A5079857755- Zebrafish Biomedical Research Applications
- Advanced Fluorescence Microscopy Techniques
- Respiratory viral infections research
- Molecular Biology Techniques and Applications
- Pulmonary Hypertension Research and Treatments
- Reproductive Biology and Fertility
- Cellular transport and secretion
- Animal Virus Infections Studies
- Congenital Diaphragmatic Hernia Studies
- Cytokine Signaling Pathways and Interactions
- Microtubule and mitosis dynamics
- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 and COVID-19 Research
- Metabolism, Diabetes, and Cancer
- Fish Ecology and Management Studies
- Protein Kinase Regulation and GTPase Signaling
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Virus-based gene therapy research
- Aquaculture disease management and microbiota
- Muscle Physiology and Disorders
- Characterization and Applications of Magnetic Nanoparticles
- Bioactive Compounds and Antitumor Agents
- Molecular Communication and Nanonetworks
Université Paris-Saclay
2019-2025
Virologie et Immunologie Moléculaires
2019-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université de Versailles Saint-Quentin-en-Yvelines
2020-2024
Fish Physiology and Genomics Institute
2015-2019
Centre National de la Recherche Scientifique
2009-2014
Institut Pasteur
2012-2014
The beneficial contribution of commensal bacteria to host health and homeostasis led the concept that exogenous non-pathogenic called probiotics could be used limit disease caused by pathogens. However, despite recent progress using gnotobiotic mammal invertebrate models, mechanisms underlying protection afforded probiotic against pathogens remain poorly understood. Here we developed a zebrafish model controlled co-infection in which germ-free raised on axenic living protozoa enabled study...
Abstract The olfactory mucosa, where the first step of odor detection occurs, is a privileged pathway for environmental toxicants and pathogens toward central nervous system. Indeed, some can infect sensory neurons including their axons projecting to bulb allowing them bypass blood–brain barrier reach system (CNS) through so‐called pathway. respiratory syncytial virus (RSV) major tract pathogen but there growing evidence that RSV may lead CNS impairments. However, mechanisms involved in...
Animal models are essential to understanding COVID-19 pathophysiology and for preclinical assessment of drugs other therapeutic or prophylactic interventions. We explored the small, cheap, transparent zebrafish larva as a potential host SARS-CoV-2. Bath exposure, well microinjection in coelom, pericardium, brain ventricle, bloodstream, resulted rapid decrease SARS-CoV-2 RNA wild-type larvae. However, when virus was inoculated swim bladder, viral stabilized after 24 h. By...
Abstract Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates encompasses multiple applications in applied basic research. Many studies have focused on 2D images, which is time consuming implies extrapolation results. Three-dimensional imaging whole organs recently became an important challenge to better understand their architecture allow cell enumeration. Several protocols thus been developed enhance sample transparency, limiting...
Swine lymph nodes (LN) present an inverted structure compared to mouse and human, with the afferent diffusing from centre periphery. This structure, also observed in close distant species such as dolphins, hippopotamus, rhinoceros elephants, is poorly described, nor are LN macrophage populations their relationship B cell follicles. maturation occurs mainly follicles help of endowed different antigen delivery capacities. We identified three that we localized spatial organization. allowed us...
Hereditary hemorrhagic Telangiectasia (HHT) is a rare genetic disease caused by mutations affecting components of Bone Morphogenetic Protein and Transforming Growth Factor-β (BMP/TGF-β) signaling in endothelial cells. This disorder characterized arterio-venous malformations which are prone to rupture the ensuing hemorrhages responsible for iron deficiency anemia. Along with Activin receptor-like kinase ALK1, ENDOGLIN associated vast majority HHT cases. In this report, we zebrafish endoglin...
The porcine reproductive and respiratory syndrome virus (PRRSV), an RNA inducing abortion in sows disease young pigs, is a leading infectious cause of economic losses the swine industry. Modified live vaccines (MLVs) help controlling disease, but their efficacy often compromised by high genetic diversity circulating viruses, to vaccine escape variants field. In this study, we hypothesized that DNA prime with naked plasmids encoding PRRSV antigens containing conserved T-cell epitopes may...
This investigation used primary cultured rat vascular smooth muscle cells (VSMCs) to examine the effect of insulin (INS) on proliferation VSMCs. In this study, we investigated role protein kinase B (Akt) and p42/44 mitogen-activated (ERK 1/2) signaling pathways in mediating mitogenic action INS Incubation VSMCs with (100 nM) for 10 min resulted an increase Akt phosphorylation by 6-fold (p<0.001) ERK 1/2 3-fold (p<0.001). Pretreatment 15 μM PI3K/Akt inhibitor LY294002 or 20 PD98059, 1/2,...
Abstract The dramatic increase in myotomal muscle mass teleosts appears to be related their sustained ability produce new fibres the growing muscle. To describe fibre input dynamics trout (Oncorhynchus mykiss), we generated a stable transgenic line carrying green fluorescent protein (GFP) cDNA driven by myogenin promoter. In this myog:GFP line, cell recruitment is revealed appearance of fluorescent, small, nascent fibres. displayed formation patterns developing and showed that production...
Abstract Animal models are essential to understand COVID-19 pathophysiology and for pre-clinical assessment of drugs other therapeutic or prophylactic interventions. We explored the small, cheap transparent zebrafish larva as a potential host SARS-CoV-2. Bath exposure, well microinjection in coelom, pericardium, brain ventricle, bloodstream, yolk, did not result detectable SARS-CoV-2 replication wild-type larvae. However, when virus was inoculated swim bladder, modest increase viral RNA...
Abstract Insulin (INS) via INS receptor acts as a mitogen in vascular smooth muscle cells (VSMCs) through stimulation of multiple signaling mechanisms, including p42/44 mitogen‐activated protein kinase (ERK1/2) and phosphatidyl inositol‐3 (PI3K). In addition, cytosolic phospholipase 2 (cPLA ) is linked to VSMCs proliferation. However, the upstream mechanisms responsible for activation cPLA are not well defined. Therefore, this investigation used primary cultured rat examine role PI3K ERK1/2...
Background: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for elderly immune-compromised people. In absence vaccines or effective treatments, better characterization pathogenesis RSV infection required. To date, pathophysiology disease its diagnosis has mostly relied on chest X-ray genome detection nasopharyngeal swabs. The development new imaging approaches instrumental to further description...
An in situ screen for genes expressed the skeletal muscle of eyed-stage trout embryos led to identification a transcript encoding polypeptide related CILP1, secreted glycoprotein present extracellular matrix.In hybridisation developing revealed that CILP1 expression was initially detected fast progenitors early somite.Later, down-regulated medio-laterally differentiating cells, become finally restricted undifferentiated forming dermomyotomelike epithelium at surface embryonic myotome.At...
In zebrafish, lymphatic endothelial cells (LECs) originate from multiple/several distinct progenitor populations and generate organ-specific vasculatures. Cell fate tissue specificities were determined using a combination of genetically engineered transgenic lines in which the promoter LEC-specific gene drives expression fluorescent reporter protein. We established novel zebrafish line expressing eGFP under control part batf3 (Basic Leucine Zipper ATF-Like Transcription Factor 3)....
Cargo transport by molecular motors along microtubules is essential for the function of eukaryotic cells, in particular neurons which axonal defects constitute early pathological features neurodegenerative diseases. Mainly studied motor and sensory neurons, still difficult to characterize brain absence appropriate vivo tools. Here, we measured fast tracing second harmonic generation (SHG) signal potassium titanyl phosphate (KTP) nanocrystals (nanoKTP) endocytosed zebrafish (Zf) larvae....
Abstract Overactive inflammation is directly correlated with airway damage and early death in individuals cystic fibrosis (CF), a genetic disorder caused by mutation the CFTR gene. Reducing impact of inflammatory therefore major concern CF. Several studies indicate that decrease nuclear factor erythroid 2-related factor-2 (NRF2) signaling people CF may hamper their ability to alleviate oxidative stress inflammation, although role NRF2 has not been determined. Therefore, we examined whether...
Spring viremia of carp virus (SVCV) has a broad fish host spectrum and is responsible for disease that generally affects juvenile fishes with mortality rate up to 90%. In the absence treatments or vaccines against SVCV, search prophylactic therapeutic solutions thus relevant, particularly identify compatible mass vaccination. addition being threat aquaculture ecosystems, SVCV unique pathogen study virus-host interactions in zebrafish model. Establishing first reverse genetics system design...
Abstract Background Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for elderly immune-compromised people. In absence vaccines or effective treatments, better characterization pathogenesis RSV infection required. To date, pathophysiology disease its diagnosis mostly relied on chest x-ray genome detection nasopharyngeal swabs. The development new imaging approaches instrumental to further...