A5031649617- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Chemokine receptors and signaling
- Lymphoma Diagnosis and Treatment
- Vitamin D Research Studies
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- interferon and immune responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tuberculosis Research and Epidemiology
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Brain Metastases and Treatment
- Vitamin C and Antioxidants Research
- Immunodeficiency and Autoimmune Disorders
Hospitais da Universidade de Coimbra
2020
University of Coimbra
2010-2014
Centro de Neurociências e Biologia Celular
2013
Objective CD8+ T cells are abundant in rheumatoid arthritis (RA). However, their role disease pathogenesis is poorly defined. This study was undertaken to investigate the relationship between activity and cell phenotypes, production of cytokines, cytotoxic molecules peripheral blood (PB) synovial fluid (SF) patients with RA. Methods phenotypes were determined 96 RA (44 remission, 34 active disease, 18 low activity) 64 sex‐ age‐matched healthy controls. Ten paired PB SF samples from analyzed....
Glioblastoma (GBM) is the most aggressive and malignant brain tumor. Recent studies indicated that glioma samples are characterized by increased expression of CXCR4, CXCL12/SDF-1 chemokine receptor. To better understand role CXCR4 in GBM biology we performed an integrated study where simultaneously evaluate contribution CXCR4/CXCL12 signaling pathway to proliferation, survival motility a human cell line. Our results axis induced increase proliferation motility. The blockage significant...
Glioblastomas (GBM) are the most lethal subtype of astrocytomas, with a mean patient survival rate 12 months after diagnosis. The gold standard treatment GBM, which includes surgery followed by combination radiotherapy and chemotherapy temozolomide (TMZ), increases to 14.6 months. success TMZ appears be limited occurrence chemoresistance that allows glioma cells escape from death signaling pathways. However, mechanism action is yet clarified although some controversial results have been...
Background Colitis is a common clinical complication in chronic granulomatous disease (CGD), primary immunodeficiency caused by impaired oxidative burst. Existing experimental data from NADPH-oxidase knockout mice propose contradictory roles for the involvement of reactive oxygen species colitis chronicity and severity. Since genetically controlled with point-mutation Ncf1 gene are susceptible to inflammation autoimmunity, we tested whether they presented increased predisposition develop...
CD8+ T cells are part of the cell pool infiltrating synovium in rheumatoid arthritis (RA). However, their role pathogenesis RA has not been fully delineated. Using K/BxN mouse model spontaneous chronic arthritis, which shares many similarities with RA, we studied potential depletion monoclonal antibodies (mAb) to stop and reverse progression experimental arthritis.CD8+ from blood articular infiltrate mice were characterized for surface phenotypic markers cytokine production. Additionally,...
Natural killer (NK) cells play a crucial role in the innate immune response. In absence of prior activation they are capable rapidly produce interferon γ (IFNγ) and other immunoregulatory cytokines, as well lyse specific target cells. Previous studies suggested dual for NK rheumatoid arthritis (RA) limited their characterisation to surface expression CD56/CD16. However, newest information on heterogeneity complexity this cell population has deemed such classification insufficient. an effort...
<h3>Background and Objectives</h3> CD8<sup>+</sup>T cells are known to be present in the synovial fluid synovium of rheumatoid arthritis. However, their role disease pathogenesis remains largely unexplored. This work intended characterise blood cell functional subsets found patients with arthritis explore importance regulating activity disease. <h3>Methods</h3> The phenotypes were determined seventy-eight (forty-four remission (DAS28 < 2.6), thirty-four active > 3.2)) results compared...
<h3>Background and objectives</h3> Detailed information on CD8<sup>+</sup> T cells in rheumatoid arthritis (RA) is still reduced. However, studies animal models of from the authors9 team others suggest a major potential role pathogenesis chronic polyarthritis. In present study authors characterised phenotype cytokine-production profile peripheral blood (PB) synovial fluid (SF) RA patients. <h3>Materials methods</h3> Unstimulated PB SF 40 patients with established were analysed by...
Natural killer (NK) cells play a crucial role in the innate immune response. In absence of prior activation they are capable rapidly produce interferon γ (IFNγ) and other immunoregulatory cytokines, as well lyse specific target cells. Previous studies suggested dual for NK rheumatoid arthritis (RA) limited their characterisation to surface expression CD56/CD16. However, newest information on heterogeneity complexity this cell population has deemed such classification insufficient. an effort...
<h3>Background</h3> For the past years there has been a clear increase in interest for T cells autoimmune diseases (<i>1</i>), where it was found that CD8 can have effector or regulatory functions (<i>2,3</i>). Similar findings rheumatoid arthritis, indicate may either pro-inflammatory (<i>4</i>) an anti-inflammatory function mouse models of inflammatory arthritis (<i>5</i>). One argue these results be result two different cell subtypes with enhancing and suppressor down-regulating disease....
Gliomas are brain tumours that account for more than 50% of the arise within central nervous system. They highly proliferative, angiogenic, and develop resistance to alkylating agents used in chemotherapy. The median survival time glioma patients remains approximately 12-14 months treated with temozolomide (TMZ) which is considered main chemotherapeutic agent. In order understand cellular mechanisms involved chemoresistance cells we analysed activation autophagy TMZ. addition, determined...