Piscirickettsia salmonis is one of the main infectious diseases affecting coho salmon (Oncorhynchus kisutch) farming, and current treatments have been ineffective for control this disease. Genetic improvement P. resistance has proposed as a feasible alternative disease in farmed fish. Genotyping by sequencing (GBS) strategies allow genotyping hundreds individuals with thousands single nucleotide polymorphisms (SNPs), which can be used to perform genome wide association studies (GWAS) predict...

The estimation of linkage disequilibrium between molecular markers within a population is critical when establishing the minimum number required for association studies, genomic selection, and inferring historical events influencing different populations. This work aimed to evaluate extent decay in coho salmon breeding using high-density SNP array. Linkage was estimated total 93,502 SNPs found 64 individuals (33 dams 31 sires) from population. encompass all 30 chromosomes comprise 1,684.62...

Nile tilapia (Oreochromis niloticus) is one of the most cultivated and economically important species in world aquaculture. Intensive production promotes use monosex animals, due to an dimorphism that favors male growth. Currently, main mechanism obtain all-male populations hormones feeding during larval fry phases. Identifying genomic regions associated with sex determination a research topic great interest. The objective this study was identify variants three commercial tilapia....

Nile tilapia (Oreochromis niloticus) is one of the most produced farmed fish in world and represents an important source protein for human consumption. Farmed populations are increasingly based on genetically improved stocks, which have been established from admixed populations. To date, there scarce information about population genomics tilapia, assessed by dense single nucleotide polymorphism (SNP) panels. The patterns linkage disequilibrium (LD) may affect success genome-wide association...

Infectious pancreatic necrosis (IPN) is a viral disease with considerable negative impact on the rainbow trout (Oncorhynchus mykiss) aquaculture industry. The aim of present work was to detect genomic regions that explain resistance infectious virus (IPNV) in trout. A total 2,278 fish from 58 full-sib families were challenged IPNV and 768 individuals genotyped (488 resistant 280 susceptible), using 57K SNP panel Axiom, Affymetrix. genome-wide association study (GWAS) performed phenotypes...

Using a λgt10 cDNA library obtained from Xenopus laevis oocytes and probes derived the known sequences of human Drosophila genes, coding for α‐subunit X. casein kinase II was isolated. The sequence this clone determines polypeptide 350 amino acids. is 98% identical to rat proteins in first 323 polymerase chain reaction generate 370‐nucleotide‐long probe, it possible 900 nucleotides that coded β‐subunit II. protein 215 acids long again shows an extraordinary degree conservation with other species.

Abstract CK1 constitutes a protein kinase subfamily that is involved in many important physiological processes. However, there limited knowledge about mechanisms regulate their activity. Isoforms CK1δ and CK1ε were previously shown to autophosphorylate carboxy‐terminal sites, process which effectively inhibits catalytic Mass spectrometry of CK1α splice variant CK1αL has identified the autophosphorylation last four carboxyl‐end serines threonines also for CK1αS, same residues plus...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTActivity of recombinant .alpha. and .beta. subunits casein kinase II from Xenopus laevisMaria V. Hinrichs, Ana Jedlicki, Rowena Tellez, Sandor Pongor, Marta Gatica, Catherine C. Allende, Jorge E. AllendeCite this: Biochemistry 1993, 32, 28, 7310–7316Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July...

CaSein kinase II purified from the nuclei of Xenopus laevis oocytes as well recombinant α and β subunits X. CKII, produced in E. coli cloned cDNA genes, were tested with different divalent metal ions. The enzyme both sources was active either Mg 2+ , Mn or Co . Optimal concentrations 7–10 mM for 0.5–0.7 1–2 In presence used GTP more efficiently than ATP a phosphate donor while reverse true ofMg apparent K m values nucleotide triphosphates greatly decreased compared Addition Zn (above 150 μM)...

On the basis of morphological evidence, species involved in South American Pacific coast harmful algal blooms (HABs) has been traditionally recognized as Alexandrium catenella (Dinophyceae). However, these observations have not confirmed using evidence based on genomic sequence variability. Our principal objective was to accurately determine local HABs order implement a real-time polymerase chain reaction (PCR) assay for its rapid and easy detection filter-feeding shellfish, such mussels.For...

Abstract Hamster spermatozoa are able to fertilize a high percentage of zona‐intact hamster oocytes when they preincubated for 2 hr in chemically defined medium. From this time on, the longer preincubation lower penetration. Spermatozoa 6 or more unable cross zona pellucida, retaining however their ability fuse with zona‐free oocytes. Zona‐intact oocytes, as described above, were observed scanning electron microscope. When inseminated 1 5 outer surface showed penetrating and sperm tracks...

Summary Relatively large rates of response to traits economic importance have been observed in different selection experiments salmon. Several QTL mapped the salmon genome, explaining unprecedented levels phenotypic variation. Owing relatively intensity, individual loci may be indirectly selected, leaving molecular footprints selection, together with increased inbreeding, as its likely relatives will share selected loci. We used population differentiation and linkage disequilibrium...

Protein kinase CK1, also known as casein 1, participates in the phosphorylation of β-catenin, which regulates functioning Wnt signaling cascade involved embryogenesis and carcinogenesis. β-catenin occurs a multiprotein complex assembled on scaffold protein axin. The interaction CK1α from Danio rerio with mouse-axin has been studied using pull-down assay that uses fragments axin fused to glutathione S transferase, is bound sepharose beads. results indicate three lysines present basic region...

The cDNA gene coding for the α subunit of Xenopus laevis casein kinase II was mutated using overlap extension PCR method. mutation substituted glutamic acids Lys 75 and 76 , changing charge distribution a very basic sequence found in subunit. Expression pT7‐7 vector E. coli yielded an active mutant recombinant protein that extensively purified. This not significantly affected its app. K m or model peptide substrate, nor interaction with activating β Inhibition by quercetin 5,6‐dichloro‐1‐β‐...

The value of the gamete fusion test for assessment human spermatozoa is assessed. factors influencing are discussed, including conditions heterologous fertilization using zona-free oocytes such as nature sperm preparation, concentration and capacitation time, importance albumin in medium. can be examined before or after fixation, around eggs assessed acrosome changes, while number tails on oocyte related to chromatin decondensations. provides information fertilizing ability given...

The catalytic subunit of protein kinase casein 2 (CK2α), which has specificity for both ATP and GTP, shows significant amino acid sequence similarity to the cyclin-dependent (CDK2). We constructed site-directed mutants CK2α used a three-dimensional model investigate basis dual specificity. Introduction Phe Gly at positions 50 51, in order restore pattern glycine-rich motif, did not seriously affect or GTP. show that probably originates from loop situated around position His115 Asp120...

Abstract Aged spontaneously activated hamster oocytes recovered from adult females 18 and 24 hours after ovulation were at the pronuclear stage. These fresh controls inseminated in vitro with capacitated spermatozoa observed phase‐contrast microscope. The percentage of fertilization control was 98%, as compared to 36% 18% when ovulation, respectively. mean number sperm decondensations per egg 10, aged ones it 0.69 0.12 When similarly treated studied scanning transmission electron microscopy,...

ABSTRACT Piscirickettsia salmonis is one of the main infectious diseases affecting coho salmon ( Oncorhynchus kisutch ) farming. Current treatments have been ineffective for control disease. Genetic improvement P. resistance has proposed as a feasible alternative this disease in farmed fish. Genotyping by sequencing (GBS) strategies allow genotyping hundreds individuals with thousands single nucleotide polymorphisms (SNPs), which can be used to perform genome wide association studies (GWAS)...

Abstract The ultrastructure of human cervical mucus was studied by scanning electron microscopy. In fertile women, naturally occurring midcycle showed an arrangement parallel fibers oriented along the main axis sample. Sperm migration through a column in vitro also studied. It found that sperm present among longitudinal were to them and

Abstract The estimation of linkage disequilibrium between molecular markers within a population is critical when establishing the minimum number required for association studies, genomic selection and inferring historical events influencing different populations. This work aimed to evaluate extent decay in coho salmon breeding using ddRAD markers. Linkage was estimated total 7,505 SNPs found 62 individuals (33 dams 29 sires) from population. makers encompass all 30 chromosomes comprise...

Abstract Hamster and mouse capacitated spermatozoa were interacted in vitro with hamster eggs homologous heterologous combinations. Also, fertilized trypsin treated unfertilized eggs, rat made to interact spermatozoa. The surface of the zona pellucida was then examined scanning electron microscope. It found that sperm attachment, followed by binding penetration through pellucida, observed only when gamete combinations used. Binding evidenced lytic effect acrosomal enzymes on substance. When...

ABSTRACT Nile Tilapia ( Oreochromis niloticus ) is the second most important farmed fish in world and a sustainable source of protein for human consumption. Several genetic improvement programs have been established this species so far, they are mainly based on conventional selection using genealogical phenotypic information to estimate merit breeders make decisions. Genome-wide can be exploited efficiently incorporate traits that difficult measure breeding goal. Thus, SNPs required...

Abstract Nile tilapia ( Oreochromis niloticus ) is one of the most produced farmed fish in world and represents an important source protein for human consumption. Farmed populations are increasingly based on genetically improved stocks, which have been established from admixed populations. To date, there scarce information about population genomics tilapia, assessed by dense single nucleotide polymorphism (SNP) panels. The patterns linkage disequilibrium (LD) may affect success genome-wide...