A5006585346- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Ion-surface interactions and analysis
- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- Electrohydrodynamics and Fluid Dynamics
- Advanced Polymer Synthesis and Characterization
- Redox biology and oxidative stress
- Advanced Chemical Sensor Technologies
- Photoreceptor and optogenetics research
- Polymer crystallization and properties
- Nanocluster Synthesis and Applications
- Advanced Chemical Physics Studies
- Inorganic Chemistry and Materials
- Block Copolymer Self-Assembly
- Sulfur Compounds in Biology
- Fuel Cells and Related Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bacterial Identification and Susceptibility Testing
- Protein Structure and Dynamics
- Porphyrin and Phthalocyanine Chemistry
- Protein Interaction Studies and Fluorescence Analysis
- Inorganic Fluorides and Related Compounds
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2013-2024
Université Claude Bernard Lyon 1
2012-2022
Centre National de la Recherche Scientifique
2012-2022
Institut des Sciences Analytiques
2012-2022
École Normale Supérieure de Lyon
2016-2018
Université de Lyon
2014
Laboratoire de Spectrométrie Ionique et Moléculaire
2011-2012
Purdue University West Lafayette
2010
Aix-Marseille Université
2006-2009
Laboratoire de Chimie Bactérienne
2009
Functional group derivatization reactions occur in the course of microdroplet/surface collisions ambient ionization process desorption electrospray (DESI). The unique environment microdroplet causes rate enhancements as much several orders magnitude typical bimolecular that proceed through either cationic or anionic intermediates. evaporating charged differs from bulk: (i) pH solution moves towards extremes, (ii) concentrations reagents increase, (iii) relative surface area increases and...
The present paper describes the successful one-pot synthesis of self-stabilized particles composed amphiphilic block copolymers based on poly(methacrylic acid) (PMAA) obtained by polymerization-induced self-assembly. First, controlled radical polymerization MAA is performed in water using RAFT process taking advantage our recent results showing [ChaducMacromolecules 2012, 45, 1241−1247]. so-formed hydrophilic macroRAFT agents are then chain-extended situ with a hydrophobic monomer to form...
For the first time, electrospray ionization efficiency (IE) scales in positive and negative mode are united into a single system enabling direct comparison of IE values across modes. This is made possible by use reference compound that ionizes to similar extent both Thus, choosing optimal (i.e., most sensitive) conditions for given set analytes enabled. Ionization efficiencies 33 compounds ionizing modes demonstrate that, contrary general practice, allows better sensitivity 46% such whereas...
Allelic polymorphism of the apolipoprotein E (ApoE) gene (ApoE ε2, ApoE ε3 and ε4 alleles) gives rise to three protein isoforms (ApoE2, ApoE3 ApoE4) that differ by 1 or 2 amino acids. Inheritance allele is a risk factor for developing Alzheimer's disease (AD). The potential diagnostic value levels in biological fluids (i.e. cerebrospinal fluid, plasma serum) distinguishing between AD patients healthy elderly subjects subject great controversy. Although recent study reported subnormal total...
Desorption electrospray ionization (DESI) mass spectrometry (MS) is used in an imaging mode to interrogate the lipid profiles of 15 μm thin tissue cross sections injured rat spinal cord and normal healthy tissue. Increased relative intensities fatty acids, diacylglycerols, lysolipids (between +120% +240%) as well a small decrease lipids (−30%) were visualized lesion epicenter adjacent areas after injury. This indicates hydrolysis during demyelination process due activation phospholipase A2...
Abstract Metalloenzymes preorganize the reaction environment to steer substrate(s) along required coordinate. Here, we show that phosphine ligands selectively facilitate protonation of binuclear silver hydride cations, [LAg 2 (H)] + by optimizing geometry active site. This is a key step in selective, catalysed extrusion carbon dioxide from formic acid, HO CH, with important applications (for example, hydrogen storage). Gas-phase ion-molecule reactions, collision-induced dissociation (CID),...
Electrospray (ESI) ionization efficiencies (IE) of a set 10 compounds differing by chemical nature, extent in solution (basicity), and hydrophobicity (tetrapropylammonium tetraethylammonium ion, triethylamine, 1-naphthylamine, N,N-dimethylaniline, diphenylphthalate, dimethylphtahalate, piperidine, pyrrolidine, pyridine) have been measured seven mobile phases (three acetonitrile percentages 20%, 50%, 80%, three different pH-adjusting additives, 0.1% formic acid, 1 mM ammonia, pH 5.0 buffer...
Imaging mass spectrometry allows for the direct investigation of tissue samples to identify specific biological compounds and determine their spatial distributions. Desorption electrospray ionization (DESI) has been used imaging analysis rat spinal cord cross sections. Glycerophospholipids sphingolipids, as well fatty acids, were detected in both negative positive ion modes identified through tandem (MS/MS) product scans using collision-induced dissociation accurate measurements. Differences...
We investigated how the temperature and size of charged droplets are affected by electrospray ionization (ESI) process, using in situ measurements involving laser-induced fluorescence Mie scattering on a thermal gradient focusing ESI source. Rhodamine dyes were employed as indicators ratiometric intensity-based techniques. The results compared to lifetime-based techniques tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate, [Ru(bpy)3](2+). Both methods gave similar profiles. Nevertheless,...
Herein we report the successful implementation of consecutive and simultaneous photodissociation with high (213 nm) low (10.6 μm) energy photons (HiLoPD, high-low photodissociation) on ubiquitin in a quadrupole-Orbitrap mass spectrometer. Absorption high-energy UV photon is dispersed over whole protein stimulates extensive C–Cα backbone fragmentation, whereas low-energy IR gradually increases internal thus preferentially dissociates most labile amide (C–N) bonds. We noticed that irradiation...
UV photodissociation of proline-containing peptide ions leads to unusual product ions. In this paper, we report laser-induced dissociation a series peptides at 213 nm. We observe specific fragmentation pathways corresponding the formation (y-2), (a + 2) and (b fragment This was not observed 266 nm or for which do contain proline residues. order obtain insights into dynamics nm, small (RPK arginine-proline-lysine) studied both theoretically experimentally. Calculations absorption spectra...
Mass-spectrometry-based methods have made significant progress in the characterization of post-translational modifications (PTMs) peptides and proteins; however, room remains to improve fragmentation methods. Ideal MS/MS are expected simultaneously provide extensive sequence information localization PTM sites retain labile groups. This collection criteria is difficult meet, various activation available today offer different capabilities. In order examine specific case phosphorylation on...
Electrospray ionization tandem mass spectrometry has been used to characterize the microstructure of a nitroxide-mediated poly(ethylene oxide)/polystyrene block copolymer, called SG1-capped PEO-b-PS. The main dissociation route co-oligomers adducted with lithium or silver cation was observed proceed via homolytic cleavage C-ON bond, aimed at undergoing reversible homolysis during nitroxide mediated polymerization. This results in elimination terminal SG1 end-group as radical, inducing...
We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profiling setup conjunction with fast, high-resolution particle sizing scheme on modified Agilent Jet Stream source coupled to single quadrupole analyser. The optical permits us profile solvent...
Abstract Coupling of liquid chromatography at critical conditions (LCCC) with on‐line mass spectrometry (MS) detection was implemented via an electrospray ionization (ESI) interface, using a mobile phase containing the cationizing agent. Critical established for poly(ethylene oxide) were used to characterize oxide)/polystyrene block copolymer (PEO‐ b ‐PS) in both MS and MS/MS modes. As co‐oligomer molecules successfully separated according PS size, structural information could be reached...
The role of cations in the fragmentation polymer nitroxide labile end groups, as often deplored matrix-assisted laser desorption/ionization, was investigated. A combination different spectroscopic techniques and theoretical calculations implemented to address mechanistic aspect end-group cleavage a nitroxide-terminated poly(ethylene oxide) macroinitiator agent upon activation mass spectrometry. When occurring during ionization process, homolytic C−ON bond group results release moiety...
For the first time, quantitative electrospray (ESI) ionization efficiencies (IE), expressed as logIE values, obtained on different mass-spectrometric setups (four mass analyzers and four ESI sources) are compared for 15 compounds of diverse properties. The general trends change IE with molecular structure same all experimental setups. scales could be applied setups: there were no statistically significant changes in order efficiency root mean squared differences values between compiled...
Nanoparticle-based temperature imaging is an emerging field of advanced applications. Herein, the sensitivity fluorescence rhodamine B-doped latex nanoparticles toward described. Submicrometer size particles were prepared by a surfactant-free emulsion polymerization method that allowed simple and inexpensive way to incorporate B into nanoparticles. Also, B-coated dispersed in water order address effect dye location on their dependence. A better linearity dependence emission B-embedded...
Mass spectrometry based methods have made significant progress in characterizing post-translational modifications peptides and proteins; however, certain aspects regarding fragmentation must still be improved.A good technique is expected to provide excellent sequence information, locate PTM sites retain the labile groups.To address these issues, we investigate 10.6 μm IRMPD, 213 nm UVPD combined UV IR photodissociation, known as HiLoPD (High-Low Photodissociation), for phospho-, sulfo-and...
Atomically precise, ligand-protected gold nanoclusters (AuNCs) attract considerable attention as contrast agents in the biosensing field. However, control of their optical properties and functionalization surface ligands remain challenging. Here we report a strategy to tailor AuNCs for precise detection protein carbonylation-a causal biomarker ageing. We produce Au15SG13 (SG glutathione) with atomic precision functionalize it thiolated aminooxy moiety impart carbonyl-binding properties. Mass...
Multistage mass spectrometry and density functional theory (DFT) were used to characterise the small silver hydride nanocluster, [Ag3 H2 L](+) (where L=(Ph2 P)2 CH2 ) its gas-phase unimolecular chemistry. Collision-induced dissociation (CID) yields [Ag2 HL](+) as major product while laser-induced (LID) proceeds via formation subsequent release from , giving rise product. Deuterium labelling studies on D2 prove that source of is hydrides not ligand. Comparison TD-DFT absorption patterns...
Characterization of acidic peptides and proteins is greatly hindered due to lack suitable analytical techniques. Here we present the implementation 213 nm ultraviolet photodissociation (UVPD) in high-resolution quadrupole-Orbitrap mass spectrometer negative polarity for peptide anions. Radical-driven backbone fragmentation provides 22 distinctive fragment ion types, achieving complete sequence coverage all reported peptides. Hydrogen-deficient radical anion not only promotes cleavage Cα-C...