A5068855855- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Epilepsy research and treatment
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Neurological disorders and treatments
- Parkinson's Disease Mechanisms and Treatments
- Sulfur Compounds in Biology
- Biochemical effects in animals
- Signaling Pathways in Disease
- Nuclear Receptors and Signaling
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
- Lipid Membrane Structure and Behavior
- Neuroendocrine regulation and behavior
- Genomics and Phylogenetic Studies
- Biopolymer Synthesis and Applications
- Vitamin C and Antioxidants Research
- Advanced Memory and Neural Computing
- Biochemical Analysis and Sensing Techniques
- Enterobacteriaceae and Cronobacter Research
University of Patras
2011-2024
Brain Physiology Lab
2005
Alexandra Hospital
1976-1982
National and Kapodistrian University of Athens
1976
To elucidate the consequences of convulsions, we examined biochemically and electrophysiologically brains mice that had sustained two complete tonic-clonic convulsions after administration pentylenetetrazol (PTZ 50 mg/kg intraperitoneally, i.p.), 48 24 h before decapitation. Control were injected with saline. Input/output curves extracellular synaptic responses in CA1 area hippocampal slices showed PTZ-induced seizures do not establish persistent change excitability itself can be detected...
Abstract A great body of evidence points toward a functional interaction between metabotropic glutamate 5 receptors ( mG luR5) and NMDA NMDAR ) that enhances synaptic plasticity cognition. However, the molecular mechanism underlying this remains unclear. Here, we show co‐activation luR5 in hippocampal slices synergistically leads to robust phosphorylation NR 2B (Tyr1472), which is Src kinase dependent enabled by endogenous adenosine acting on 2A receptors. As it well known, (Tyr1472) anchors...
Abstract Recent data demonstrate weaker γ‐aminobutyric acid (GABA)‐ergic inhibition in ventral (VH) compared with dorsal (DH) hippocampus. Therefore, we examined possible differences regarding the GABA A receptors between VH and DH as follows: 1) expression of receptor subunits (α1/2/4/5, β1/2/3, γ2, δ) mRNA protein 2) quantitative distribution kinetic parameters [ 3 H]muscimol (GABA agonist) binding. showed: lower levels for α1, β2, γ2 but higher α2 β1 CA1, CA2, CA3, being more pronounced...
Age related changes in A1 adenosine receptor binding were investigated mouse brain using the selective agonist, [3H]-cyclohexyladenosine ([3H]CHA). In cortex, hippocampus and cerebellum of aged mice (28 months old), a significant decrease about 44%, 50% 12%, respectively, [3H]CHA compared to young animals (3 old) was observed. According Scatchard analysis data this due density reduction not Kd change. Since weight protein content each tissue tested did differ significantly between old...
Summary: Purpose: In the present study, we examined effects of pentylenetetrazol (PTZ) administration on thiol redox state (TRS), lipid peroxidation, and protein oxidation in mouse striatum to (a) quantitate major components TRS relate them oxidative stress, (b) investigate whether neuronal activation without synchronization, induced by subconvulsive doses PTZ, can cause similar qualitative this brain area. Specifically, glutathione (GSH), disulfide (GSSG), cysteine (CSH), thiols (PSH), (P)...
Abstract Background Loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) underlines much pathology Parkinson’s disease (PD), but existence an endogenous neurogenic system that could be targeted as a therapeutic strategy has been controversial. BNN-20 is synthetic, BDNF-mimicking, microneurotrophin we previously showed to exhibit pleiotropic neuroprotective effect on SNpc “weaver” mouse model PD. Here, assessed its potential effects neurogenesis. Methods We quantified...
Ageing is a multifactorial, inevitable event of life span, which affects neurotransmission in the CNS. Since adenosine major neuromodulator synaptic activity, it was interest to investigate possible modification adenosinergic system brain during ageing. Using “in vitro” quantitative autoradiography and radioactive ligands [3H]Cyclohexyladenosine [3H]Nitrobenzylthioinosine, we have studied distribution A1 receptors uptake sites aged mice (26 months) compared young ones (3 months). Our results...
The possible involvement of the adenosinergic modulatory system in pathogenesis absence seizures was investigated genetic epilepsy rats from Strasbourg (GAERS). Using vitro quantitative autoradiography, distribution A1 adenosine receptors and uptake sites brain GAERS studied compared to that control animals. An area-specific lower density (15% decrease) detected reticular (nRT) anterior ventral (AV) thalamic nuclei as well basal ganglia brains animals with Since exerts an anti-oscillatory...
Abstract Interaction between mGluR5 and NMDA receptors ( NMDAR ) is vital for synaptic plasticity cognition. We recently demonstrated that stimulation of enhances responses in hippocampus by phosphorylating NR2B(Tyr1472) subunit, this reaction was enabled adenosine A 2A (A R) (J Neurochem, 135, 2015, 714). In study, using vitro phosphorylation western blot analysis hippocampal slices male Wistar rats, we show or mGluR5/ s co‐stimulation synergistically activate ERK 1/2 signaling leading to...
Adenosine has been shown to play a significant role as modulator of neuronal activity in convulsion disorders, acting an endogenous anticonvulsant agent. In the present study, we have investigated mice effect acute tonic-clonic seizures induced by single Pentylenetetrazol (PTZ)-injection (a) on time development adenosine uptake site binding after membranes hippocampus, cortex, cerebellum, and striatum, (b) regional distribution sites mouse brain using "in vitro" quantitative autoradiography....