A5085850265- RNA Research and Splicing
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Signaling Pathways in Disease
- Protein Degradation and Inhibitors
- vaccines and immunoinformatics approaches
- Multiple Myeloma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- RNA Interference and Gene Delivery
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- DNA Repair Mechanisms
- Pregnancy and preeclampsia studies
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
Cancer Institute of New South Wales
2019-2022
UNSW Sydney
2017-2022
Prince of Wales Hospital
2021
Technical University of Denmark
2019
Copenhagen University Hospital
2019
Abstract Human endogenous retroviruses (HERV) form a substantial part of the human genome, but mostly remain transcriptionally silent under strict epigenetic regulation, yet can potentially be reactivated by malignant transformation or therapies. Here, we evaluate potential for T cell recognition HERV elements in myeloid malignancies mapping transcribed genes and generating library 1169 antigenic HERV-derived peptides predicted presentation 4 HLA class I molecules. Using DNA barcode-labeled...
Abstract Chemo-resistance in acute myeloid leukemia (AML) patients is driven by leukemic stem cells (LSCs) resulting high rates of relapse and low overall survival. Here, we demonstrate that upregulation the splicing factor, RBM17 preferentially marks sustains LSCs directly correlates with shorten patient knockdown primary AML leads to differentiation impaired colony formation vivo engraftment. Integrative multi-omics analyses show repression inclusion poison exons production...
High-constitutive activity of the DNA damage response protein checkpoint kinase 1 (CHK1) has been shown in glioblastoma (GBM) cell lines and tissue sections. However, whether constitutive activation overexpression CHK1 GBM plays a functional role tumorigenesis or prognostic significance is not known. We interrogated multiple glioma patient cohorts for expression levels oncogene cancerous inhibitor phosphatase 2A (CIP2A), known target high-CHK1 activity, examined relationship between these...
Abstract The human transcriptome consists of various RNA biotypes including multiple types non-coding RNAs (ncRNAs). Current ncRNA compendia remain incomplete partially because they are almost exclusively derived from the interrogation small- and polyadenylated RNAs. Here, we present a more comprehensive atlas that is matching polyA-, total-, small-RNA profiles heterogeneous collection nearly 300 tissues cell lines. We report on thousands novel species across all major biotypes, hitherto...
RNA splicing is a fundamental biological process that generates protein diversity from finite set of genes. Recurrent somatic mutations factor genes are relatively uncommon in Acute Myeloid Leukemia (AML, < 20%). We examined whether differences exist AML even the absence mutations. Analyzing RNA-seq data two independent cohorts patients, we identified recurrent differential alternative between patients with poor and good prognosis. These events occurred without any discernible The...
<p>Supplementary Figure 4, related to main 5. Data pertaining independent validation in the BEAT-AML cohort.</p>
<p>Supplementary Figure 1, related to main 1</p>
<p>Supplementary Figure 1, related to main 1</p>
<p>Supplementary Figure 2, related to main 3</p>
<p>Supplementary Figure 3, related to main 5.</p>
<p>Supplementary Figure 2, related to main 3</p>
<p>Supplementary Figure 3, related to main 5.</p>
<p>Supplementary Figure 4, related to main 5. Data pertaining independent validation in the BEAT-AML cohort.</p>
<p>Supplementary Methods - revised, plus Supplementary Tables 1-6</p>
<div>AbstractPurpose:<p>RNA splicing is a fundamental biological process that generates protein diversity from finite set of genes. Recurrent somatic mutations factor genes are common in some hematologic cancers but relatively uncommon acute myeloid leukemia (AML, < 20% patients). We examined whether RNA differences exist AML, even the absence mutations.</p>Experimental Design:<p>We developed bioinformatics pipeline to study alternative RNA-sequencing data large...
<div>AbstractPurpose:<p>RNA splicing is a fundamental biological process that generates protein diversity from finite set of genes. Recurrent somatic mutations factor genes are common in some hematologic cancers but relatively uncommon acute myeloid leukemia (AML, < 20% patients). We examined whether RNA differences exist AML, even the absence mutations.</p>Experimental Design:<p>We developed bioinformatics pipeline to study alternative RNA-sequencing data large...
<p>Supplementary Methods - revised, plus Supplementary Tables 1-6</p>
<p>Figure S1, related to Figure 1: Expression and correlation of CHK1 CIP2A in multiple glioma cohorts. S2, Overall survival mRNA expression PP2A subunits patients. S3, Role CHK1-CIP2A Glioma Stem cell lines. S4, 3: Effect or on GBM cells. S5, 5: Depletion induces senescence S6, 6: Regulation by STAT3.</p>
<p>Figure S1, related to Figure 1: Expression and correlation of CHK1 CIP2A in multiple glioma cohorts. S2, Overall survival mRNA expression PP2A subunits patients. S3, Role CHK1-CIP2A Glioma Stem cell lines. S4, 3: Effect or on GBM cells. S5, 5: Depletion induces senescence S6, 6: Regulation by STAT3.</p>
<div>Abstract<p>High-constitutive activity of the DNA damage response protein checkpoint kinase 1 (CHK1) has been shown in glioblastoma (GBM) cell lines and tissue sections. However, whether constitutive activation overexpression CHK1 GBM plays a functional role tumorigenesis or prognostic significance is not known. We interrogated multiple glioma patient cohorts for expression levels oncogene cancerous inhibitor phosphatase 2A (CIP2A), known target high-CHK1 activity, examined...