A5009929705- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Plant Stress Responses and Tolerance
- Cancer therapeutics and mechanisms
- Carcinogens and Genotoxicity Assessment
- Redox biology and oxidative stress
- Drug Transport and Resistance Mechanisms
- Cancer-related Molecular Pathways
- Probiotics and Fermented Foods
- S100 Proteins and Annexins
- Carbohydrate Chemistry and Synthesis
- Synthesis and Characterization of Heterocyclic Compounds
- Lysosomal Storage Disorders Research
- Synthesis and Biological Evaluation
- Metal-Catalyzed Oxygenation Mechanisms
- Metal complexes synthesis and properties
- Biochemical and Structural Characterization
- Genetically Modified Organisms Research
- Enzyme function and inhibition
- Parasite Biology and Host Interactions
- Phosphodiesterase function and regulation
- PARP inhibition in cancer therapy
- Plant Molecular Biology Research
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2010-2024
Polish Academy of Sciences
2001-2012
University of Arizona
1995
Oxidative stress is involved in the pathogenesis of colon cancer. We wanted to elucidate at which stage disease this phenomenon occurs. In examined groups patients with colorectal cancer (CRC, n = 89), benign adenoma (AD, 77) and healthy volunteers (controls, 99), we measured: vitamins A, C E blood plasma, 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) 8-oxo-7,8-dihydroguanine (8-oxoGua) leukocytes urine, leukocyte 8-oxoGua excision activity, mRNA levels APE1, OGG1,...
Reactive oxygen species (ROS), play important roles in cellular signaling, nonetheless are toxic at higher concentrations. Cells have many interconnected, overlapped or backup systems to neutralize ROS, but their regulatory mechanisms remain poorly understood. Here, we reveal an essential role for mitochondrial AMPylase Fmp40 from budding yeast regulating the redox states of 1-Cys peroxiredoxin Prx1, which is only protein shown H
Expression of ZmCPK11 , a member the maize ( Zea mays ) calcium‐dependent protein kinases (CDPKs) family, is induced by mechanical wounding. A rapid increase activity 56‐kDa CDPK has been observed in damaged leaves. In present work, it shown that CDPK, identified as ZmCPK11, also activated non‐wounded leaves an element systemic wound response. Moreover, enzyme's and induction expression was after touching To study role touch signaling, transgenic Arabidopsis thaliana plants which...
The ethiology of colon cancer is largely dependent on inflammation driven oxidative stress. analysis 8-oxodeoxyguanosine (8-oxodGuo) level in leukocyte DNA healthy controls (138 individuals), patients with benign adenomas (AD, 137 individuals) and malignant carcinomas (CRC, 169 revealed a significant increase the 8-oxodGuo AD CRC comparison to controls. counteracting mechanism base excision repair, which OGG1 PARP-1 play key role. We investigated mRNA protein diseased marginal, normal...
In plants, CALCIUM-DEPENDENT PROTEIN KINASES (CDPKs/CPKs) are involved in calcium signaling response to endogenous and environmental stimuli. Here, we report that ZmCPK11, one of maize CDPKs, participates salt stress tolerance. Salt induced expression upregulated the activity ZmCPK11 roots leaves. Activation upon was also observed leaves transgenic Arabidopsis plants expressing ZmCPK11. The showed a long-root phenotype under control conditions short-root NaCl, abscisic acid (ABA) or jasmonic...
The chemically modified piezoelectrodes were utilized to develop relativelycheap and easy use biosensor for determination of genetically Roundup Readysoybean (RR soybean). relies on the immobilization onto goldpiezoelectrodes 21-mer single stranded oligonucleotide (probes) related to5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, which is an active componentof insert integrated into RR soybean genome. hybridization reaction between theprobe target complementary sequence in solution...
Gaucher disease (GD) is a rare inherited metabolic caused by pathogenic variants in the GBA1 gene. So far, pathomechanism of GD was investigated mainly animal models. In order to delineate molecular changes cells we analysed gene expression profile cultured skin fibroblasts from patients, control individuals and, additionally, patients with Niemann-Pick type C (NPC). We used microarrays subsequent validation qRT-PCR method. comparison vs. controls, most pronounced relative fold change (rFC)...
Calcium-dependent protein kinases (CDPKs or CPKs), unique to plants and some protists, are involved in growth developmental processes as well defence against diverse environmental stresses. CDPKs encoded by multi-gene families. Despite extensive studies of the many species, information about evolutionary history expression patterns CDPK family staple crop potato (Solanum tuberosum) remains poorly known. In this study, we performed bioinformatics analysis whole genome sequence identified 23...
The activity of arginase (ARGAH), which results in ornithine and urea production, is important for nitrogen metabolism all organisms as well defence responses. second‐stage juveniles the cyst‐forming nematode Heterodera schachtii penetrate roots induce formation a permanent feeding site. To determine whether infection with H. causes induction arginase, expression was studied Arabidopsis shoots at day inoculation 3, 7 15 days post‐inoculation (dpi). Parasite caused strong decrease root ARGAH1...
It was previously shown that 1,N 6-ethenoadenine (εA) in DNA rearranges into a pyrimidine ring-opened derivative of 20-fold higher mutagenic potency Escherichia coli (AB1157 lacΔU169) than the parental εA (Basu, A. K., Wood, M. L., Niedernhofer, L. J., Ramos, A., and Essigmann, J. (1993) Biochemistry 32, 12793–12801). We have found at pH 7.0, stability N-glycosidic bond εdA is lower dA. In alkaline conditions, but also neutrality, depurinates or converts products: → B C D. Compound product...
Abstract The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk hen feces) with respect their applicative potential colonize gastrointestinal track chickens before hatching an egg. In vitro phenotypic characterization lactobacilli included the investigation important prerequisites for persistence in tract, such as a capability survive presence bile salts at low pH , enzymatic sugar metabolic profiles, adhesion...
DNA glycosylases from the Fpg/Nei structural superfamily are base excision repair enzymes involved in removal of a wide variety mutagen and potentially lethal oxidized purines pyrimidines. Although genome stability, recent discovery synthetic relationships between other pathways highlights potential glycosylase inhibitors for future medicinal chemistry development cancer therapy. By combining biochemical approaches, physical target 2-thioxanthine (2TX), an uncompetitive inhibitor Fpg, was...
Background/Aim: Recently, we demonstrated the ability of inhibitors protein kinase 2 (casein II; CK2) to enhance efficacy 5-fluorouracil, a thymidylate synthase (TYMS)-directed drug for anticancer treatment. The present study aimed investigate antileukemic effect simultaneous inhibition dihydrofolate reductase (DHFR), another enzyme involved in biosynthesis cycle, and CK2 CCRF-CEM acute lymphoblastic leukemia cells. Materials Methods: influence combined treatment on apoptosis cell-cycle...
DNA base analogs, 2,4,5,6-substituted pyrimidines and 2,6-substituted purines were tested as potential inhibitors of E. coli Fpg protein (formamidopyrimidine -DNA glycosylase). Three the seventeen compounds revealed inhibitory properties. 2-Thioxanthine was most efficient, inhibiting 50% 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine (Fapy-7MeG) excision activity at 17.1 microM concentration. The measured K(i) 4.44 +/- 0.15 microM. Inhibition observed only when first challenged to its...
In a number of previous studies, our group has discovered an alternative pathway for lactose utilization in Lactococcus lactis that, addition to sugar-hydrolyzing enzyme with both P-β-glucosidase and P-β-galactosidase activity (BglS), engages chromosomally encoded components cellobiose-specific PTS (PTSCel-Lac), including PtcA, PtcB, CelB. this report, we show that system undergoes regulation via ClaR, novel activator protein from the RpiR family transcriptional regulators. Although proteins...
Abstract Reactive oxygen species (ROS), play important roles in cellular signaling, nonetheless are toxic at higher concentrations. Cells have many interconnected, overlapped or backup systems to neutralize ROS, but their regulatory mechanisms remain poorly understood. Here, we reveal an essential role for mitochondrial AMPylase Fmp40 from budding yeast regulating the redox states of 1-Cys peroxiredoxin, Prx1, which is only protein shown H 2 O with oxidation glutathione and Trx3,...
A folate analogue, 1843U89 (U89), with potential as a chemotherapeutic agent due to its potent and specific inhibition of thymidylate synthase (TS; EC 2.1.1.45), greatly enhances not only the binding 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP) dUMP Escherichia coli TS but also that dGMP, GMP, dIMP, IMP. Guanine nucleotide was first detected by CD analysis, which revealed unique spectrum for TS-dGMP-U89 ternary complex. The quantitative dGMP relative FdUMP, determined in presence...
To learn more about the signalling pathways involved in superoxide anion production guinea pig alveolar macrophages, triggered by Trichinella spiralis infection, protein level and phosphorylation of mitogen activated (MAP) kinases kinase C (PKC) were investigated. Infection with T. spiralis, nematode having 'lung phase' during colonization host, enhances PKC macrophages. Isoenzymes beta delta have been found significantly phosphorylated, although their location was not changed as a...
Oxidative stress and certain environmental carcinogens, e.g. vinyl chloride its metabolite chloroacetaldehyde (CAA), introduce promutagenic exocyclic adducts into DNA, among them 1,N(6)-ethenoadenine (epsilonA), 3,N(4)-ethenocytosine (epsilonC) N(2),3-ethenoguanine (epsilonG). We studied sequence-specific interaction of the vinyl-chloride CAA with human p53 gene exons 5-8, using DNA Polymerase Fingerprint Analysis (DPFA), identified sites highest sensitivity. CAA-induced damage was more...