A5066888086- Vibrio bacteria research studies
- SARS-CoV-2 and COVID-19 Research
- Zebrafish Biomedical Research Applications
- Legionella and Acanthamoeba research
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Aquaculture disease management and microbiota
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Bacterial Infections and Vaccines
- Mathematical and Theoretical Epidemiology and Ecology Models
- Long-Term Effects of COVID-19
- Cell Image Analysis Techniques
- Retinal and Optic Conditions
- Muscle Physiology and Disorders
- 3D Printing in Biomedical Research
- Influenza Virus Research Studies
Institut Pasteur
2019-2024
Centre National de la Recherche Scientifique
2019-2023
Laboratoire de Biologie du Développement
2023
Biologie du Développement et Cellules Souches
2023
Université Paris Cité
2023
The evolution of the IFN system, major innate antiviral mechanism vertebrates, remains poorly understood. According to detection type I genes in cartilaginous fish genomes, system appeared 500 My ago. However, integrates many other components, most which are encoded by IFN-stimulated (ISGs). To shed light on its evolution, we have used deep RNA sequencing generate a comprehensive list ISGs zebrafish, taking advantage high-quality genome annotation this species. We analyzed larvae after...
The pathogenic bacterium Streptococcus pneumoniae (S. pneumoniae) can invade the cerebrospinal fluid (CSF) and cause meningitis with devastating consequences. Whether how sensory cells in central nervous system (CNS) become activated during bacterial infection, as recently reported for peripheral system, is not known. We find that CSF infection by S. larval zebrafish leads to changes posture behavior are reminiscent of pneumococcal meningitis, including dorsal arching epileptic-like...
Animal models are essential to understanding COVID-19 pathophysiology and for preclinical assessment of drugs other therapeutic or prophylactic interventions. We explored the small, cheap, transparent zebrafish larva as a potential host SARS-CoV-2. Bath exposure, well microinjection in coelom, pericardium, brain ventricle, bloodstream, resulted rapid decrease SARS-CoV-2 RNA wild-type larvae. However, when virus was inoculated swim bladder, viral stabilized after 24 h. By...
Adult skeletal muscle is capable of active and efficient differentiation in the event injury both physiological pathological conditions, such as Duchenne muscular dystrophy (DMD). DMD characterized by different features, continuous cycles degeneration/regeneration, fiber heterogeneity, chronic inflammation fibrosis. A well-defined standardized approach for histological morphometric analysis samples necessary order to measure quantify specific regenerative parameters myopathies. Indeed,...
Abstract Animal models are essential to understand COVID-19 pathophysiology and for pre-clinical assessment of drugs other therapeutic or prophylactic interventions. We explored the small, cheap transparent zebrafish larva as a potential host SARS-CoV-2. Bath exposure, well microinjection in coelom, pericardium, brain ventricle, bloodstream, yolk, did not result detectable SARS-CoV-2 replication wild-type larvae. However, when virus was inoculated swim bladder, modest increase viral RNA...
The zebrafish has become a powerful model organism to study host-pathogen interactions. Here, we developed dissect the innate immune response Legionella pneumophila during infection. We show that L . cause larvae death in dose dependent manner. Additionally, macrophages are first line of defence and cooperate with neutrophils clear Immunocompromised humans have an increased propensity develop pneumonia, similarly, when either or depleted, these “immunocompromised” lethally sensitive Also, as...
Abstract Organ-to-organ dissemination of viruses is a critical feature host-virus interactions. In particular, neuroinvasive are able to enter the central nervous systems (CNS), which may result in death or permanent neurological impairment. The complex mechanisms underpinning this spread poorly understood, as they depend on variety parameters, including initial site entry, route access CNS, and immune responses. To better understand these phenomena, we analyzed spatial dynamics Sindbis...
Abstract The evolution of the interferon (IFN) system, major innate antiviral mechanism vertebrates, remains poorly understood. According to detection type I IFN genes in cartilaginous fish genomes, system appeared 500My ago. However, integrates many other components, most which are encoded by IFN-stimulated (ISGs). To shed light on its evolution, we have used deep RNA sequencing generate a comprehensive list ISGs zebrafish, taking advantage high quality genome annotation this species. We...
Abstract The zebrafish has become a powerful model organism to study host-pathogen interactions. Here, we developed of Legionella pneumophila infection dissect innate immune responses. We show that L. cause larvae death in dose dependent manner, and macrophages are the first line defence, with neutrophils cooperating clear infection. When either or depleted, these “immunocompromised” lethally sensitive similar what is known for humans develop pneumonia. Also as observed human infections,...
ABSTRACT Biological research is in constant need of new methodological developments to assess organization and functions at various scales ranging from whole organisms interactions between proteins. One the main ways evidence quantify biological phenomena imaging. Fluorescence microscopy label-free are particular highly active fields due their compatibility with living samples as well versatility. The Imabio Young Scientists Network (YSN) a group young scientists (PhD students, postdocs...