A5102990363- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Immunotherapy and Immune Responses
- Dental materials and restorations
- SARS-CoV-2 and COVID-19 Research
- Dental Implant Techniques and Outcomes
- COVID-19 Clinical Research Studies
- Pancreatic function and diabetes
- Corrosion Behavior and Inhibition
- Adipokines, Inflammation, and Metabolic Diseases
- Hydrogen embrittlement and corrosion behaviors in metals
- Bacteriophages and microbial interactions
- Heparin-Induced Thrombocytopenia and Thrombosis
- Anatomy and Medical Technology
- Immune cells in cancer
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 detection and testing
- Atherosclerosis and Cardiovascular Diseases
- Vaccine Coverage and Hesitancy
- Metabolism, Diabetes, and Cancer
- Engineering Technology and Methodologies
- vaccines and immunoinformatics approaches
- Geophysical and Geoelectrical Methods
- FOXO transcription factor regulation
Instituto Gulbenkian de Ciência
2009-2024
Umeå University
1993-2006
Karolinska Institutet
1980
Induction of Forkhead-box p3 (Foxp3) expression in developing T cells upon peptide-MHC encountering has been proposed to define a lineage committed Treg cells. However, sustained Foxp3 is required for function and what maintains peripheral remains obscure. To address this issue, we monitored natural phenotype adoptive transfer into lymphocyte-deficient mice. We first show that about 50% Foxp3-GFP(+) isolated from Foxp3(gfp) KI animals loose severe lymphopenic conditions. next evidence the...
The accumulation of adaptive mutations is essential for survival in novel environments. However, clonal populations with a high mutational supply, the power natural selection expected to be limited. This due interference - competition clones carrying different beneficial which leads loss many small effect and fixation large ones. If abundant, then mechanisms horizontal transfer genes, allow immediate combination alleles single background, are evolve. relevance complex environments, such as...
Understanding SARS-CoV-2 evolution and host immunity is critical to control COVID-19 pandemics. At the core an arms-race between antibody angiotensin-converting enzyme 2 (ACE2) recognition, a function of viral protein spike. Mutations in spike impacting and/or ACE2 binding are appearing worldwide, imposing need monitor SARS-CoV2 dynamics population. Determining signatures that render virus resistant neutralizing antibodies critical. We engineered 25 spike-pseudotyped lentiviruses containing...
Most Forkhead box P3 + (Foxp3 ) CD4 regulatory T cell (Treg) precursors are newly formed thymocytes that acquire Foxp3 expression on antigen encounter in the thymus. Differentiation of Treg, however, can also occur periphery. What limits this second layer self- and nonself-reactive Treg production physiological conditions remains to be understood. In work, we tested hypothesis that, similarly thymic peripheral immature cells. We show CD8 − recent emigrants (RTEs), contrarily naïve mature...
Abstract While mRNA vaccines are administrated worldwide in an effort to contain the COVID-19 pandemic, heterogeneity of humoral immune response they induce at population scale remains unclear. Here, a prospective, longitudinal, cohort-study, including 1245 hospital care workers and 146 nursing home residents scheduled for BNT162b2 vaccination, together covering adult ages from 19 99 years, we analyse seroconversion SARS-CoV-2 spike protein amount spike-specific IgG, IgM IgA before 3-5 weeks...
Abstract Regulatory T (TREG) cells develop via a program orchestrated by the transcription factor forkhead box protein P3 (FOXP3). Maintenance of TREG cell lineage relies on sustained FOXP3 mechanism involving demethylation cytosine-phosphate-guanine (CpG)-rich elements at conserved non-coding sequences (CNS) in locus. This cytosine is catalyzed ten–eleven translocation (TET) family dioxygenases, and it involves redox reaction that uses iron (Fe) as an essential cofactor. Here, we establish...
Defects in lymphocyte apoptosis may lead to autoimmune disorders and contribute the pathogenesis of type 1 diabetes. Lymphocytes nonobese diabetic (NOD) mice, an animal model diabetes, have been found resistant various signals, including alkylating drug cyclophosphamide. Using F2 intercross between apoptosis-resistant NOD mouse apoptosis-susceptible C57BL/6 mouse, we define a major locus controlling apoptosis-resistance phenotype demonstrate its linkage (logarithm odds score = 3.9) group...
Abstract A role for regulatory lymphocytes has been demonstrated in the pathogenesis of type 1 diabetes NOD mouse but nature these cells is debated. CD1d-restricted NKT have implicated this process. Previous reports reduced incidence mice which numbers are artificially increased attributed to enhanced production IL-4 by and a classical cells, using Vα14-Jα18 rearrangement. We now show that overexpression TCR Vα3.2+Vβ9+ producing high levels IFN-γ low amounts leads prevention diabetes,...
The experimental and analytical difficulties associated with the measurement of mercury vapor in oral cavity are considerable. In present paper, objective was to measure amount intra-oral subjects amalgams, by means two sets equipment based on different functional principles. addition, it found that type source prevalent had be evaluated. measuring technique used obtain correct results is discussed, an evaluation conditions for application available made. It released from time-dependent....
Specimens of four non-precious dental casting alloys intended for use in the ceramic-metal technique were implanted subcutaneously neck region mice. The animals killed after 5 months. Organs and tissues tested as regards concentration nickel by means atomic absorption spectrophotometry. study showed that due to electrochemical corrosion was released from implants accumulated some organs.
Nitric oxide (NO) and carbon monoxide (CO) are gasotransmitters that suppress the development of severe forms malaria associated with Plasmodium infection. Here, we addressed mechanism underlying their protective effect against experimental cerebral (ECM), a form develops in Plasmodium-infected mice, which resembles, many aspects, human (CM). NO suppresses pathogenesis ECM via involving (1) transcription factor nuclear erythroid 2-related 2 (NRF-2), (2) induction heme oxygenase-1 (HO-1), (3)...
Serological assays are valuable tools to study SARS-CoV-2 spread and, importantly, identify individuals that were already infected and would be potentially immune a virus reinfection. Spike protein its receptor binding domain (RBD) the antigens with higher potential develop serological assays. Moreover, structural studies of these key understand molecular basis for interaction angiotensin converting enzyme 2 receptor, hopefully enabling development COVID-19 therapeutics. Thus, it is urgent...
The NOD mouse is an important experimental model for human type 1 diabetes. T cells are central to pathogenesis, and their function in the autoimmune process of diabetes has been well studied. In contrast, although recognized as players disease induction, role B not clearly understood. this study we characterize different subpopulations demonstrate that marginal zone (MZ) expanded 2- 3-fold mice compared with nondiabetic C57BL/6 (B6) mice. MZ displayed a normal surface marker profile...
Galvanic currents within the oral cavity may have harmful effects on biological tissues. In present work 16 patients with different kinds of and other discomfort pain which they attributed to galvanism were investigated. The potential polarization each metal restoration reach a platinum probe measured versus reference electrode. A recording these values permits calculation pass between teeth. control group no subjective symptoms in was also results electrochemical measurements showed that...
The corrosion pattern of dental amalgam in aqueous media was interpreted theoretically by means log(α i /α ref )-pe diagrams. definitions on which the diagrams were based given, and their features described. All sparingly soluble compounds expected to be formed reactions with solvents considered listed. products reported current literature found formed, conditions for formation established. It emerged that it necessary exclude other might formed. Two compounds, CuSCN AgSCN, have not been...
Type 1 diabetes in the nonobese diabetic (NOD) mouse is a multifactorial and polygenic disease. The NOD-derived genetic factors that contribute to type are named Idd (insulin-dependent diabetes) loci. To date, biological functions of majority loci remain unknown. We have previously reported resistance NOD immature thymocytes depletion by dexamethazone (Dxm) maps Idd6 locus. Herein, we refine this phenotype using time-course experiment apoptosis induction upon Dxm treatment. confirm region...
Thymocytes differentiate into CD4(+) Foxp3(+) regulatory T cells (T(R)) upon interaction between their TCR and peptide-MHC II complexes locally expressed in the thymus. Conversion of naive T(R) can additionally take place periphery under noninflammatory conditions Ag encounter. In this study, making use transgenic models naturally devoid cells, we report de novo generation a single footpad injection mixed with classic proinflammatory adjuvant. Abrupt differentiation immunization occurred...
The authors have established a new immunodeficient mouse strain on the genetic background of diabetes prone non‐obese diabetic (NOD) mouse. A deletion mutant RAG‐2 gene was back crossed 10 generations onto NOD/Bom background. homozygous NOD rag−2−/− mice lack functionally mature B and T lymphocytes do not develop insulitis or throughout life. In contrast, heterozygous rag−2+/− both with an incidence similar to wild type mice. transfer experiments, spleen cells from donors were found disease...