A5047084538- Autophagy in Disease and Therapy
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Genetic and Kidney Cyst Diseases
- Endoplasmic Reticulum Stress and Disease
- Oral Health Pathology and Treatment
- Sirtuins and Resveratrol in Medicine
- Prion Diseases and Protein Misfolding
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Antifungal resistance and susceptibility
- Cannabis and Cannabinoid Research
- Calpain Protease Function and Regulation
- Genetic Neurodegenerative Diseases
- Oral and gingival health research
- Parkinson's Disease Mechanisms and Treatments
- Tissue Engineering and Regenerative Medicine
- Liver physiology and pathology
- Calcium signaling and nucleotide metabolism
- Ocular Infections and Treatments
- Liver Disease Diagnosis and Treatment
- biodegradable polymer synthesis and properties
- Regulation of Appetite and Obesity
- Lipid metabolism and biosynthesis
- Genetics and Neurodevelopmental Disorders
University of Chile
2007-2024
Montavid Thermodynamic Research Group
2019-2024
Advanced Center for Chronic Diseases
2017
International Centre for Genetic Engineering and Biotechnology
2011-2014
Weatherford College
2014
TDP-43 aggregates are the neurohistological landmark of diseases like amyotrophic lateral sclerosis and frontotemporal dementia. Their role in pathogenesis these conditions is not yet clear mainly due to lack proper models aggregation that may allow study mechanism formation, their interactions with other cellular components effect on cell metabolism. In this work, we have used tandem repeats prion Q/N-rich region TAR DNA-binding protein (TDP-43) fused additional sequences trigger aggregate...
TAR DNA binding protein 43 kDa (TDP-43) is a ribonuclear regulating many aspects of RNA metabolism. Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD) are fatal neurodegenerative diseases with the presence TDP-43 aggregates in neuronal cells. Chaperone Mediated Autophagy (CMA) lysosomal degradation pathway participating proteostasis several cytosolic proteins including associated proteins. In addition, oligomers or can affect status CMA. this work, we studied...
Abstract Palmitic acid (PA) is significantly increased in the hypothalamus of mice, when fed chronically with a high-fat diet (HFD). PA impairs insulin signaling hypothalamic neurons, by mechanism dependent on autophagy, process lysosomal-mediated degradation cytoplasmic material. In addition, previous work shows crosstalk between autophagy and primary cilium (hereafter cilium), an antenna-like structure cell surface that acts as platform for cell. Ciliopathies, human diseases characterized...
// Daniel Peña-Oyarzun 1,2,* , Rodrigo Troncoso 1,3,* Catalina Kretschmar 1,4 Cecilia Hernando Mauricio Budini 4 Eugenia Morselli 5 Sergio Lavandero 1,2,6 and Alfredo Criollo 1 Advanced Center for Chronic Diseases, Facultad Ciencias Quimicas y Farmaceuticas & Medicina, Universidad de Chile, Santiago, Chile 2 Molecular Studies of the Cell, 3 Instituto Nutrición Tecnología los Alimentos, Investigación en Odontológicas, Odontología, Departamento Fisiología, Biológicas, Pontificia Católica 6...
Abstract Ferroptosis is implicated in the pathogenesis of numerous chronic-inflammatory diseases, yet its association with progressive periodontitis remains unexplored. To investigate involvement and significance ferroptosis progression, we assessed sixteen periodontitis-diagnosed patients. Disease progression was clinically monitored over twelve weeks via weekly clinical evaluations gingival crevicular fluid (GCF) collection performed for further analyses. Clinical metrics, proteomic data,...
Recurrence and resistance of Candida spp. infections is associated with the ability these microorganisms to present several virulence patterns such as morphogenesis, adhesion, biofilm formation. In search for agents antivirulence activity, essential oils could represent a strategy act against biofilms potentiate antifungal drugs.To evaluate effect Origanum vulgare L. oil (O-EO) fluconazole nystatin.The O-EO was evaluated on ATCC reference strains C. albicans non-albicans species. Minimum...
The rotavirus (RV) non-structural protein 5, NSP5, is encoded by the smallest of 11 genomic segments and localizes in 'viroplasms', cytoplasmic inclusion bodies which viral RNA replication packaging take place. NSP5 essential for replicative cycle virus because, its absence, viroplasms are not formed transcription do occur. produced early infection undergoes a complex hyperphosphorylation process, leading to formation proteins differing electrophoretic mobility. role unknown. Previous vitro...
TDP-43 inclusions are an important histopathological feature in various neurodegenerative disorders, including Amyotrophic Lateral Sclerosis and Fronto-Temporal Lobar Degeneration. However, the relation of these with pathogenesis disease is still unclear. In fact, could be toxic themselves, induce loss function by sequestering or a combination both. Previously, we have developed cellular model aggregation using Q/N rich amino acid sequence 331-369 repeated 12 times (12xQ/N) shown that...
Drug-induced liver injury (DILI) is one of the leading causes acute injury. While many factors may contribute to susceptibility DILI, obese patients with hepatic steatosis are particularly prone suffer DILI. The secretome derived from mesenchymal stem cell has been shown have hepatoprotective effects in diverse vitro and vivo models. In this study, we evaluate whether MSC could improve DILI mediated by amiodarone (AMI) or tamoxifen (TMX). Hepatic HepG2 HepaRG cells were incubated AMI TMX,...
Abstract Background and Objective There is no clear understanding of molecular events occurring in the periodontal microenvironment during clinical disease progression. Our aim was to explore qualitative quantitative differences gingival crevicular fluid (GCF) protein profiles from patients diagnosed with periodontitis between non‐progressive progressive sites. Methods Five systemically healthy were monitored weekly their progression GCF samples 10 candidate sites obtained. Two groups five...
Abstract CK1 constitutes a protein kinase subfamily that is involved in many important physiological processes. However, there limited knowledge about mechanisms regulate their activity. Isoforms CK1δ and CK1ε were previously shown to autophosphorylate carboxy‐terminal sites, process which effectively inhibits catalytic Mass spectrometry of CK1α splice variant CK1αL has identified the autophosphorylation last four carboxyl‐end serines threonines also for CK1αS, same residues plus...
Obesity represents a significant global health challenge characterized by chronic low-grade inflammation and metabolic dysregulation. The hypothalamus, key regulator of energy homeostasis, is particularly susceptible to obesity's deleterious effects. This study investigated the role immunoproteasome, specialized proteasomal complex implicated in cellular during diseases.
Muscle atrophy involves a massive catabolism of intracellular components leading to significant reduction in cellular and tissue volume. In this regard, autophagy, an mechanism that degrades proteins organelles, has been implicated with muscle breakdown. Recently, it shown polycystin-2 (PC2), membrane protein belongs the transient receptor potential (TRP) family, is required for maintenance proteostasis, by regulating autophagy several cell types. The role PC2 control skeletal remains...
The intake of food with high levels saturated fatty acids (SatFAs) is associated the development obesity and insulin resistance. SatFAs, such as palmitic (PA) stearic (SA) acids, have been shown to accumulate in hypothalamus, causing several pathological consequences. Autophagy a lysosomal-degrading pathway that can be divided into macroautophagy, microautophagy, chaperone-mediated autophagy (CMA). Previous studies showed PA impairs macroautophagy function response hypothalamic...