A5062029313- Malaria Research and Control
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Fibroblast Growth Factor Research
- Proteoglycans and glycosaminoglycans research
- Aquaculture disease management and microbiota
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Prion Diseases and Protein Misfolding
- Drug Transport and Resistance Mechanisms
- Evolution and Genetic Dynamics
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Computational Drug Discovery Methods
- RNA Research and Splicing
- Skin and Cellular Biology Research
- Cancer, Hypoxia, and Metabolism
- Viral-associated cancers and disorders
- Advanced Fluorescence Microscopy Techniques
- Systemic Lupus Erythematosus Research
- Genetic diversity and population structure
- Lymphoma Diagnosis and Treatment
- Viral Infections and Immunology Research
- Photosynthetic Processes and Mechanisms
- Research on Leishmaniasis Studies
University Hospital Heidelberg
2020-2024
Heidelberg University
2018-2024
Heidelberg University
2021-2022
Nagasaki University
2020-2021
German Cancer Research Center
2018
DKFZ-ZMBH Alliance
2018
Malaria-causing parasites proliferate within erythrocytes through schizogony, forming multinucleated stages before cellularization. Nuclear multiplication does not follow a strict geometric 2
Fibroblast growth factor 2 (FGF2) is a tumor cell survival that transported into the extracellular space by an unconventional secretory mechanism. Cell surface heparan sulfate proteoglycans are known to play essential role in this process. Unexpectedly, we found among diverse subclasses consisting of syndecans, perlecans, glypicans, and others, Glypican-1 (GPC1) principle rate-limiting drives secretion FGF2. By contrast, demonstrate GPC1 be dispensable for FGF2 signaling cells. We provide...
The Epstein-Barr virus (EBV) genome encodes several hundred transcripts. We have used ribosome profiling to characterize viral translation in infected cells and map new initiation sites. show here that EBV transcripts are translated with highly variable efficiency, owing transcription rates, recruitment the leader region coverage by monosomes versus polysomes. Some were hardly translated, others mainly carried monosomes, showed accumulation regions most likely represent non-coding RNAs. A...
Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe development a semi-quantitative high-content microscopy-based assay for detection three major classes (IgG, IgA, IgM) specific antibodies in human samples. The possibility to detect against entire viral proteome together with robust semi-automated image analysis workflow resulted specific, sensitive unbiased complements portfolio...
ABSTRACT Plasmodium falciparum proliferates through schizogony in the clinically relevant blood stage of infection. During schizogony, consecutive rounds DNA replication and nuclear division give rise to multinucleated stages before cellularization occurs. Although these nuclei reside a shared cytoplasm, occur asynchronously. Here, by mapping proteomic context S-phase-promoting kinase Pf CRK4, we show that it has dual role for nuclear-cycle progression: CRK4 orchestrates not only...
Rodent malaria parasites (RMPs) serve as tractable tools to study parasite biology and host-parasite-vector interactions. Among the four RMPs originally collected from wild thicket rats in sub-Saharan Central Africa adapted laboratory mice, Plasmodium vinckei is most geographically widespread with isolates five separate locations. However, there a lack of extensive phenotype genotype data associated this species, thus hindering its use experimental studies.We have generated comprehensive...
Centrins are small calcium-binding proteins that have a variety of roles and universally associated with eukaryotic centrosomes. Rapid proliferation the malaria-causing parasite Plasmodium falciparum in human blood depends on particularly divergent acentriolar centrosome, which incorporates several essential centrins. Their precise mode action, however, remains unclear. In this study calcium-inducible liquid-liquid phase separation is revealed as an evolutionarily conserved principle...
Decreased susceptibilities of the human malaria parasite Plasmodium falciparum towards endoperoxide antimalarial artemisinin are linked to mutations residue C580 PfKelch13, a homologue redox sensor Keap1 and other vertebrate BTB-Kelch proteins. Here, we addressed whether alter susceptibility by modifying properties PfKelch13 or compromising its native fold abundance. Using selection-linked integration glmS ribozyme, efficient down-regulation resulted in ring-stage survival rates around 40%....
Human immunodeficiency virus (HIV)-1 assembly is initiated by Gag binding to the inner leaflet of plasma membrane (PM). targeting mediated its N-terminally myristoylated matrix (MA) domain and PM phosphatidylinositol 4,5-bisphosphate [PI(4,5)P
Abstract Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents numerous questions challenges that demand immediate attention. Among these is urgent need for a better understanding humoral immune response against virus as basis developing public health strategies to control viral spread. For this, sensitive, specific quantitative serological assays are required. Here we describe development semi-quantitative high-content microscopy-based assay...
Abstract Rapid proliferation of the malaria-causing parasite Plasmodium falciparum in human blood depends on a particularly divergent and acentriolar centrosome, which incorporates several essential centrins. Centrins are small calcium-binding proteins that have variety roles universally associated with eukaryotic centrosomes. Their precise mode action, however, remains unclear. In this study calcium-inducible liquid-liquid phase separation is revealed as an evolutionary conserved principle...
Abstract Plasmodium falciparum proliferates through schizogony in the clinically relevant blood stage of infection. During schizogony, consecutive rounds DNA replication and nuclear division give rise to multinucleated stages before cellularization occurs. Although these nuclei reside a shared cytoplasm, occur asynchronously. Here, by mapping proteomic context S-phase-promoting kinase Pf CRK4, we show that it has dual role for nuclear-cycle progression: CRK4 orchestrates not only...
Malaria-causing parasites proliferate within erythrocytes through schizogony, forming multinucleated stages before cellularization. Nuclear multiplication does not follow a strict geometric 2 n progression and each proliferative cycle produces heterogeneous number of progeny. Here, by tracking nuclei DNA replication, we show that individual replicate their at different times, despite residing in shared cytoplasm. Extrapolating from experimental data using mathematical modeling, demonstrate...
Expansion microscopy has recently emerged as an alternative technique for achieving high-resolution imaging of biological structures. Improvements in resolution are achieved by physically expanding samples through embedding a swellable hydrogel before microscopy. However, expansion been rarely used the field virology. Here, we evaluate and characterize ultrastructure (U-ExM) protocol, which facilitates approximately four-fold sample expansion, enabling visualization different post-entry...
Abstract Fibroblast Growth Factor 2 (FGF2) is a tumor cell survival factor that transported into the extracellular space by an unconventional secretory mechanism. Cell surface heparan sulfate proteoglycans are known to play essential role in this process. Unexpectedly, we found among diverse sub-classes consisting of syndecans, perlecans, glypicans and others, Glypican-1 (GPC1) both principle rate-limiting drives secretion FGF2. By contrast, demonstrate GPC1 be dispensable for FGF2 signaling...
Abstract Background Rodent malaria parasites (RMPs) serve as tractable tools to study parasite biology and host-parasite-vector interactions. Plasmodium vinckei is the most geographically widespread of four RMP species collected in sub-Saharan Central Africa. Several P. isolates are available but relatively less characterized than other RMPs, thus hindering their use experimental studies. We have generated a comprehensive resource for comprising high-quality reference genomes, genotypes,...
Abstract Decreased susceptibilities of Plasmodium falciparum towards the endoperoxide antimalarial artemisinin are linked to mutations residue C580 Kelch13, which is homologue redox sensor Keap1 in vertebrates. Here, we addressed whether alter susceptibility by modifying properties Kelch13 or compromising its native fold abundance. Using selection-linked integration and glmS ribozyme, efficient down-regulation resulted ring-stage survival rates around 40%. While loss a potential disulfide...