A5043915161- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
- Silk-based biomaterials and applications
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
École Polytechnique Fédérale de Lausanne
2016-2023
University of Geneva
2021
Instituto Gulbenkian de Ciência
2011-2014
University of Lisbon
2009
Despite the crucial importance of Hox genes functions during animal development, mechanisms that control their transcription in time and space are not yet fully understood. In this context, it was proposed Hotair, a lncRNA transcribed from within HoxC cluster regulates Hoxd gene expression trans, through targeting Polycomb consecutive transcriptional repression. This activity recently supported by skeletal phenotype mice lacking Hotair function. However, other loss function alleles at locus...
Mammalian Hox gene clusters contain a range of CTCF binding sites. In addition to their importance in organizing TAD border, which isolates the most posterior genes from rest cluster, positions and orientations these sites suggest that may be instrumental selection various subsets contiguous genes, are targets distinct remote enhancers located flanking regulatory landscapes. We examined this possibility by producing an allelic series cumulative cis mutations sites, up abrogation five on one...
Developmental genes are often controlled by large regulatory landscapes matching topologically associating domains (TADs). In various contexts, the associated chromatin backbone is modified specific enhancer–enhancer and enhancer–promoter interactions. We used a TAD flanking mouse HoxD cluster to study how these architectures formed deconstructed once their function achieved. describe this as functional unit, with several sequences acting together elicit transcriptional response. With one...
ABSTRACT Gastruloids have recently emerged as an efficient four-dimensional model for studying some aspects of post-implantation embryonic patterning. They undergo gastrulation-like processes leading to the self-organization into highly reproducible biological objects. Here, we sought uncover molecular and cellular mechanism underlying this remarkable property. We report that competence is associated with a cell-specific coordination Cadherin switch. find N-Cadherin hinders gastruloids...
The actin-Capping Protein heterodimer, composed of the α and β subunits, is a master F-actin regulator. In addition to its role in many cellular processes, Capping acts as main tumor suppressor module Drosophila humans, part, by restricting activity Yorkie/YAP/TAZ oncogenes. We aimed this report understand how both subunits regulate each other vivo. show that levels capping activities must be tightly regulated control consequently growth wing. Overexpressing protein decreases tissue growth,...
ABSTRACT In vertebrates, developmental genes are often controlled by large regulatory landscapes matching the dimensions of topologically associating domains (TADs). various ontogenic contexts, associated constitutive chromatin backbone is modified fine-tuned specific variations in enhancer-enhancer and enhancer-promoter interaction profiles. this work, we take one TADs flanking HoxD gene cluster as a paradigm to address question how these complex architectures formed they de-constructed...
ABSTRACT Mammalian Hox gene clusters contain a range of CTCF binding sites. In addition to their importance in organizing TAD border, which isolates the most posterior genes from rest cluster, positions and orientations these sites suggest that may be instrumental selection various subsets contiguous genes, are targets distinct remote enhancers located flanking regulatory landscapes. We examined this possibility by producing an allelic series cumulative in-cis mutations sites, up abrogation...