A5080664355- Meat and Animal Product Quality
- Animal Nutrition and Physiology
- Effects of Environmental Stressors on Livestock
- Genetic and phenotypic traits in livestock
- Adipose Tissue and Metabolism
- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- Pharmacological Effects and Assays
- Calpain Protease Function and Regulation
- Birth, Development, and Health
- Muscle Physiology and Disorders
- Metabolism, Diabetes, and Cancer
- Economic and Environmental Valuation
- Animal Behavior and Welfare Studies
- Protein Hydrolysis and Bioactive Peptides
- Bee Products Chemical Analysis
- Organic Food and Agriculture
- Urban Stormwater Management Solutions
- Sensory Analysis and Statistical Methods
- Fatty Acid Research and Health
- Coccidia and coccidiosis research
- Agriculture Sustainability and Environmental Impact
- Microbial Inactivation Methods
- Metal and Thin Film Mechanics
- Salmonella and Campylobacter epidemiology
South Dakota State University
2016-2025
University of Wyoming
2006-2011
University of Illinois Urbana-Champaign
2006
Maternal obesity and over-nutrition give rise to both obstetric problems neonatal morbidity. The objective of this study was evaluate effects maternal on signalling the AMP-activated protein kinase (AMPK) pathway in fetal skeletal muscle an obese pregnant sheep model. Non-pregnant ewes were assigned a control group (Con, fed 100% NRC nutrient recommendations, n = 7) or obesogenic (OB, 150% National Research Council (NRC) diet from 60 days before 75 after conception (term 150 days) when...
Angus × Gelbvieh cows with 2 to 3 previous pregnancies were used evaluate effects of maternal nutrient restriction on offspring adipose tissue morphology at standard production endpoints. At 45 d after AI a single sire, pregnancy was confirmed and randomly allotted into groups fed control (Con, 100% NRC recommendations), nutrient-restricted (NR, 70% Con diet), or + protein-supplemented (NRP, essential AA supply the small intestine equal Con) diet. 185 gestation, commingled received diet...
Abstract The objective of this study was to characterize the influence finishing system on sensory characteristics and mechanisms tenderness formation in longissimus lumborum (striploin) bison bulls. Bison bulls (n = 196) were randomly assigned one two treatments at approximately 25 mo age: 1) Grain-finished 98; placed an open lot with ad libitum access prairie hay, alfalfa whole shell corn prior slaughter) or 2) Grass-finished allowed graze native pasture until slaughter). Bulls harvested...
Abstract Stocker cattle can be managed in many grazing environments (variable range health, forage quality and quantity), which could impact subsequent performance carcass attributes. The objective of this research was to determine the influence vegetative composition diversity driven by history during stocker phase on traits economic returns beef steers. Yearling steers (n = 134, 116, 131, 2020, 2021, 2022, respectively) were assigned randomly pastures that had been at three levels stocking...
Marbling, or i.m. fat, is an important factor determining beef quality. Both adipogenesis and hypertrophy of existing adipocytes contribute to enhanced marbling. We hypothesized that the fetal stage for formation AMP-activated protein kinase (AMPK) has a key role in during this stage. The objective study was assess AMPK sheep muscle 3T3-L1 cells. Nonpregnant ewes were randomly assigned control (Con, 100% NRC recommendations, n = 7) overfed (OF, 150% NRC, diet from 60 d before 75 after...
Research has suggested that maternal undernutrition may cause the development of a thrifty phenotype in offspring, potentially resulting greater adiposity and reduced muscle mass. These alterations adipose could have lasting impacts on offspring growth, carcass characteristics, meat quality. However, limited research exists evaluating impact energy status these economically important traits offspring. Therefore, objective this study was to determine influence during midgestation...
Pale, soft, and exudative (PSE) meat has been recognized for decades. Fast glycolysis during early post-mortem stage while the muscle temperature is still high cause of PSE meat. To elucidate molecular mechanism underlying this fast in to become meat, ATP metabolism, fructose-2,6-diphosphate content, activities AMPK, glycogen phosphorylase, pyruvate kinase were examined muscle. Earlier faster AMPK activation was responsible significantly lower pH higher lactic acid accumulation (p < 0.05)...
Abstract The National Beef Quality Audit (NBQA)-2022 serves as a benchmark of the current market cow and bull sectors U.S. beef industry allows comparison to previous audits method monitoring progress. From September 2021 through May 2022, livestock trailers (n = 125), live animals 5,430), post-slaughter hide-on 6,674) were surveyed at 20 commercial processing facilities across Cattle transported in variety trailer types for an average distance 490.6 km mean transport time 6.3 h. During...
The presence of the halothane gene results in PSE meat. However, exact mechanisms linking and incidence meat remain unclear. We hypothesize that accelerates energy consumption postmortem muscle, which activates adenosine monophosphate-activated protein kinase (AMPK), leading to enhanced glycolysis To test our hypothesis, status, AMPK activity, LM carrier halothane-negative pigs were compared. showed accelerated depletion muscle immediately after exsanguination, rapid early ATP, as shown by...
Postmortem proteolysis is an important determinant of beef tenderness. Caspase 3 a protease that functions in apoptosis and has been shown to degrade myofibrillar proteins. Our objective was evaluate whether caspase activity related tenderness muscle growth, activated postmortem muscle. In experiment 1, longissimus thoracis (LT) sternomandibularis samples were obtained at 0, 0.25, 3, 24, 72, 240 h from 5 steers. 2, group 40 cattle slaughtered the University Wyoming Meat Lab with 10 steers...
Tenderness is a key palatability trait influencing perception of consumers meat quality and influenced by multitude factors, including postmortem proteolysis. A fundamental understanding this biological mechanism regulating tenderness necessary to decrease variability increase consumer satisfaction. However, reports regarding the enzyme systems involved in tenderization are conflicting. Therefore, objective study was determine if caspase-3 responsible for degradation myofibrillar proteins...
The objectives of this study were to 1) determine the conditions (temperature and pH) that exist in early postmortem muscle normally chilled delay beef carcasses provide a model for vitro work 2) mechanism by which temperature/pH found influence enzymes regulate aging process vitro. For objective 1, 7 finished animals (HCW 385 ± 8 kg) harvested with right sides subjected normal chilling (2.3°C) approximately 1.25 h left ambient temperature (delay chilling; 22.6°C) an additional 4.75 then...
The objective of this study was to determine whether altered maternal energy supply during mid-gestation results in differences muscle histology or genes regulating fetal adipose and development. In total, 22 Angus cross-bred heifers (BW=527.73±8.3 kg) were assigned randomly the three dietary treatments providing 146% (HIGH; n=7), 87% (INT; n=7) 72% (LOW; n=8) requirements for from day 85 180 gestation. Fetuses removed via cesarean section at gestation longissimus (LM) subcutaneous fat...
The objective of this study was to examine the association adenosine monophosphate (AMP)-activated protein kinase (AMPK) with glycogen content in bovine muscle and their links intramuscular fat (IMF) fiber type composition. Five steers high (High IMF, IMF is 5.71 ± 0.36%) five low (Low 2.09 0.19%) longissimus thoracis (LM) were selected for immunoblotting, glycogen, myofiber composition analyses. higher Low than High (1.07 0.07 versus 0.85 0.08 g/100 g muscle, P < 0.05). Phosphorylation AMPK...
This study examined consumers’ preferences and willingness to pay (WTP) for locally produced ground beef with an emphasis on the impacts of product attributes heterogeneity caused by rural characteristics. We applied choice-based conjoint technique analyze data collected from a typical town in Northern Great Plains. The results indicate that location associated brand differences leanness were dominant components affecting WTP. premium consumers close tie local production is $0.48/lb. Cut...
Electrical stimulation (ES) is used in beef slaughter plants to improve tenderness; however, varying levels of low-voltage ES have not been well characterized. The objective was evaluate the influence two on temperature decline, pH, glycolytic potential, and meat quality. Forty-two carcasses were chosen from a commercial packing facility. One side each carcass received either 40 or 80 volts for 60 s at 45 min postmortem. paired did receive (Control). Temperature loggers placed sirloin 12...
Subcutaneous fat and marbling both increase in beef cattle during the feeding phase but are antagonistic regard to their contribution carcass value. The objective of this study was determine whether cellular factors associated with development change growth stage throughout period they correlated relative composition. Twenty-four steers known origin cytosine thymine (CT) leptin genotype were allotted 3 harvest groups. Six per group harvested at following predetermined points: 35 d on feed...
Abstract The objective of this experiment was to evaluate effects postruminal flows casein or glutamic acid on small intestinal starch digestion and quantify changes in energy nutrient balance. Twenty-four steers (body weight = 179 ± 4 kg) were duodenally infused with raw cornstarch (1.46 0.04 kg/d) either 413 7.0 g casein/d, 121 3.6 acid/d water (control). Measures excretion collected across d after 42 infusion measures respiration via indirect calorimetry 2 48 infusion. Ileal flow least...