A5025930111- Marine Invertebrate Physiology and Ecology
- Lipid Membrane Structure and Behavior
- Marine Toxins and Detection Methods
- Erythrocyte Function and Pathophysiology
- Marine Sponges and Natural Products
- interferon and immune responses
- Nanopore and Nanochannel Transport Studies
- Cell death mechanisms and regulation
- Antimicrobial Peptides and Activities
- Ferroptosis and cancer prognosis
- Venomous Animal Envenomation and Studies
- Bacteriophages and microbial interactions
- Marine and environmental studies
- Trace Elements in Health
- Inflammasome and immune disorders
- Protist diversity and phylogeny
- Heme Oxygenase-1 and Carbon Monoxide
- Cell Image Analysis Techniques
- Hemoglobin structure and function
- Acute Myeloid Leukemia Research
- Advanced Fluorescence Microscopy Techniques
- Marine Ecology and Invasive Species
- Coral and Marine Ecosystems Studies
- Cancer, Lipids, and Metabolism
- Caveolin-1 and cellular processes
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2025
University of Cologne
2020-2025
Max Planck Institute of Biophysics
2025
University of Havana
2013-2024
University of Tübingen
2015-2021
Institute for Information Management Bremen
2019
Max Planck Society
2015
Ferroptosis is an iron-dependent form of regulated necrosis associated with lipid peroxidation. Despite its key role in the inflammatory outcome ferroptosis, little known about molecular events leading to disruption plasma membrane during this type cell death. Here we show that a sustained increase cytosolic Ca2+ hallmark ferroptosis precedes complete bursting cell. We report damage nanopores few nanometers radius and but not peroxidation, can be delayed by osmoprotectants. Importantly,...
Necroptosis is a form of regulated necrosis that results in cell death and content release after plasma membrane permeabilization. However, little known about the molecular events responsible for disruption membrane. Here, we find early increase cytosolic calcium TNF-induced necroptosis mediated by treatment with Smac mimetic via TNF/RIP1/TAK1 survival pathway. This does not require activation necrosome dispensable necroptosis. induced TLR3/4 pathways trigger flux. We also demonstrate...
α-Pore-forming toxins (α-PFTs) are ubiquitous defense tools that kill cells by opening pores in the target cell membrane. Despite their relevance host/pathogen interactions, very little is known about pore stoichiometry and assembly pathway leading to membrane permeabilization. Equinatoxin II (EqtII) a model α-PFT from sea anemone oligomerizes forms sphingomyelin-containing membranes. Here, we determined spatiotemporal organization of EqtII living single molecule imaging. Surprisingly, found...
FGF2 is exported from cells by an unconventional secretory mechanism. Here, we directly visualized individual membrane translocation events at the plasma using live cell TIRF microscopy. This process was dependent on both PI(4,5)P2–mediated recruitment of inner leaflet and heparan sulfates capturing outer leaflet. By simultaneous imaging appearance surface, revealed kinetics in living with average duration ∼200 ms. Furthermore, demonstrated oligomers a dimer being most prominent species. We...
Sticholysin I (St I) is a pore-forming toxin (PFT) produced by the Caribbean Sea anemone Stichodactyla helianthus belonging to actinoporin protein family, unique class of eukaryotic PFT. As for actinoporins, it has been proposed that presence cholesterol (Chol) and coexistence lipid phases increase binding target membrane ability. However, little known about role structure dynamics (phase state, fluidity, domains) on activity actinoporins or which regions are most favorable insertion,...
Abstract To investigate the role of N‐terminal region in lytic mechanism pore‐forming toxin sticholysin II (St II), we studied conformational and functional properties peptides encompassing first 30 residues protein. Peptides containing 1–30 (P1–30) 11–30 (P11–30) were synthesized their examined aqueous solution as a function peptide concentration, pH, ionic strength, addition secondary structure‐inducing solvent trifluoroethanol (TFE). CD spectra showed that increasing strength led to...
Crystallographic data of the dimeric and octameric forms fragaceatoxin C (FraC) suggested key role a small hydrophobic protein-protein interaction surface for actinoporins oligomerization pore formation in membranes. However, site-directed mutagenesis studies supporting this hypothesis others are still lacking. Here, we demonstrate that disrupting between V60 F163 (FraC numbering scheme) interface FraC, equinatoxin II (EqtII), sticholysin (StII) impairs activity these proteins. Our results...
Necroptosis is an inflammatory form of regulated cell death implicated in a range human pathologies, whose execution depends on the poorly understood pseudokinase mixed lineage kinase domain-like (MLKL). Here, we report that splicing-dependent insertion short amino acid sequence C-terminal α-helix (Hc) MLKL abolishes killing activity and creates anti-necroptotic isoform counteracts induced by necroptosis-proficient protein mice humans. We show interaction Hc with previously unrecognized...
Abstract The blockade of cellular differentiation represents a hallmark acute myeloid leukemia (AML), which is largely attributed to the dysfunction lineage-specific transcription factors controlling differentiation. However, alternative mechanisms programs in AML remain unexplored. Here we report that mixed lineage kinase domain-like protein (MLKL) contributes transformed hematopoietic progenitor cells AML. Using gene-targeted mice, show MLKL facilitates release granulocyte...