A5090034260- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Childhood Cancer Survivors' Quality of Life
- Effects of Radiation Exposure
- Obstructive Sleep Apnea Research
- Nutrition and Health in Aging
- Cardiovascular Disease and Adiposity
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Muscle metabolism and nutrition
- Cancer, Stress, Anesthesia, and Immune Response
- Plant Toxicity and Pharmacological Properties
- Autoimmune and Inflammatory Disorders Research
- Cardiovascular and Diving-Related Complications
- Nematode management and characterization studies
- Congenital Diaphragmatic Hernia Studies
- Sesquiterpenes and Asteraceae Studies
- Natural product bioactivities and synthesis
- Extracellular vesicles in disease
- Urinary Bladder and Prostate Research
- Prostate Cancer Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Thermal Regulation in Medicine
University of Rochester Medical Center
2016-2022
University of Rochester
2015-2022
Neuromuscular junction degeneration is a prominent aspect of sarcopenia, the age-associated loss skeletal muscle integrity. Previously, we showed that stem cells activate and contribute to mouse neuromuscular regeneration in response denervation (Liu et al., 2015). Here, examined gene expression profiles integrity aged muscles, unexpectedly found limited despite high level degenerated junctions. Instead, junctions were associated with reduced contribution from cells. Indeed, cell depletion...
ABSTRACT The functional role of Pax7-expressing satellite cells (SCs) in postnatal skeletal muscle development beyond weaning remains obscure. Therefore, the relevance SCs during prepubertal growth, a period after but prior to onset puberty, has not been examined. Here, we have characterized mouse growth prepuberty and found significant increases myofiber cross-sectional area that correlated with SC-derived myonuclear number. Remarkably, genome-wide RNA-sequencing analysis established...
Abstract Muscle regeneration depends on a robust albeit transient inflammatory response. Persistent inflammation is feature of age-related regenerative deficits, yet the underlying mechanisms are poorly understood. Here, we find inflammatory-related CC-chemokine-receptor 2 (Ccr2) expression in non-hematopoietic myogenic progenitors (MPs) during regeneration. After injury, Ccr2 MPs corresponds to levels its ligands, chemokines Ccl2, 7, and 8. We stimulation Ccr2-activity inhibits MP fusion...
Skeletal muscle regenerative potential declines with age, in part due to deficiencies resident stem cells (satellite cells, SCs) and derived myogenic progenitors (MPs); however, the factors responsible for this decline remain obscure. TGFβ superfamily signaling is an inhibitor of differentiation, elevated activity aged skeletal muscle. Surprisingly, we find reduced expression Smad4, downstream cofactor canonical signaling, target Id1 SCs MPs during regeneration. Specific deletion Smad4 adult...
Objective To examine and quantify the sexual dimorphism in pathologic features manifested musculoskeletal cardiopulmonary systems incidence of mortality tumor necrosis factor–transgenic ( TNF ‐Tg; Tg3647 strain) mouse model inflammatory erosive arthritis. Methods Kaplan‐Meier survival estimates were determined male female mice sex‐matched wild‐type WT ) littermate mice. Longitudinal cross‐sectional outcomes assessed via ultrasound, micro–computed tomography, grip strength measurements,...
During prepubertal development, muscle stem cells (satellite cells, SCs) actively contribute to myofiber growth. Because some SCs are active during this time, they may be particularly susceptible damage. Using a Small Animal Radiation Research Platform (SARRP), we investigated the effects of local fractionated radiation treatment on SCs. Immediately after regimen, there was reduction in SC number. Although surviving had deficiencies function, myogenic potential remained. Indeed, regenerative...
Abstract Background As paediatric cancer survivors are living into adulthood, they suffer from the age‐related, accelerated decline of functional skeletal muscle tissue, termed sarcopenia. With ionizing radiation (radiotherapy) at core therapies, its direct and indirect effects can have lifelong negative impacts on growth maintenance muscle. Utilizing our recently developed preclinical rhabdomyosarcoma mouse model, we investigated late treatment muscles adolescent (8 weeks old) middle‐aged...
Abstract Aims Sarcopenia, the age‐related loss of skeletal muscle, is a side effect androgen deprivation therapy (ADT) for prostate cancer patients. Resident stem cells satellite (SCs), are an essential source progenitors growth and regeneration muscle. Decreased signaling deficits in number function SCs features aging. Although known to regulate cellular basis ADT‐induced exacerbation sarcopenia unknown. Furthermore, consequences on SC fate adult muscle remain largely unexplored. Methods...
Abstract Background Radiotherapy is commonly used to treat childhood cancers and can have adverse effects on muscle function, but the underlying mechanisms yet be fully elucidated. We hypothesized that endurance exercise following radiation treatment would improve skeletal function. Methods utilized Small Animal Radiation Research Platform (SARRP) irradiate juvenile male mice with a clinically relevant fractionated dose of 3× (every other day over 5 days) 8.2 Gy X-ray irradiation locally...
Abstract Pediatric cancer treatment often involves chemotherapy and radiation, where off-target effects can include skeletal muscle decline. The effect of such treatments on juvenile growth has yet to be investigated. We employed a small animal irradiator administer fractionated hindlimb irradiation mice bearing implanted rhabdomyosarcoma (RMS) tumors. Hindlimb-targeted (3 × 8.2 Gy) 4-week-old successfully eliminated RMS tumors one week prior. After establishment this preclinical model,...
Inflammation is a key factor in both influenza and radiation-induced lung pathophysiology. This implies commonality of response to pulmonary damage from these insults suggests exacerbated pathology may occur after combined exposure. We therefore tested the hypothesis that past inflammation viral infection alters microenvironment lowers tolerance for radiation injury. Mice were inoculated with A virus (IAV) three weeks later, clearance, mice received total-body irradiation (TBI). Survival as...