A5078642523- Bacterial Infections and Vaccines
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Polar Research and Ecology
- Machine Learning in Bioinformatics
- Collembola Taxonomy and Ecology Studies
- RNA and protein synthesis mechanisms
- Insect and Arachnid Ecology and Behavior
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- vaccines and immunoinformatics approaches
- Biochemical and Structural Characterization
- Gender, Labor, and Family Dynamics
- Antibiotic Resistance in Bacteria
- Immunodeficiency and Autoimmune Disorders
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Marine Biology and Ecology Research
- Family Dynamics and Relationships
- Intergenerational Family Dynamics and Caregiving
Toscana Life Sciences
2020-2021
R2M Solution (Italy)
2016
Novartis (Italy)
2010-2015
Novartis (Switzerland)
2014
University of Siena
2009-2013
Newron Pharmaceuticals (Italy)
2013
Allan Wilson Centre
2009
Massey University
2009
Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance these bacteria. We have determined genome sequence ExPEC IHE3034 (ST95) isolated from case neonatal meningitis compared this available sequences other strains few nonpathogenic E. . found 19 genomic islands present IHE3034, which absent isolates. By using subtractive reverse vaccinology...
ABSTRACT The ability to adhere and adapt the human respiratory tract mucosa plays a pivotal role in pathogenic lifestyle of nontypeable Haemophilus influenzae (NTHi). However, temporal events associated with successful colonization have not been fully characterized. In this study, by reconstituting ciliated bronchial epithelium vitro , we monitored global transcriptional changes NTHi infected mucosal simultaneously for up 72 h dual RNA sequencing. initial stage was characterized binding...
Abstract Aim We examined the genetic structure among populations and regions for springtails Cryptopygus antarcticus Gomphiocephalus hodgsoni (Collembola) to identify potential historical refugia subsequent colonization routes, examine population growth/expansion relative ages of divergence. Location Antarctic Peninsula C. a. ; continent (southern Victoria Land) G. . Methods Samples were collected from 24 28 locations across southern Land , respectively. used genetic, demographic nested...
The 4CMenB vaccine licensed against serogroup B Neisseria meningitidis (MenB) contains three recombinant proteins and Outer Membrane Vesicles (OMV) from a New Zealand epidemic strain. protective response mediated on differentmeningococcal strains has been historically ascribed to one of the four main antigens fHbp, NHBA, NadA, PorA nominated as immunodominant antigen OMV component. It is however accepted that extensive cross-protection observed after vaccination may be attributed other in...
Gomphiocephalus hodgsoni Carpenter was only the second collembolon † to be described from Antarctic continent. It collected first in 1902 Granite Harbour, southern Victoria Land, Eastern Antarctica, by British National Expedition (1901–1904). Since then several studies have investigated distribution, ecology, ecophysiology and molecular composition of species. Despite two morphological redescriptions an absence detailed evolutionary phylogenetic studies, genus recently reduced a subgenus...
Abstract Streptococcus agalactiae (Group B , GBS) causes life-threatening infections in newborns and adults with chronic medical conditions. Serotype IV strains are emerging both among carriers as cause of invasive disease recent studies revealed two main Sequence Types (STs), ST-452 ST-459 assigned to Clonal Complexes CC23 CC1, respectively. Whole genome sequencing 70 type GBS subsequent phylogenetic analysis elucidated the localization isolates a SNP-based tree suggested that could have...
The Neisseria meningitidis (Nm) chromosome shows a high abundance of simple sequence DNA repeats (SSRs) that undergo stochastic, reversible mutations at frequency. This mechanism is reflected in an extensive phenotypic diversity facilitates Nm adaptation to dynamic environmental changes. To date, phase-variable phenotypes mediated by SSRs variation have been experimentally confirmed for 26 genes. Here we present population-scale comparative genomic analysis identified 277 genes and...
Abstract Friesea grisea is the only springtail species currently described from both East (Victoria Land) and West Antarctica (Antarctic Peninsula), although levels of genetic divergence between two regions suggest possibility cryptic species. Determining structure populations in necessary order to compare effects different environmental conditions regions, evolutionary histories their inhabitants, for assessing any influence latitude each region on diversity. We analysed sequences...
Abstract The mitochondrial COI gene of the Antarctic springtail, Gressittacantha terranova , was sequenced across a polar coastal landscape at Terra Nova Bay, northern Victoria Land. Samples from two altitudinal transects in foothills directly south Campbell Glacier were compared with samples Springtail Valley (northern foothills) as an external reference population. We found that mtDNA haplotypes clustered into lineages (clades) mean sequence divergence 10% (uncorrected distance). However,...
Multi-Locus Sequence Typing (MLST) of Streptococcus pneumoniae is based on the sequence seven housekeeping gene fragments. The analysis MLST allelic profiles by eBURST allows grouping genetically related strains into Clonal Complexes (CCs) including those genotypes with a common descent from predicted ancestor. However, increasing use to characterize S. has led identification large number new Types (STs) causing merger formerly distinct lineages larger CCs. An example this CC156, displaying...
Abstract Serogroup B meningococcus (MenB) is a leading cause of meningitis and sepsis across the world vaccination most effective way to protect against this disease. 4CMenB multi-component vaccine MenB, which now licensed for use in subjects >2 months age several countries. In study, we describe development an ad hoc protein microarray study immune response induced by three major antigenic components (fHbp, NHBA NadA) individual sera from vaccinated infants, adolescents adults. The...
Haemophilus influenzae is an important human pathogen involved in invasive disease. Here, we report the whole-genome sequences of 11 nonencapsulated H. (ncHi) strains isolated from both disease and healthy carriers Italy. This genomic information will enrich our understanding molecular basis ncHi pathogenesis.
Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory illness in children less than 5 years age which usually results hospitalization or even death. Vaccine development hampered consequence a failed vaccine trial with fatalities 1960s. Even though research has been more focused on RSV fusion protein its pre-fusion conformation, maternal vaccination post-fusion (post F) was considered as promising strategy for passive immunization babies, because post F preserves...
ABSTRACT Gonococcus (Gc), a bacterium resistant to most antibiotics causing more than 80 million cases of gonorrhea annually, is WHO high priority pathogen. Recently, vaccine development prospects were boosted by reports that licensed meningococcus serogroup B (MenB) vaccines provided partial protection against Gc infection. To determine antigens responsible for cross-protection, memory cells from 4CMenB vaccinated volunteers single-cell sorted identify antibodies kill in bactericidal assay....
Respiratory syncytial virus (RSV) is the primary cause for acute lower respiratory syndrome in children younger than 5 years. Research on B cell repertoires and antibodies binding RSV fusion protein (RSV F) of major interest development potential vaccine candidates therapies. receptors (BCRs) which have higher affinities a specific antigen are preferentially selected clonal expansion germinal center reactions. Consequently, antigen-specific BCR share common features, as instance preferential...