A5042605431- Fibroblast Growth Factor Research
- Alzheimer's disease research and treatments
- Advanced Fluorescence Microscopy Techniques
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Proteoglycans and glycosaminoglycans research
- Metastasis and carcinoma case studies
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Gastric Cancer Management and Outcomes
- Advanced Electron Microscopy Techniques and Applications
- Skin and Cellular Biology Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Diverse Aspects of Tourism Research
- Photoacoustic and Ultrasonic Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Dementia and Cognitive Impairment Research
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Optical Coherence Tomography Applications
- Endoplasmic Reticulum Stress and Disease
- Brain Tumor Detection and Classification
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Gallbladder and Bile Duct Disorders
- Cholinesterase and Neurodegenerative Diseases
UK Dementia Research Institute
2021-2024
University of Cambridge
2021-2024
Heidelberg University
2014-2022
Heidelberg University
2019-2022
University of Patras
2017-2021
European Molecular Biology Laboratory
2015
Aristotle University of Thessaloniki
2011-2012
Vienna General Hospital
2009
Tzaneion General Hospital
2006
Federation of Greek Mariculture
1981
The progressive deposition of misfolded hyperphosphorylated tau is a pathological hallmark tauopathies, including Alzheimer's disease. However, the underlying molecular mechanisms governing intercellular spreading species remain elusive. Here, we show that full-length soluble unconventionally secreted by direct translocation across plasma membrane. Increased secretion favored hyperphosphorylation, which provokes microtubule detachment and increases availability free protein inside cells....
Which isoforms of apolipoprotein E (apoE) we inherit determine our risk developing late-onset Alzheimer's Disease (AD), but the mechanism underlying this link is poorly understood. In particular, relevance direct interactions between apoE and amyloid-β (Aβ) remains controversial. Here, single-molecule imaging shows that all associate with Aβ in early stages aggregation then fall away as fibrillation happens. ApoE-Aβ co-aggregates account for ~50% mass diffusible aggregates detected frontal...
Transcription factors of the far‐upstream element‐binding protein (FBP) family represent cellular pathway hubs, and their overexpression in liver cancer (hepatocellular carcinoma [HCC]) stimulates tumor cell proliferation correlates with poor prognosis. Here we determine mode oncogenic FBP HCC cells. Using perturbation approaches (kinase inhibitors, small interfering RNAs) a novel system for rapalog‐dependent activation AKT isoforms, demonstrate that activity...
Tau is a soluble protein interacting with tubulin to stabilize microtubules. However, under pathological conditions, it becomes hyperphosphorylated and aggregates, process that can be induced by treating cells exogenously added tau fibrils. Here, we employ single-molecule localization microscopy resolve the aggregate species formed in early stages of seeded aggregation. We report entry sufficient assemblies into cytosol induces self-replication small doubling time 5 h inside HEK 1 day murine...
<b>Background and study aims:</b> Pancreatitis is the most common complication of therapeutic endoscopic retrograde cholangiopancreatography (ERCP), many pharmacoprophylactic approaches have been suggested, though not without controversy. The aim was to investigate impact combined therapy with diclofenac plus somatostatin on reducing frequency severity post-ERCP pancreatitis (PEP). <b>Patients methods:</b> A prospective, double-blind, placebo-controlled trial conducted in two tertiary...
Fibroblast growth factor 2 (FGF2) is a key signaling molecule in tumor-induced angiogenesis. FGF2 secreted by an unconventional secretory mechanism that involves phosphatidylinositol 4,5-bisphosphate-dependent insertion of oligomers into the plasma membrane. This process regulated Tec kinase-mediated tyrosine phosphorylation FGF2. Molecular interactions driving monomers membrane-inserted are unknown. Here we identify two surface cysteines critical for efficient secretion They represent...
Background: Normal saline (NS) plus epinephrine (E) is the traditionally used solution as submucosal fluid cushion for a safe and effective endoscopic mucosal resection (EMR) of sessile colorectal polyps. It was hypothesized that hydroxyethyl starch (HES), an inexpensive easily available might be ideal prolonged elevation easy EMR giant lateral spreading tumors (LSTs). Patients Methods: During 6-year period, patients suffering from LSTs with diameter ≥30 mm were randomized to undergo by...
FGF2 is exported from cells by an unconventional secretory mechanism. Here, we directly visualized individual membrane translocation events at the plasma using live cell TIRF microscopy. This process was dependent on both PI(4,5)P2–mediated recruitment of inner leaflet and heparan sulfates capturing outer leaflet. By simultaneous imaging appearance surface, revealed kinetics in living with average duration ∼200 ms. Furthermore, demonstrated oligomers a dimer being most prominent species. We...
Fibroblast growth factor 2 (FGF2) is a tumor cell survival that transported into the extracellular space by an unconventional secretory mechanism. Cell surface heparan sulfate proteoglycans are known to play essential role in this process. Unexpectedly, we found among diverse subclasses consisting of syndecans, perlecans, glypicans, and others, Glypican-1 (GPC1) principle rate-limiting drives secretion FGF2. By contrast, demonstrate GPC1 be dispensable for FGF2 signaling cells. We provide...
Abstract FGF2 is a tumor cell survival factor that exported from cells by an ER/Golgi-independent secretory pathway. This unconventional mechanism of protein secretion based on direct translocation across the plasma membrane. The Na,K-ATPase has previously been shown to play role in this process, however, underlying remained elusive. Here, we define structural elements are critical for physical interaction between and α1 subunit Na,K-ATPase. In intact cells, corresponding mutant forms were...
Super-resolution microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays. Methods developed circumvent this problem are often expensive, hard implement, not applicable total internal reflection fluorescence imaging. We herein demonstrate a cost-effective method overcome these challenges using small square-core multimodal optical...
Abstract Background Patients with diabetes already fulfill one diagnostic criterion for MS according to the existing classifications. Our aim was identify single clinical parameter, which could effectively predict presence of in patients type 2 diabetes. Methods We studied all who attended our Diabetes Outpatient Clinic during a three-month period. Waist circumference, blood pressure and serum lipids were measured. Establishment diagnosis based a) on National Cholesterol Education Program...
Mesenteric panniculitis (MP) is a rare inflammatory and fibrotic disease of the mesentery unknown etiology. It has various clinical radiological manifestations, posing diagnostic challenge for clinicians. Its diagnosis indicated via radiologic imaging usually confirmed peritoneal biopsies. We describe case patient with histopathologically proven MP, in which steroid dependence was successfully managed colchicine.
Abstract Interaction between mGluR5 and NMDA receptors ( NMDAR ) is vital for synaptic plasticity cognition. We recently demonstrated that stimulation of enhances responses in hippocampus by phosphorylating NR2B(Tyr1472) subunit, this reaction was enabled adenosine A 2A (A R) (J Neurochem, 135, 2015, 714). In study, using vitro phosphorylation western blot analysis hippocampal slices male Wistar rats, we show or mGluR5/ s co‐stimulation synergistically activate ERK 1/2 signaling leading to...
Reprogramming somatic cells into induced-pluripotent (iPSCs) provides new access to all cell types for clinical application without any ethical controversy arising from the use of embryonic stem (ESCs). Established protocols iPSCs generation based on viral transduction with defined factors are limited by low efficiency and risk genetic abnormality. Several small molecules have been reported as replacements transcriptional factors, but a chemical able replace Oct3/4 allowing human is still...
Abstract Fibroblast Growth Factor 2 (FGF2) is a tumor cell survival factor that transported into the extracellular space by an unconventional secretory mechanism. Cell surface heparan sulfate proteoglycans are known to play essential role in this process. Unexpectedly, we found among diverse sub-classes consisting of syndecans, perlecans, glypicans and others, Glypican-1 (GPC1) both principle rate-limiting drives secretion FGF2. By contrast, demonstrate GPC1 be dispensable for FGF2 signaling...