including general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). However, it unclear how these sensors are associated with cancer cell survival under low amino availability. In the present study, we investigated AKT activation in non-small lung (NSCLC) cells deprived each one 20 acids. Among acids, deprivation glutamine, arginine, methionine, lysine induced activation. was...
Ovarian cancer is a carcinoma that affects women and has high mortality rate. Overcoming paclitaxel resistance important for clinical application. However, the effect of amino acid metabolism regulation on paclitaxel-resistant ovarian still unknown. In this study, an acid-deprived condition in SKOV3-TR cells was analyzed. We analyzed cell viability culture conditions which each 20 acids were deprived. As result, significantly reduced cultures deprived arginine, glutamine, lysine....
Glioblastoma is one of the most radioresistant tumors. Exposure cells to ionizing radiation leads formation reactive oxygen species (ROS) that are associated with radiation-induced cytotoxicity. ROS scavengers, therefore, important factors in protecting against injury during exposure. In present study, we isolated and established a variant clone (RRC) from U251 human glioblastoma cell line investigated potential role antioxidant enzymes radioresistance line. RRC showed higher than parent as...
Recent studies have suggested that Skp2, an SCF-type ubiquitin ligase, positively regulates cell cycle through degradation of p27, which is inhibitor cyclin-dependent kinase 2 (CDK2), drives cells from the G1 to S phase cycles. In present study, we examined key regulatory proteins involved in serum starvation-induced arrest human ovarian cancer cells, SK-OV-3. Cell analysis showed were arrested at after starvation. Western blot protein levels CDK4 and CDK2 significantly decreased SK-OV-3...
Ionizing radiation (IR) disrupts cellular homeostasis through multiple mechanisms including changes of the expression profile genes. Although microRNAs (miRNAs), small single-stranded RNAs, have recently been recognized as important post-transcriptional regulators gene expression, it is not well investigated if miRNAs function in response to radiation. Therefore, we determined IR induces profiles and used this approach identify IR-responsive miRNAs. To monitor miRNAs, microarray analysis was...
Abstract Several groups have reported that TGFβ1 regulates cellular responses to γ-irradiation; however, the exact mechanism has not been fully elucidated. In current study, role of in γ-irradiation was investigated detail. The data indicate pretreatment decreased aftermath ionizing radiation (IR)-induced DNA damage a SMAD-dependent manner. To determine underlying for these effects, extent IR-induced repair activity presence or absence examined. Studies reveal upregulated ligase IV (Lig4),...
Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase inhibitor (HDACI) with antitumor effects that being explored as therapeutic drug. However, it has been reported non-small cell lung cancer (NSCLC) resistant to HDACIs. MicroRNAs (miRNAs) are key class of small, non-coding RNA molecules modulate post-transcriptional regulation gene expression in multi-cellular organisms. miRNA patterns involved deregulation human cancer. Here we identified profile changes response SAHA treatment...
microRNAs (miRNAs) have been shown to function as primary regulators of a variety biological processes, including proliferation, differentiation and apoptosis in human keratinocytes. However, the significance miRNAs defense against oxidative stress keratinocytes remains be elucidated. In this study, we demonstrate that oridonin, diterpenoid compound isolated from Rabdosia rubescens with established antioxidant properties, protects HaCaT induced by exposure hydrogen peroxide (H2O2). Our data...
Abstract Emerging evidence suggests that the mammalian target of rapamcyin (mTOR) pathway is associated with radio-resistance in cancer treatment. We hypothesised phosphorylated ribosomal S6 kinase 1 (p-S6K1), a major downstream regulator mTOR pathway, may play role predicting radio-resistance. Therefore, we evaluated association p-S6K1 expression breast cell lines and patients. During median follow-up 33 (range, 0.1–111) months for 1770 primary patients who underwent surgery, expressing...