A5057273462- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Animal Genetics and Reproduction
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Vector-borne infectious diseases
- Vaccine Coverage and Hesitancy
- Endoplasmic Reticulum Stress and Disease
- Trypanosoma species research and implications
- Autophagy in Disease and Therapy
- Science and Education Research
- Oral Health Pathology and Treatment
- Pancreatic function and diabetes
- Social and Political Issues
- Signaling Pathways in Disease
- Vector-Borne Animal Diseases
- Biological Research and Disease Studies
- Polyamine Metabolism and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Insect-Plant Interactions and Control
Universidade Federal Fluminense
2001-2023
Instituto Nacional do Câncer
2023
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2015-2022
Universidade Federal do Rio de Janeiro
1998-2021
Universidade Federal de Minas Gerais
2020-2021
Escola Superior de Ciências da Saúde
2020
Prefeitura Municipal de Campos dos Goytacazes
2009-2015
State University of Norte Fluminense
2009-2014
Universidade Federal do Acre
2012
Fundação Oswaldo Cruz
2012
An aspartic proteinase precursor, herein named BYC ( Boophilus Yolk pro-Cathepsin) was isolated from eggs of the hard tick, microplus . As judged by electrophoresis on sodium dodecyl sulfate polyacrylamide slab gel (SDS–PAGE), purified presented 2 bands 54 and 49 kDa, bearing same NH -terminal amino acid sequence. By Western blot analysis, also found in haemolymph, indicating an extraovarian site synthesis. Several organs were incubated culture medium with [ 35 S] methionine, only gut fat...
Abstract Background The mosquito A. aegypti is vector of dengue and other viruses. New methods control are needed can be achieved by a better understanding the life cycle this insect. Embryogenesis part aegypty that poorly understood. In insects in general mosquitoes particular energetic metabolism well studied during oogenesis, when oocyte exhibits fast growth, accumulating carbohydrates, lipids proteins will meet regulatory metabolic needs developing embryo. On hand, events related with...
Periodontal disease may be associated with more bacteria and consequent induction of a systemic inflammatory process, changes in the levels C-reactive protein (CRP).The purpose this cross-sectional study was to evaluate association between periodontitis serum protein.Material Methods: The sample comprised 100 individuals distributed into two groups according protein: normal or altered.Social, biological behavioral data were collected by means structured questionnaire.Additionally, blood test...
Wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. is natural endosymbiont Aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. In this context, we investigated correlation between glucose metabolism morphological alterations during A. fluviatilis embryo development Wolbachia-positive (W+) Wolbachia-negative (W−) mosquito strains. While...
Tick embryogenesis is a metabolically intensive process developed under tightly controlled conditions and whose components are poorly understood.In order to characterize the role of AKT (protein kinase B) in glycogen metabolism cell viability, determination, identification cloning an from Rhipicephalus microplus were carried out, parallel with experiments using RNA interference (RNAi) chemical inhibition.A decrease content was observed when chemically inhibited by 10-DEBC treatment, while...
In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism anabolism, BME26 Rhipicephalus microplus embryonic cell line. Genetic catalytic control of enzymes associated with these are modulated by alterations energy resource availability (primarily glucose). cells media were investigated using three different glucose concentrations, changes transcription levels target response to utilization...
Reactive oxygen species (ROS) are natural byproducts of metabolism that have toxic effects well documented in mammals. In hematophagous arthropods, however, these processes not largely understood. Here, we describe Rhipicephalus microplus ticks and embryonic cell line (BME26) employ an adaptive metabolic compensation mechanism confers tolerance to hydrogen peroxide (H2O2) at concentrations too high for others organisms. Tick survival reproduction affected by H2O2 exposure, while BME26 cells...
SUMMARY Rhodnius prolixus is a blood-feeding insect that transmits Trypanosoma cruzi and rangeli to vertebrate hosts. also classical model in physiology, the recent availability of R. genome has opened new avenues on triatomine research. Glycogen synthase kinase 3 (GSK-3) classically described as key enzyme involved glycogen metabolism, acting downstream component Wnt pathway during embryogenesis. GSK-3 been shown be highly conserved among several organisms, mainly catalytic domain region....
Abstract In the present work, we established two novel embryonic cell lines from mosquito Aedes fluviatilis containing or not naturally occurring symbiont bacteria Wolbachia, which were called w Aflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate symbiont-host interaction in context of energy metabolism regulation molecular mechanisms immune responses...
RNA interference-mediated gene silencing was shown to be an efficient tool for validation of targets that may become anti-tick vaccine components. Here, we demonstrate the application this approach in components molecular signaling cascades, such as Protein Kinase B (AKT) / Glycogen Synthase (GSK) axis during tick embryogenesis. It heptane and hypochlorite treatment eggs can remove wax, affecting corium integrity but not embryo development. Evidence AKT GSK dsRNA delivery into de-waxed via...
Embryogenesis is a metabolically intensive process carried out under tightly controlled conditions. The insulin signaling pathway regulates glucose homeostasis and essential for reproduction in metazoan model species. Three key targets are part of this pathway: protein kinase B (PKB, or AKT), glycogen synthase 3 (GSK-3), target rapamycin (TOR). While the role AKT GSK-3 has been investigated during tick embryonic development, TOR remains unknown. In study, two other downstream effectors,...
Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role maintaining physiological homeostasis and preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance to H2O2 exposure. Here, describe Rhipicephalus microplus tick line (BME26) employs adaptive glucose mechanism confers hydrogen peroxide at concentrations too for other organisms. This sustained remodeling...
Most epidemiological studies involving oral and maxillofacial lesions assess only data from histopathological analysis. This may lead to a poor notification of diseases whose diagnosis is predominantly clinical. Aim: To evaluate compare the frequency in children adolescents two different types services: Oral Medicine clinic service laboratory service.The records patients ≤ 19 years attending Maxillofacial Pathology Dental School university were analyzed.828 2,409 analyzed. The most common...
Mobile phones obtained large popularization in Brazil thanks to pre-paid (pay as you go) contracts. This kind of contract represents 82% mobile phone connections Brazil, corresponding 143 million a total 174 accesses the country. In this scenario, research about usability with 17 24 year old youngsters, living poor communities, enrolled NGO Spectaculu. Usability tests were carried through 30 youngsters from Rio de Janeiro. Was made extraction navigation sequence data information input and...