A5047162260- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Iron Metabolism and Disorders
- Blood groups and transfusion
- Hemoglobin structure and function
- Blood properties and coagulation
- Peroxisome Proliferator-Activated Receptors
- Metabolism and Genetic Disorders
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Hematopoietic Stem Cell Transplantation
- Phagocytosis and Immune Regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Bone and Joint Diseases
- Malaria Research and Control
- Nitric Oxide and Endothelin Effects
- Microfluidic and Bio-sensing Technologies
- Erythropoietin and Anemia Treatment
- Antioxidant Activity and Oxidative Stress
- Sphingolipid Metabolism and Signaling
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- Folate and B Vitamins Research
Universidad Católica de Santa Fe
2024
University of California, San Francisco
1999-2024
UCSF Benioff Children's Hospital
2020-2022
Oakland University
1991-2016
California State University, East Bay
2005
University of Pittsburgh
2003
Purdue University West Lafayette
2002
Lawrence Berkeley National Laboratory
1994-2001
Albert Einstein College of Medicine
2001
National Institutes of Health
2001
Pulmonary hypertension is a life-threatening complication of sickle cell disease. L-Arginine the nitrogen donor for synthesis nitric oxide, potent vasodilator that deficient during times crisis. This deficiency may play role in pulmonary hypertension. The enzyme arginase hydrolyzes arginine to ornithine and urea, thus, it compete with oxide synthase, leading decreased production. Nitric therapy by inhalation has improved associated acute chest syndrome disease, several studies demonstrate...
Artemisinin is an important new antimalarial agent containing a bridged endoperoxide. The in vitro activity of artemisinin derivative, arteether, antagonized by two iron chelators, pyridoxal benzoylhydrazone and 1,2-dimethyl-3-hydroxypyrid-4-one. Similarly, the acute toxicity mice another chelator, deferoxamine-hydroxyethylstarch. A combination hemin oxidizes erythrocyte membrane thiols vitro, this oxidation also inhibited chelator. Thus, plays role mechanisms action artemisinin. decreases...
Recent studies suggest that a nitric oxide (NO) deficiency and elevated arginase activity may play role in the pathogenesis of asthma. Although much attention has been directed toward measurements exhaled NO asthma, no to date have evaluated levels plasma or arginine, substrate for production, patients with This study, therefore, measured amino acid levels, activity, metabolites blood as well breath. virtually all acids were reduced, asthma exhibited striking reduction arginine compared...
Purpose Our objective was to evaluate l-arginine and nitric oxide metabolite (NOx) levels in children with sickle cell disease (SCD) at steady-state during vaso-occlusive crisis (VOC). Because alterations production may have an important role the pathophysiology of SCD, our second aim determine if a relationship exists between these Patients Methods Plasma serum NOx were examined 36 patients SCD 39 episodes VOC 10 steady-state. Daily obtained requiring hospitalization. Results Steady-state...
Painful episodes of vaso-occlusion are the leading cause hospitalizations and emergency department visits in sickle cell disease, associated with increased mortality. Low nitric oxide bioavailability contributes to vasculopathy disease. Since arginine is obligate substrate for production, an acute deficiency pain, we hypothesized that may be a beneficial treatment pain related Thirty-eight children disease hospitalized 56 were randomized into this double-blinded placebo-controlled trial....
Abstract Nine inbred strains of mice, which are progenitors recombinant sets, were evaluated for aortic lesion formation and plasma liver lipid levels. This survey was done to determine if a semi‐synthetic high‐fat diet could elicit the same extent diet‐induced atherosclerosis as that observed in mice fed natural ingredient highfat discover strain‐specific variants future genetic characterization. Evaluation lesions after 18 wk consumption showed C57BL/6J, C57L/J, SWR/J SM/J susceptible A/J,...
By consensus, the acyl-CoA synthetase (ACS) community, with advice of human and mouse genome nomenclature committees, has revised for mammalian long-chain synthetases. ACS is family root name, genes ACSs are termed ACSL1,3-6 Acsl1,3-6, respectively. Splice variants ACSL3, -4, -5, -6 cataloged. Suggestions naming other members nonmammalian synthetases made.
Abstract The pathogenesis of pulmonary hypertension (PAH), a serious complication in thalassemia, is not well understood. Thromboembolism has been postulated as one the causative factors; however, there are currently limited specific data on its role. To examine whether increased platelet activation and hypercoagulability linked to PAH, 25 β‐thalassemia major intermedia patients were evaluated with Doppler echocardiograms for estimation artery pressure laboratory assays indications...
The mammalian RBC lacks de novo lipid synthesis but maintains its membrane composition by rapid turnover of acyl moieties at the sn-2 position phospholipids. Plasma-derived fatty acids are esterified to acyl-CoA synthetases and transferred lysophospholipids acyl-CoA:lysophospholipid acyltransferases. We report characterization three lysophosphatidylcholine (lysoPC) acyltransferases (LPCATs), products AYTL1, -2, -3 genes. These proteins members a LPCAT family, which all genes expressed in an...
Summary Sickle cell disease (SCD) is characterized by progressive vascular injury and its pathophysiology strikingly similar to that of atherosclerosis. Statins decrease inflammation improve endothelial function in cardiovascular disease, but their effect SCD not known. In this pilot study, we examined the safety short‐term simvastatin on biomarkers dysfunction SCD. We treated 26 patients with simvastatin, 20 or 40 mg/d, for 21 d. Plasma nitric oxide metabolites (NOx), C‐reactive protein...