Abstract The tumor suppressor p53 binds prosurvival Bcl-2 family proteins such as Bcl-w and Bcl-XL to liberate Bax, which in turn exerts proapoptotic or anti-invasive functions depending on stress context. On the basis of our previous finding that interacts with p21, we investigated possible involvement p21 these functions. Here, report although can bind alone, it requires Bax suppress cell invasion promote death. bound Bcl-w, forming a p53/p21/Bcl-w complex manner maintained all pairwise...

Autophagy has recently been implicated in both the prevention and progression of cancer. However, molecular basis for relationship between autophagy induction initial acquisition malignancy is currently unknown. Here, we provide first evidence that essential oncogenic K-Ras (K-Ras(V12))-induced malignant cell transformation. Retroviral expression K-Ras(V12) induced autophagic vacuole formation transformation human breast epithelial cells. Interestingly, pharmacological inhibition completely...

Nitric oxide (NO) during primary culture of articular chondrocytes causes apoptosis via p38 mitogen-activated protein kinase in association with elevation p53 level, caspase-3 activation, and differentiation status. In this study, we characterized the molecular mechanism by which induces through activation p53. We report here that NO-induced leads to NFkappaB, turn transcription gene. Activated also physically associates phosphorylates serine 15 residue p53, results accumulation apoptosis....

beta-Catenin regulates important biological processes, including embryonic development and tumorigenesis. We have investigated the role of beta-catenin in regulation chondrocyte phenotype. Expression was high prechondrogenic mesenchymal cells, but significantly decreased differentiated chondrocytes both vivo vitro. Accumulation by inhibition glycogen synthase kinase-3beta with LiCl inhibited chondrogenesis stabilizing cell-cell adhesion. Conversely, low level articular increased...

The prognosis of breast cancer patients is related to the degree metastasis. However, mechanisms by which epithelial tumor cells escape from primary and colonize at a distant site are not entirely understood. Here, we analyzed expression levels pituitary tumor-transforming gene-1 (PTTG1), relatively uncharacterized oncoprotein, in patient-derived tissues with corresponding normal tissues. We found that PTTG1 highly expressed patients, compared Also, were correlated malignancy cell lines;...

Bmal1 is a core factor in the regulation of circadian rhythms. Previous studies have shown that suppresses tumor growth cell culture and animal models downregulated certain types cancer. The aim present study was to investigated whether influences invasiveness cancer cells. We demonstrated knockdown by RNA interference promoted invasion, whereas its overexpression reduced cellular invasiveness. These effects were observed lung glioma cells, occurred regardless p53 status. Therefore, it...

We previously reported that podophyllotoxin acetate (PA) radiosensitizes NCI-H460 cells. Here, we confirmed PA treatment also induces cell death among two other non-small lung cancer (NSCLC) lines: NCI-H1299 and A549 cells (IC50 values = 7.6 16.1 nM, respectively). Our experiments further showed was able to induce via various mechanisms. First, dose-dependently induced cycle arrest at G2/M phase, as shown by accumulation of the mitosis-related proteins, p21, survivin Aurora B. This phase due...

Abstract Abnormal cell-cycle control can lead to aberrant cell proliferation and cancer. The oncoprotein cancerous inhibitor of protein phosphatase 2A (CIP2A) is an (PP2A) that stabilizes c-Myc. However, the precise role CIP2A in division not understood. Herein, we show required for mitotic progression by regulating polo-like kinase (Plk1). With entry, translocated from cytoplasm nucleus, where it was enriched at spindle poles. depletion delayed progression, resulting abnormalities...

Although radiotherapy resistance is associated with locoregional recurrence and distant metastasis in breast cancers, clinically relevant molecular markers critical signaling pathways of radioresistant cancer are yet to be defined. Herein, we show that HER2-STAT3-survivin regulation HER2-positive cancers. Depletion HER2 by siRNA sensitized cells irradiation decreasing STAT3 activity survivin, a target gene, expression cells. Furthermore, inhibition activation or depletion survivin also...

Our previous study indicated that interleukin (IL)‐1β induces expression of several Wnt proteins in chondrocytes and causes chondrocyte dedifferentiation via the c‐Jun/activator protein‐1 (AP‐1) pathway. This examined whether Wnt‐3a c‐Jun/AP‐1 inhibited chondrogenesis mesenchymal cells by stabilizing cell–cell adhesion a manner independent β‐catenin transcriptional activity. also induced articular stimulating activity β‐catenin‐T cell‐factor/lymphoid‐enhancer‐factor (Tcf/Lef) complex. In...

Accumulation of beta-catenin and subsequent stimulation beta-catenin-T cell-factor (Tcf)/lymphoid-enhancerfactor (Lef) transcriptional activity causes dedifferentiation articular chondrocytes, which is characterized by decreased type II collagen expression initiation I expression. This study examined the mechanisms alpha-catenin degradation, role in signaling, physiological significance regulation signaling chondrocytes. We found that both alpha- accumulated during chondrocytes escaping from...

Abstract Peroxiredoxins (PRDX) are a family of thiol-dependent peroxidases. Among the six mammalian members this family, PRDX6 is only protein that additionally exhibits phospholipase A2 (PLA2) activity. The physiologic role interesting feature largely unknown at present. In study, we show increases metastatic potential lung cancer cells. Functional analyses enzymatic activities PRDX6, using specific pharmacologic inhibitors and mutagenesis studies, reveal both peroxidase PLA2 required for...

Previous reports suggest that, in addition to its therapeutic effects, ionizing radiation (IR) increases the invasiveness of surviving cancer cells. Here, we demonstrate that this activity IR lung cells is mediated by a signaling pathway involving p38 kinase, phosphoinositide 3-kinase, Akt, and matrix metalloproteinase (MMP-2). The invasion-promoting doses also increased reduced levels vimentin E-cadherin, respectively, both which are markers for epithelial-mesenchymal transition (EMT)....

Phylogenetic analysis was conducted on 9 kDa non-specific lipid transfer protein (nsLTP) genes from nine plant species. Each of the five classified types in angiosperms exhibited eight conserved cysteine patterns. The most abundant nsLTP fell into type I category, which particularly enriched a grass-specific lineage clade I.1. Six pairs tandem copies distal region rice chromosomes 11 and 12 were well-preserved under concerted evolution, not observed sorghum. transgenic promoter-reporter...

// Min Ho Choe 1 , Joong Won Hong Bae Jeon 2 Dong-Hyung Cho 3 Jeong Su Oh 4 Hyun Gyu Lee 5 Sang-Gu Hwang Sungkwan An 6 Young-Hoon Han and Jae-Sung Kim Division of Radiation Cancer Research, Korea Institute Radiological Medical Sciences, Seoul, Biomedical Research Institute, MEDIPOST Co., Ltd., Graduate School East-West Science, Kyung Hee University, Suwon, Department Genetic Engineering, Sungkyunkwan Microbiology Immunology, College Medicine, Yonsei Molecular-Targeted Drug Center for Skin...

Glioblastoma, the most common primary brain tumor in adults, is an incurable malignancy with poor short-term survival and typically treated radiotherapy along temozolomide. While development of tumor-treating fields (TTFields), electric alternating low intermediate intensity has facilitated glioblastoma treatment, clinical outcomes TTFields are reportedly inconsistent. However, combinatorial administration chemotherapy proven effective for patients. Sorafenib, anti-proliferative apoptogenic...

Nitric oxide (NO) in articular chondrocytes regulates differentiation, survival, and inflammatory responses by modulating ERK-1 -2, p38 kinase, protein kinase C (PKC) α ζ. In this study, we investigated the effects of actin cytoskeletal architecture on NO-induced dedifferentiation, apoptosis, cyclooxygenase (COX)-2 expression, prostaglandin E2 production chondrocytes, with a focus ERK-1/-2, PKC signaling. Disruption cytoskeleton cytochalasin D (CD) inhibited COX-2 cultured plastic or during...

Although much is known about interleukin (IL)-1β and its role as a key mediator of cartilage destruction in osteoarthritis, only limited information available on IL-1β signaling chondrocyte dedifferentiation. Here, we have characterized the molecular mechanisms leading to dedifferentiation primary cultured articular chondrocytes by treatment. or lipopolysaccharide, but not phorbol 12-myristate 13-acetate, retinoic acid, epidermal growth factor, induced nicotinamide phosphoribosyltransferase...

A large number of really interesting new gene (RING) E3 ligases contribute to the post-translational modification target proteins during plant responses environmental stresses.However, physical interactome RING in rice remains largely unknown.Here, we evaluated expression patterns 47 Oryza sativa finger protein (OsRFP) genes response abiotic stresses via semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and silico analysis.Subsequently, molecular dissection nine...

Abstract Background Γ-Ionizing radiation (IR) therapy is one of major therapeutic tools in cancer treatment. Nevertheless, γ-IR failed due to occurrence metastasis, which constitutes a significant obstacle The main aim this investigation was construct animal model present metastasis during radiotherapy mouse system vivo and establishes the molecular mechanisms involved. Materials methods C6L transfectant cell line expressing firefly luciferase (fLuc) treated with γ-IR, followed by...