A5026700968- Phytochemistry and Biological Activities
- Essential Oils and Antimicrobial Activity
- Immune Cell Function and Interaction
- Cancer therapeutics and mechanisms
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Sesquiterpenes and Asteraceae Studies
- Phytochemicals and Antioxidant Activities
- Synthesis and biological activity
- Natural product bioactivities and synthesis
- Angiogenesis and VEGF in Cancer
- Biological Activity of Diterpenoids and Biflavonoids
- Phytochemistry and Bioactive Compounds
- Psoriasis: Treatment and Pathogenesis
- Antiplatelet Therapy and Cardiovascular Diseases
- Molecular Biology Techniques and Applications
- Metal complexes synthesis and properties
- Building energy efficiency and sustainability
- Bee Products Chemical Analysis
- Sphingolipid Metabolism and Signaling
- Cytomegalovirus and herpesvirus research
- Advances in Cucurbitaceae Research
- Whipple's Disease and Interleukins
- Synthesis of heterocyclic compounds
- Mesenchymal stem cell research
Medical University of Warsaw
2015-2024
Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) is of critical importance for reliable mRNA expression analysis. Due to a diversity putative reference genes stability different vitro models, validation an internal control gene should be made each particular tissue or cell type and every specific experimental design. A few approaches have been proposed selection, including pair-wise comparison approach model-based approach. In this...
Creating a functional vascularized bone tissue remains one of the main goals engineering. Recently, growing interest in crosstalk between endothelial cells (EC) and osteoblasts (OB), two players new formation, has been observed. However, only few reports have addressed mutual influence OB EC on cell proliferation. Our study focuses this issue by investigating cocultures human bone-derived (HBDC) umbilical vein (HUVEC). Three various proportions used that is, HBDC:HUVEC 1:1, 1:4, 4:1 were...
Aim of the study: Taking into account that overactivated leukocytes are an important factor in development many chronic diseases, we investigated activity phytochemically characterized (HPLC-DAD-MSn) extracts from forsythia leaves and flowers on pro- anti-inflammatory functions (effects IL-1β, IL-8, TNF-α, TGFβ release) their adherence to endothelial cells. Using bio-guided fractionation, isolated active compounds determined biological activity, included positive control quercetin. Methods:...
Abstract A continuing quest for specific inhibitors of proinflammatory cytokines brings promise effective therapies designed inflammatory and autoimmune disorders. Cefazolin, a safe, first-generation cephalosporin antibiotic, has been recently shown to specifically interact with interleukin 15 (IL-15) receptor subunit α (IL-15Rα) inhibit IL-15-dependent TNF-α IL-17 synthesis. The aim this study was elucidate cefazolin activity against IL-2, IL-4, IL-15 IL-21, i.e. four sharing the common...
Penta‐ O ‐galloyl‐β‐D‐glucose (PGG) occurrs in high concentrations medicinal herbs such as Rhus chinensis , Paeonia suffruticosa Acer truncatum and Terminalia chebula which demonstrate anti‐inflammatory activity. We investigated the effect of PGG on stimulated non‐stimulated neutrophils processes included reactive oxygen species generation (ROS), metalloproteinase‐9 interleukin‐8 secretion (IL‐8), β 2 integrin (CD11b) L‐selectin (CD62L) expression apoptosis. In 5 μM–20 μM, demonstrated...
During chronic inflammation, neutrophils acting locally as effector cells not only activate antibacterial defense but also promote the inflammatory response. Interleukin 8 (IL-8), main cytokine produced by activated neutrophils, positively correlates with severity of respiratory tract diseases. By screening European plants traditionally used for treating diseases, we found that extracts aerial parts Eupatorium cannabinum inhibit IL-8 release from neutrophils. Using bioassay-guided...
The effectiveness of opioids in the treatment neuropathic pain is limited. It was demonstrated that magnesium ions (Mg2+), physiological antagonists N-methyl-D-aspartate receptor (NMDAR), increase opioid analgesia chronic pain. Our study aimed to determine molecular mechanism this action. Early data indicate cross-regulation µ (MOR) and NMDAR control. Morphine acting on MOR stimulates protein kinase C (PKC), while induction recruits A (PKA), leading a disruption MOR-NMDAR complex promoting...
Combretastatins isolated from the Combretum caffrum tree belong to a group of closely related stilbenes. They are colchicine binding site inhibitors which disrupt polymerization process microtubules in tubulins, causing mitotic arrest. In vitro and vivo studies have proven that some combretastatins exhibit antitumor properties, among them, combretastatin A-4 is most active inhibitor. this study, series novel analogs containing carboxylic acid, ester, amide moieties were synthesized their...
During forced degradation, the intrinsic stability of active pharmaceutical ingredients (APIs) could be determined and possible impurities that would occur during shelf life drug substance or product estimated. Vildagliptin belongs to relatively new oral antidiabetic drugs named gliptins, inhibiting dipeptidyl peptidase 4 (DPP-4) prolonging activities endogenous incretin hormones. At same time, some gliptins were shown as prone degradation under specific pH temperature conditions, well in...
Abstract New halogenated thiourea derivatives were synthesized via the reaction of substituted phenylisothiocyanates with aromatic amines. Their cytotoxic activity was examined in vitro studies against solid tumors (SW480, SW620, PC3), a hematological malignance (K‐562), and normal keratinocytes (HaCaT). Most compounds more effective SW480 ( 1a , 3a 3b 5j ), K‐562 2b 4a or PC3 5d ) cells than cisplatin, favorable selectivity. anticancer mechanisms studied by Annexin...
Chemical stability is one of the main problems during discovery and development potent drugs. When ignored, it may lead to unreliable biological pharmacokinetics data, especially regarding degradation products' possible toxicity. Recently, two biologically active drug candidates were presented that combine both opioid neurotensin pharmacophores in entity, thus generating a hybrid compound. Importantly, these chimeras are structurally similar except for an amino acid change at position 9...
Polygoni avicularis herba is a pharmacopoeial medical material used for upper respiratory tract disorders and as diuretic agent. It contains flavonoids dominating group of compounds. Previous studies have shown that besides some well-known compounds such avicularin, isoquercitrin hyperoside, extracts from contain significant quantities several flavonol glucuronides which presence not been confirmed before in this plant (1).
Tragopogon tommasinii Sch.Bip. is a perennial plant with large yellow flowering heads, endemic to the Illyrian floristic region. Previous phytochemical studies taxa yielded flavonoids, phenolic acids, sterols, triterpenes, triterpene glycosides, and saponins.
Medicinal plants and food products are a rich source of polyphenols. One the largest group compounds ingested with plant flavonoids. Flavonoid glycosides after oral intake hydrolysed in digestive system absorbed form free aglycones. When aglycones reach circulation they undergo second phase metabolism liver kidneys. They conjugated glucuronic acid or sulphate can be methylated by catechol-O-methyltransferase. Mainly these forms circulate blood stream to next eliminated urine [1,2]. The aim...