Iron is an essential element for life. However, the iron overload can be toxic. Here, we investigated significant increase of tenellin and iron–tenellin complex production in ferricrocin-deficient mutants Beauveria bassiana. Our chemical analysis indicated that T1, T3 T5 nearly abolished ferricrocin production. In turn, these had accumulation their mycelia at 247–289 mg g−1 cell dry weight under iron-replete condition. Both were not detected wild-type such Mass mutants' crude extracts...

Trichoderma spp. are regularly found as a constituent of the mycoflora many soils and noted for their antagonistic activity against bacteria other fungi. This latter property is basis widespread interest in use biological control soil-borne fungal plant pathogens. antagonism partly based on ability to produce an impressive inventory secondary metabolites. An important group bioactive metabolites produced by non-ribosomal peptides (NRPs), especially peptaibols. A virulent strain, T....

Fungicide resistance now exists in the rice blast fungus, Magnaporthe grisea, necessitating need for new active agents. Fungi isolated from habitats Thailand were screened with reference to this problem.A new, reliable vitro screening system based on a microdilution plate format was set up using virulent strain of M. grisea THL 16. Culture broth extracts approximately 800 fungal strains investigated, one these, Aschersonia luteola BCC 8774, found produce an fungicidal compound,...

The growing demand for natural pigments in the industrial sector is a significant driving force development of production processes. blue pigments, which have wide applications, using microbial systems has been gaining attention. In this study, we used Aspergillus oryzae as platform cell factory to produce pigment indigoidine (InK), by genetic manipulation its non-ribosomal peptide synthetase system overexpress gene (AoinK). Phenotypic analysis showed that InK from engineered strain was...

A novel endophytic actinomycete, designated strain SC1-1T, was isolated from sterilized stem tissue Stahlianthus campanulatus collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented meso-diaminopimelic acid the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose ribose were observed as sugars cells. membrane phospholipids phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine,...

A novel Gram-stain-positive, non-motile, endophytic actinomycete, designated strain BR3-1T, which produced spore chains borne on the tips of short sporophores, was isolated from rhizome Boesenbergia rotunda collected Udon Thani province, Thailand. This investigated for its taxonomic position using a polyphasic approach. The contained 3-hydroxydiaminopimelic acid and meso-diaminopimelic in cell-wall peptidoglycan. whole-cell sugars comprised glucose, mannose, rhamnose, ribose xylose....

Cordycepin (3'-deoxyadenosine) is an important bioactive compound in medical and healthcare markets. The drawbacks of commercial cordycepin production using Cordyceps spp. include long cultivation periods low yields. To overcome these limitations meet the increasing market demand, efficient by GRAS-status Aspergillus oryzae strain a synthetic biology approach was developed this study.An engineered A. capable successfully constructed overexpressing two metabolic genes (cns1 cns2) involved...

Severe chili anthracnose disease in Thailand is caused by Colletotrichum gloeosporioides and C. capsici. To discover anti-anthracnose substances we developed an efficient dual-fluorescent labeling bioassay based on a microdilution approach. Indicator strains used the assay were constructed integrating synthetic green fluorescent protein (sGFP) Discosoma sp. red (DsRedExp) genes into genomes of or capsici respectively. Survival co-spore cultures presence inhibitors was determined expression...

A novel endophytic actinomycete, designated strain KK1-3T, which formed single spores and long chains of (more than 10 spores) was isolated from surface-sterilized Kaempferia larsenii leaf collected Ubon Ratchathani province, Thailand. The isolate contained l-lysine, meso-diaminopimelic acid hydroxyl diaminopimelic in the cell-wall peptidoglycan. whole-cell sugars included glucose, mannose, rhamnose, ribose, galactose xylose. characteristic phospholipids were phosphatidylethanolamine,...

The potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptome-based approach, novel pentose-regulated of the xylose reductase gene (PxyrA) Aspergillus oryzae was identified. analysis showed that PxyrA tightly regulated by pentose sugars, which xylan were favorable inducers. function highly efficient as compared with maltose-inducible promoters A. oryzae. It also exhibited transcription induction even though certain amounts...

Ammonium is a source of fermentable inorganic nitrogen essential for the growth and development filamentous fungi. It involved in several cellular metabolic pathways underlying transport assimilation. can be transferred into cell by an ammonium transporter. This study explored role transporters metabolism biomass production

Many species of Trichoderma have attracted interest as agents for the biological control soil borne fungal pathogens a range crop plants. Research on biochemical mechanisms associated with this application has focused ability these fungi to produce enzymes which lyse cell walls, and antifungal antibiotics. An important group latter are non-ribosomal peptides called peptaibols. In study asperellum, strain used in Malaysia, was found peptaibol, trichotoxin. This type peptide molecule is...

An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach used for evaluating the taxonomic position this strain. affiliation at genus level could be confirmed by its chemotaxonomic characteristic, i.e. presence ll-diaminopimelic acid in cell peptidoglycan, MK-9(H4) as major menaquinone, iso-C16 : 0, anteiso-C15 iso-C14 0 and iso-C15 predominant fatty acids cells, diphosphatidylglycerol,...

The ∆6-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential pharmaceutical and nutraceutical applications. gene been isolated from selected strain Oomycetes, Pythium sp. BCC53698. cloned (PyDes6) contained an open reading frame (ORF) 1401 bp 466 amino acid residues. deduced sequence shared high similarity to those other ∆6-desaturases that signature features membrane-bound ∆6-desaturase, including cytochrome b 5 three histidine-rich motifs...