A5049389962- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Genetic and Kidney Cyst Diseases
- Retinal Development and Disorders
- Insect Pheromone Research and Control
- Hedgehog Signaling Pathway Studies
- Insect and Pesticide Research
- Protist diversity and phylogeny
- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Advanced Chemical Sensor Technologies
- Antioxidant Activity and Oxidative Stress
- Photoreceptor and optogenetics research
- Developmental Biology and Gene Regulation
- Bee Products Chemical Analysis
- Genetic Syndromes and Imprinting
- Venomous Animal Envenomation and Studies
- Cerebrospinal fluid and hydrocephalus
- Neuropeptides and Animal Physiology
- Proteins in Food Systems
- Neuroscience and Neuropharmacology Research
- Visual perception and processing mechanisms
- Healthcare and Venom Research
- Cellular transport and secretion
University of Florida
2014-2024
Taste and Smell Clinic
2012-2023
Florida College
2017-2023
Whitney Museum of American Art
2007-2014
University of Maryland, Baltimore
2006
University of Potsdam
1998-2001
GTx (United States)
2000
Institute of Evolutionary Physiology and Biochemistry
1987-1996
University of Maryland, College Park
1995
In vertebrates, olfactory receptors localize on multiple cilia elaborated dendritic knobs of sensory neurons (OSNs). Although dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor traditionally linked with motile biogenesis, expressed OSNs required for epithelium (OE) formation. keeping immotile nature cilia, we...
The nature of the olfactory receptor in crustaceans, a major group arthropods, has remained elusive. We report that spiny lobsters, Panulirus argus, express ionotropic receptors (IRs), insect chemosensory variants glutamate receptors. Unlike insects IRs, which are expressed specific subset cells, two lobster IR subunits most, if not all, neurons (ORNs), as confirmed by antibody labeling and situ hybridization. Ligand-specific ORN responses visualized calcium imaging consistent with...
Phosphoinositide signaling, in particular, phosphoinositide 3-kinase (PI3K) has been implicated mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand this phenomenon we investigated PI3K-dependent inhibition between single pairs. The concentration-dependent of the response native rat ORNs octanol by citral is PI3K dependent; blocking activity with β and γ isoform-specific inhibitors AS252424...
Olfactory dysfunction is a pervasive but underappreciated health concern that affects personal safety and quality of life. Patients with olfactory dysfunctions have limited therapeutic options, particularly those involving congenital diseases. Bardet-Biedl syndrome (BBS) one such disorder, where loss other symptoms manifest from defective cilium morphology and/or function in various cell types/tissues. sensory neurons (OSNs) BBS mutant mice lack the capacity to build/maintain cilia,...
Cilia of olfactory sensory neurons (OSNs) are the primary site odor binding; hence, their loss results in anosmia, a clinical manifestation pleiotropic ciliopathies for which there no curative therapies. We used OSN-specific Ift88 knock-out mice (Ift88osnKO) both sexes to examine mechanisms ciliopathy-induced dysfunction and potential gene replacement rescue odorant detection, restore circuitry, odor-guided behaviors. Loss OSN cilia Ift88osnKO resulted substantially reduced detection...
Bardet-Beidl Syndrome (BBS) manifests from genetic mutations encoding for one or more BBS proteins. BBS4 loss impacts olfactory ciliation and odor detection yet the cellular mechanisms remain unclear. Here, we report that Bbs4−/− mice exhibit shorter fewer sensory neuron (OSN) cilia despite retaining odorant receptor localization. Within OSN cilia, observed asynchronous rates of IFT-A/B particle movements indicating miscoordination in IFT complex trafficking. dendritic knob, basal bodies are...
Odorants inhibit as well excite olfactory receptor neurons (ORNs) in many species of animals. Cyclic nucleotide-dependent activation canonical mammalian ORNs is established but it still unclear how odorants these cells. Here we further implicate phosphoinositide-3-kinase (PI3K), an indispensable element PI signaling cellular processes, transduction rodent ORNs. We show that rapidly and transiently activate PI3K the cilia epithelium vitro. known G-protein-coupled isoforms they modulate not...
Peptide hormones and their cognate receptors belonging to neuropeptide Y (NPY) family mediate diverse biological functions in a number of tissues. Recently, we discovered the presence gut satiation peptide YY (PYY) saliva mice humans defined its role regulation food intake body weight maintenance. Here report systematic analysis expression patterns all NPY (Rs), Y1R, Y2R, Y4R, Y5R lingual epithelia mice. Using four independent assays, immunohistochemistry, situ hybridization,...
The lipid composition of the primary cilia membrane is emerging as a critical regulator formation, maintenance and function. Here, we show that conditional deletion phosphoinositide 5'-phosphatase gene Inpp5e, mutation which causative Joubert syndrome, in terminally developed mouse olfactory sensory neurons (OSNs), leads to dramatic remodeling ciliary phospholipids accompanied by marked elongation cilia. Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2], normally restricted proximal...
Sensory neurons in the mouse vomeronasal organ consist of two major groups, apical and basal, that project to different brain regions, express unique sets receptors, serve distinct functions. Electrical properties these subpopulations, however, have not been systematically characterized. V1rb2-tau-GFP V2r1b-tau-GFP tagged sensory (VSNs) were selected as prototypical or basal VSNs, respectively, their biophysical analyzed acute slices minimized cell damage. Basal V2r1b-expressing VSNs had...
Several Ca-sensitive fluorescent dyes (fura-2, mag-fura-2 and Calcium Green-5N) were used to measure intracellular calcium ion concentration, Cai, accompanying light-induced excitation of Limulus ventral nerve photoreceptors. A ratiometric procedure was developed for quantification Green-5N fluorescence. mixture a Ca-insensitive dye, ANTS, injected in the cell fluorescence intensities both calculate spatial average Cai within light-sensitive R lobe photoreceptor. In dark-adapted...
Phosphoinositide 3-kinase (PI3K) signaling has been implicated in mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand the breadth of such inhibition odor coding, we screened a panel odorants representing different chemical classes, as well known occur natural object (tomato), for their ability rapidly activate PI3K-dependent signaling. Odorants were on dissociated native rat ORNs before and after pre-incubation with PI3K-isoform specific...
Bardet-Biedl syndrome (BBS) is a hereditary genetic disorder that results in numerous clinical manifestations including olfactory dysfunction. Of at least 21 BBS-related genes can carry multiple mutations, pathogenic mutation, BBS1M390R, the single most common mutation of clinically diagnosed BBS outcomes. While deletion mice cause variable penetrance different organ systems, impact Bbs1M390R system remains unclear. Using relevant knock-in mouse model homozygous for Bbs1M390R, we...
An emerging idea in olfaction is that temporal coding of odor specificity can be intrinsic to the primary olfactory receptor neurons (ORNs). As a first step towards understanding whether lobster ORNs are capable generating odor-specific activity and what mechanisms underlie any such heterogeneity discharge pattern, we characterized different patterns individually ensemble using patch-clamp recording calcium imaging. We demonstrate show tonic excitation, inhibition, phaso-tonic bursting,...
ABSTRACT Olfactory sensory neurons (OSNs) form embryonically and mature perinatally, innervating glomeruli extending dendrites with multiple cilia. This process its timing are crucial for odor detection perception continues throughout life. In the olfactory epithelium (OE), differentiated OSNs proceed from an immature (iOSN) to a (mOSN) state through well-defined sequential morphological molecular transitions, but precise mechanisms controlling OSN maturation remain largely unknown. We have...
ABSTRACT In vertebrates, olfactory receptors localize on multiple cilia elaborated dendritic knobs of sensory neurons (OSNs). Although dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor linked with motile biogenesis, expressed OSNs required for epithelium (OE) formation. keeping immotile nature cilia, we observed...
Olfactory GPCRs (ORs) in mammalian olfactory receptor neurons (ORNs) mediate excitation through the Gαs family member Gαolf. Here we tentatively associate a second G protein, Gαo, with inhibitory signaling transduction by first showing that odor evoked phosphoinositide 3-kinase (PI3K)-dependent inhibition of signal is absent native ORNs mice carrying conditional OMP-Cre based knockout Gαo. We then identify an OR from rat are activated octanol cyclic nucleotide and inhibited citral...
Odorants inhibit as well excite primary olfactory receptor neurons (ORNs) in many animal species. Growing evidence suggests that inhibition of mammalian ORNs is mediated by phosphoinositide (PI) signaling through activation 3-kinase (PI3K), and canonical adenylyl cyclase III PI3K interact to provide the basis for ligand-induced selective signaling. As known act concert with phospholipase C (PLC) some cellular systems, question arises whether they work together mediate inhibitory transduction...