Abstract Background Up to now, there have been three published versions of a yeast genome-scale metabolic model: iFF708 , iND750 and iLL672 . All models, however, lack detailed description lipid metabolism thus are unable be used as integrated scaffolds for gaining insights into from multilevel omic measurement technologies (e.g. genome-wide mRNA levels). To overcome this limitation, we reconstructed new version the Saccharomyces cerevisiae model, iIN800 that includes more rigorous...

For a bio-based economy, microbial lipids offer potential solution as alternative feedstocks in the oleochemical industry. The existing genome data for promising strains, oleaginous yeasts and fungi, allowed us to investigate candidate orthologous sequences that participate their oleaginicity. Comparative analysis of non-oleaginous (Saccharomyces cerevisiae, Candida albicans Ashbya gossypii) strains (Yarrowia lipolytica, Rhizopus oryzae, Aspergillus oryzae Mucor circinelloides) showed 209...

An optimal culture medium and highly stable biometabolites are important in industrial production processes. The response surface methodology with a Box–Behnken design was performed to determine the of an engineered Aspergillus oryzae strain for cordycepin by submerged fermentation. influences glucose, yeast extract, adenine concentrations on were explored, their used experimental design. results reveal that components involved 30.0 g/L 9.8 1.5 adenine. As predicted, maximum concentration...

Research into the cellular metabolic adaptations of Mucor circinelloides has gained significant interest due to its capability for lipid production, which critical industrial applications. To address regulatory mechanisms at systems level, this study aimed explore global responses associated with production in high and low lipid-producing strains M. circinelloides, WJ11 CBS277.49, respectively, through comparative transcriptome analysis genome-scale model-driven analysis. The expressed genes...

Abstract Due to their health benefits there is much interest in developing microbial processes for efficient production of polyunsaturated fatty acids (PUFAs). In this study we co‐expressed Mucor rouxii Δ 12 ‐ and 6 ‐desaturase genes Saccharomyces cerevisiae , which resulted a yeast strain that accumulated linoleic acid γ‐linolenic the different lipid species. Additionally, contained higher levels phospholipids lower ergosterol than reference strain. Integrated analysis transcriptome...

Abstract The cellular metabolic adaptations of Cordyceps militaris have been progressively studied. In particular, the cordycepin pathway is interest in medicinal applications. Even though pathways for production are known to be related different carbon sources, regulatory mechanisms at a systems level poorly characterized. To explore mechanisms, this study therefore aimed investigate global response C. through transcriptome analysis and genome-scale network-driven analysis. Here, 16,805...

Aims: This study aims to maximize the yield of gamma-linolenic acid by a filamentous fungus, Mucor rouxii, using low cost production solid-state fermentation. Methods and Results: We optimized substrate types culture conditions including inoculum size temperature. The optimal growth M. rouxii was found in cultures inoculated with 5 × 105 spores g−1 substrate. fungal cells grew well on rice bran soy bean meal, whereas lower biomass grown polished rice, broken spent malt grain. GLA content...

The genome-scale metabolic model (GSMM) of Cordyceps militaris provides a comprehensive basis carbon assimilation for cell growth and metabolite production. However, the with simple mass balance concept shows limited capability to probe responses C. under light exposure. This study, therefore, employed transcriptome-integrated GSMM approach extend investigation militaris’s metabolism conditions. Through gene inactivity moderated by expression (GIMME) framework, iPS1474-tiGSMM was furnished...

To identify the promising oleaginous Aspergillus oryzae strain and leverage its lipid biomass production through a mathematical model.

To understand the relationship between fatty acid metabolism and growth morphology of Mucor rouxii, profiling was studied comparatively in cells grown under conditions which included different atmospheric or addition phenethyl alcohol (PEA). The significant difference profiles from M. rouxii aerobic anaerobic not found to be directly related morphological growth. Oxygen limitation, induced formation pure multipolar budding yeasts, led a decrease long-chain acids-- particularly unsaturated an...

Abstract Within a metabolic network, the elementary flux modes enables unique description of different operations network. Thus, fluxes can be specified as convex combinations modes. Here, we describe an approach to identify set that operates in given network through use measurements macroscopic fluxes, is, and out cell. Besides enabling estimation parameters linear provide valuable physiological information; call these regulation coefficients (FRCs). These indicate which enzyme subsets are...

The growing demand for natural pigments in the industrial sector is a significant driving force development of production processes. blue pigments, which have wide applications, using microbial systems has been gaining attention. In this study, we used Aspergillus oryzae as platform cell factory to produce pigment indigoidine (InK), by genetic manipulation its non-ribosomal peptide synthetase system overexpress gene (AoinK). Phenotypic analysis showed that InK from engineered strain was...