A5081016797- Radiation Therapy and Dosimetry
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Plant Genetic and Mutation Studies
- RNA modifications and cancer
- Effects of Radiation Exposure
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Boron Compounds in Chemistry
- Glioma Diagnosis and Treatment
- Chemical Reactions and Isotopes
- Occupational and environmental lung diseases
- Hepatocellular Carcinoma Treatment and Prognosis
- Nuclear Physics and Applications
- Advanced biosensing and bioanalysis techniques
- Cancer Mechanisms and Therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Heat shock proteins research
National Institutes for Quantum Science and Technology
2016-2023
National Institute of Health Sciences
2013
Research Institute of Radio
2009
The combination of CD44 and CD24, or aldehyde dehydrogenase 1 (ALDH1) alone, is a widely used cancer stem cell marker in breast cancer. However, no conclusion has yet been reached as to which the best for characterizing stemness. Immunohistochemical evaluation using markers clearly less common clinically than basic experiments how expressions these relate patient outcomes remains controversial. To investigate whether combining might improve prediction outcomes, we immunohistochemically...
Glioblastoma, the most common primary brain tumor in adults, is an incurable malignancy with poor short-term survival and typically treated radiotherapy along temozolomide. While development of tumor-treating fields (TTFields), electric alternating low intermediate intensity has facilitated glioblastoma treatment, clinical outcomes TTFields are reportedly inconsistent. However, combinatorial administration chemotherapy proven effective for patients. Sorafenib, anti-proliferative apoptogenic...
// Sei Sai 1 , Toshifumi Wakai 2 Guillaume Vares 3 Shigeru Yamada 4 Takehiko Kamijo 5 Tadashi Kamada and Toshiyuki Shirai Medical Physics Research Program, Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Japan Division Digestive General Surgery, Niigata University Graduate School Dental Niigata, Radiation Risk Reduction Protection, Hospital Clinical Oncology, Saitama Cancer Center, Ina, Saitama, Correspondence to: Sai, email: Keywords : carbon-ion...
Although a relatively small proportion of all breast cancer (BC), triple negative (TN) BC is responsible for large deaths because its worse clinical outcome. To investigate whether carbon ion beam alone or in combination with cisplatin (CDDP) has beneficial effect compared to X-rays, we target stem-like cells (CSCs).Human CSCs sorted from MDA-MB-231 and MDA-MB-453 were treated X-ray irradiation CDDP, then colony, spheroid tumor formation assays, RT-PCR Array analysis, immunofluorescence...
Colorectal cancer (CRC) is a major cause of mortality that can be treated effectively with chemotherapy and radiotherapy, although resistance to these therapeutic modalities often occurs. Tumor-treating fields (TTFields) block tumor growth by selectively impairing cell division. In this study, we investigated the mechanism which 5-fluorouracil (5-FU) sensitizes cells TTFields. Human HCT116 SW480 CRC were 5-FU and/or TTFields, characterized in vitro terms viability, apoptosis through reactive...
// Eun Ho Kim 1 , Mi-Sook 2 Yoshiya Furusawa 3 Akiko Uzawa Soorim Han Won-Gyun Jung Sei Sai Division of Heavy Ion Clinical Research, Korea Institute Radiological and Medical Sciences, Gongneung-dong, Nowon-Gu, Seoul, South Department Radiation Oncology, Basic Sciences for Damages, National Institutes Quantum Science Technology, Chiba, Japan Correspondence to: Kim, email: mskim@kirams.re.kr Sai, sai.sei@qst.go.jp Keywords: metformin, carbon ion beam, radiosensitivity, hepatocellular carcinoma...
// Sei Sai 1 , Masao Suzuki Eun Ho Kim 2 Mitsuhiro Hayashi 3 Guillaume Vares 4 Naoyoshi Yamamoto 5 and Tadaaki Miyamoto 6 Department of Basic Medical Sciences for Radiation Damages, National Institute Radiological Sciences, Institutes Quantum Science Technology, Chiba, Japan Division Applied Bioscience, Korea Gongneung-dong, Nowon-Gu, Seoul, South Breast Center, Dokkyo University Hospital, Mibu-machi, Shimotsuga-gun, Tochigi, Okinawa Technology (OIST), Advanced Instrumentation Unit,...
The purpose of this study was to investigate the potential gold nanoparticles as radiosensitizer for use in neutron therapy against hepatocellular carcinoma. carcinoma cells lines Huh7 and HepG2 were irradiated with γ radiation presence or absence nanoparticles. Effects evaluated by transmission electron microscopy, cell survival, cycle, DNA damage, migration, invasiveness. Gold significantly enhanced radiosensitivity γ-rays 1.41- 1.16-fold, respectively, 1.80- 1.35-fold radiation, which has...
Osteosarcoma (OS) is a malignant tumor of the bone derived from primitive transformed cells mesenchymal origin. Local low-linear energy transfer (LET) radiotherapy has limited benefits on OS owing to its radioresistance. Thus, this study aimed investigate effects high-LET radiation human OS. Therefore, cell lines, U2O2 and KHOS/NP, were examined in vitro, or an orthotopic mouse xenograft model was studied vivo after treatment with low-LET (gamma-ray) (neutron) radiation. Notably,...
Following carbon ion beam irradiation in mammalian cells, such as used radiotherapy (CIRT), it has been suggested that the balance between whether nonhomologous end joining (NHEJ) or homologous recombination (HR) is utilized depends on DNA double-strand break (DSB) complexity. Here, we quantified DSB distribution and identified importance of each repair pathway at increasing depths within spread-out Bragg peak (SOBP) range. Chinese hamster ovary (CHO) cell lines were irradiated a single...
Osteosarcoma (OSA) is the most common malignant bone tumor in children and adolescents. The overall five-year survival rate for all cancers below 70%; however, when cancer has spread beyond bone, it about 15-30%. Herein, we evaluated effects of carbon-ion beam irradiation alone or combination with zoledronic acid (ZOL) on OSA cells. Carbon-ion ZOL significantly inhibited cell proliferation by arresting cycle progression initiating KHOS U2OS apoptosis, compared to treatments irradiation,...
Osteosarcoma (OS) originates from osteoid bone tissues and is prone to metastasis, resulting in a high mortality rate. Although several treatments are available for OS, an effective cure does not exist most patients with advanced OS. Zoledronic acid (ZOL) third-generation bisphosphonate that inhibits osteoclast-mediated resorption has shown efficacy treating metastases various types of solid tumors. Here, we sought clarify the mechanisms through which ZOL OS cell proliferation. treatment...
The study investigated the molecular mechanisms that killed pancreatic cancer cells, including stem cells (CSCs), by carbon ion beam irradiation alone or in combination with miRNA-200c under vitro and vivo conditions.Human (PC) PANC1 PK45, were treated carbon-ion microRNA-200c (miR-200c) mimic. Cell viability assay, colony spheroid formation quantitative real-time PCR analysis of apoptosis-, autophagy-, angiogenesis-related gene expression, xenograft tumor control histopathological analyses...
Radiotherapy using high linear energy transfer (LET) radiation results in effectively killing tumor cells while minimizing dose (biological effective) to normal tissues block toxicity. It is well known that LET leads lower cell survival per absorbed than low radiation. High-linear neutron treatment induces autophagy cells, but its precise mechanisms osteosarcoma are unknown. Here, we investigated this mechanism and the underlying signaling pathways. Autophagy induction was examined...
Liver cancer is a common malignancy worldwide, with poor prognosis and high recurrence rate despite the available treatment methodologies. Tumor-treating fields (TTFields) have shown good preclinical clinical results for improving of patients glioblastoma malignant pleural mesothelioma. However, there minimal evidence effect TTFields on other types. Thus, present study aimed to investigate therapeutic efficacy in an vitro model, further elucidate underlying mechanisms. In study, two...
The aim of this study was to investigate whether carbon-ion beam irradiation in combination with 5-fluorouracil (5-FU) is superior alone targeting colorectal cancer stem-like cells (CSCs).Human (CRC) cells, HCT116 and HT29, were treated or 5-FU. Cell viability assay, colony spheroid formation apoptotic quantitative real-time PCR analysis apoptosis- autophagy-related gene expression performed.Carbon-ion dose-dependently decreased CRC cell showed significantly enhanced killing effect when...
Abstract Among the four main subtypes of breast cancer, basal-like cancer (the predominant subtype triple-negative cancer) is particular concern, due to its high frequency, relative lack effective therapies and poor prognosis. Ionizing radiation (for example as treatment for other cancers) cumulative exposure steroid hormones (as seen in postmenopausal women under hormone replacement therapy) are known risk factors cancer. Because their tumor-initiating properties, stem cells (CSCs) were...