A5038177834- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Hepatitis Viruses Studies and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Veterinary medicine and infectious diseases
- Prion Diseases and Protein Misfolding
- T-cell and Retrovirus Studies
- Molecular Biology Techniques and Applications
- Zoonotic diseases and public health
- Viral Infections and Vectors
- HIV Research and Treatment
- Circular RNAs in diseases
- Plant Virus Research Studies
- MicroRNA in disease regulation
- interferon and immune responses
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2016-2025
Advanced Network and Services (United States)
2024
CEA Paris-Saclay
2020-2022
Virologie et Immunologie Moléculaires
2022
Université Bretagne Loire
2016-2019
Institut du Porc
2018-2019
Université Européenne de Bretagne
2014-2015
Pathophysiology, Diagnosis and Treatment of Bone Diseases
2004
Inserm
2004
École Pratique des Hautes Études
2004
In Europe, modified live vaccines (MLV) are commonly used to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, they have been associated with safety issues such as reversion virulence induced by mutation and/or recombination. On a French pig farm, we identified field recombinant strain derived from two PRRSV-1 MLV (MLV1). As result, aimed evaluate its clinical, virological, transmission parameters in comparison both parental strains. Three groups six...
Abstract Background Porcine reproductive and respiratory syndrome (PRRS) is a viral disease with worldwide distribution an enormous economic impact. To control PRRS virus (PRRSV) infection, modified live vaccines (MLVs) are widely used in the field, mainly administered via intramuscular (IM) route. Currently, some MLVs authorized for intradermal (ID) administration, which has many practical welfare advantages. The objectives of study were to compare immune responses (systemic blood mucosal...
Porcine reproductive and respiratory syndrome virus (PRRSV) replicates primarily in pulmonary alveolar macrophages (PAMs) the resulting lung damage is influenced by strain virulence. To better understand pathogenesis of PRRSV infection, we performed a longitudinal study PAM population cytokines specific pathogen-free pigs infected either with highly pathogenic Lena or low Finistere comparison to uninfected pigs. Bronchoalveolar lavage fluid (BALF) blood were collected follow viral, cellular...
<title>Abstract</title> Farmed pigs are frequently exposed to respiratory infections, with swine influenza A virus (swIAV) and porcine reproductive syndrome (PRRSV) being key drivers. Most co-infection studies these viruses have focused on PRRSV infection followed by swIAV. However, the reverse scenario, where swIAV is given first then PRRSV, has not been explored. This sequence plausible under natural conditions warrants further study, especially that shown in mice impair alveolar...
In developed countries, most of hepatitis E human cases are zoonotic origin. Swine is a major virus (HEV) reservoir and foodborne transmissions after pork product consumption have been described. The risk for HEV-containing pig livers at slaughter time related to the age infection shedding duration. Porcine Reproductive Respiratory Syndrome Virus (PRRSV) that impairs immune response; it highly prevalent in production areas suspected influence HEV dynamics. impact PRRSV on features infections...
Abstract The conventional C-strain vaccine induces early protection against classical swine fever (CSF), but infected animals cannot be distinguished from vaccinated animals. CP7_E2alf marker vaccine, a pestivirus chimera, could suitable substitute for to control CSF outbreaks. In this study, single oral applications of and vaccines were compared their efficacy induce virus (CSFV) challenge with the moderately virulent Bas-Rhin isolate, in pigs as two days post-immunization. This work...
Lung inflammation is frequently involved in respiratory conditions and it strongly controlled by mononuclear phagocytes (MNP). We previously studied porcine lung MNP described a new population of cells presenting all the features alveolar macrophages (AM) except for their parenchymal location, that we named AM-like cells. Herein showed are phagocytosing blood-borne particles, agreement with pulmonary intravascular (PIM) identity. PIM have been microscopically long time ago species from...
Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is an arterivirus responsible for highly contagious infection huge economic losses in pig industry. Two PRRSV genotypes have been identified: the genotype 1 from European origin 2 North American origin. Genotypes further divided into four subtypes. Genotype subtype 3 (type 1.3) such as Lena are more pathogenic than type 1.1 Lelystad or Flanders13. One main feature of all strains their capacity to delay appearance neutralizing...
Classical swine fever (CSF) severity is dependent on the virulence of CSF virus (CSFV) strain.The earliest event detected following CSFV infection a decrease in lymphocytes number.With some strains this leads to lymphopenia, varying according strain virulence.This lymphocyte depletion attributed an induction apoptosis non-infected bystander cells.We collected peripheral blood mononuclear cells (PBMC) before and during 3 days post-infection with either highly or moderately virulent subjected...
This paper provides information on the complete genome sequence of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated French pig farm which was identified as recombinant from two commercial modified live vaccine strains genotype 1 (VP-046BIS DV strains).
Swine lymph nodes (LN) present an inverted structure compared to mouse and human, with the afferent diffusing from centre periphery. This structure, also observed in close distant species such as dolphins, hippopotamus, rhinoceros elephants, is poorly described, nor are LN macrophage populations their relationship B cell follicles. maturation occurs mainly follicles help of endowed different antigen delivery capacities. We identified three that we localized spatial organization. allowed us...
Respiratory infections are still a major concern in pigs. Amongst the involved viruses, porcine reproductive and respiratory syndrome virus (PRRSV) swine influenza type A (swIAV) have impact. These viruses frequently encounter dual reported. We analyzed here molecular interactions between tracheal epithelial cells as well lung tissue. PRRSV-1 species do not infect cells. However, PRRSV-1, when inoculated simultaneously or shortly before swIAV, was able to inhibit swIAV H1N2 infection,...
Abstract Host miRNAs are known to modulate the cell response virus infections. We characterized miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). performed immunoprecipitation RISC (RNA-induced Silencing Complex) followed by microarray analysis RISC-bound miRNA targets (RIP-Chip) evaluate relative enrichment or depletion expressed genes in RISC. The...
Modified-live vaccines (MLVs) against porcine reproductive and respiratory syndrome viruses (PRRSVs) are usually administrated to piglets at weaning when swine influenza A virus (swIAV) infections frequently occur. SwIAV infection induces a strong interferon alpha (IFNa) response IFNa was shown abrogate PRRSV2 MLV replication an inherent immune response. In this study, we evaluated the impacts of swIAV on PRRSV1 (MLV1), post-vaccine responses post-challenge vaccine efficacy both systemic...
Porcine reproductive and respiratory syndrome virus (PRRSV) swine influenza A (swIAV) are major pathogens of the porcine disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological immunological outcomes successive infections with PRRSV-1 H1N2 swIAV. Twenty-four specific pathogen-free piglets were distributed into four groups inoculated either PRRSV at study day (SD) 0, or swIAV SD8, one week apart SD0 respectively,...