A5038764569- Listeria monocytogenes in Food Safety
- Gut microbiota and health
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Biosensors and Analytical Detection
- Advancements in Transdermal Drug Delivery
- Dermatology and Skin Diseases
- Essential Oils and Antimicrobial Activity
- Food Allergy and Anaphylaxis Research
- Food composition and properties
- Clostridium difficile and Clostridium perfringens research
- Acoustic Wave Resonator Technologies
- Microbial Inactivation Methods
- Wound Healing and Treatments
- Advanced Chemical Sensor Technologies
- Proteins in Food Systems
- Barrier Structure and Function Studies
- Viral gastroenteritis research and epidemiology
- Trace Elements in Health
- Antimicrobial Peptides and Activities
Purdue University West Lafayette
2021-2025
ABSTRACT The cellular junctional architecture remodeling by Listeria adhesion protein-heat shock protein 60 (LAP-Hsp60) interaction for monocytogenes ( Lm ) passage through the epithelial barrier is incompletely understood. Here, using gerbil model, permissive to internalin (Inl) A/B-mediated pathways like in humans, we demonstrate that crosses intestinal villi at 48 h post-infection. In contrast, single isogenic lap − or Δ inlA double mutant strains show significant defects. LAP promotes...
Contamination of meat with pathogenic microorganisms can cause severe illnesses and food waste, which has significant negative impacts on both general health the economy. In many cases, expiration date is not a good indicator freshness as there high risk contamination during handling throughout supply chain. Many biomarkers, including color, odor, pH, temperature, volatile compounds, are used to determine spoilage. Among these, pH presents simple effective biomarker directly linked...
Abstract Bacterial wound infections are a significant socioeconomic concern in the modern healthcare industry owing to increased morbidity, prolonged hospital stay, and mortality. infectious agents that colonize bed develop biofilms, acting as physical barrier prevents effective penetration of topical antimicrobials. Further, bacteria such wounds express wide range virulence factors promoting intercellular transmigration host cell invasion complicating treatment regimen. To address this...
Environmental cues promote microbial biofilm formation and physiological genetic heterogeneity. In food production facilities, biofilms produced by pathogens are a major source for contamination; however, the pathogenesis of biofilm-isolated sessile cells is not well understood. We investigated Listeria monocytogenes (Lm) using cell culture mouse models. Lm express reduced levels lap, inlA, hly, prfA, sigB show adhesion, invasion, translocation, cytotoxicity in model than planktonic cells....
Listeria adhesion protein (LAP) is a secreted acetaldehyde alcohol dehydrogenase (AdhE) that anchors to an unknown molecule on the monocytogenes (Lm) surface, which critical for its intestinal epithelium crossing. In present work, immunoprecipitation and mass spectrometry identify internalin B (InlB) as primary ligand of LAP (KD ∼ 42 nM). InlB-deleted naturally InlB-deficient Lm strains show reduced LAP-InlB interaction LAP-mediated pathology in murine intestine brain invasion....
Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community improve gastrointestinal health. Biofilm formation, antimicrobial activities, immunological responses of probiotics crucial improving gut Thus, we tested a commercial probiotic blend (LabMAX-3), canine kibble additive comprising Lactobacillus acidophilus, Lacticasibacillus casei, Enterococcus faecium for their ability inactivate...
Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community improve gastrointestinal health. Biofilm formation, antimicrobial activities, immunological responses of probiotics crucial improving gut Thus, we tested a commercial probiotic blend (LabMAX-3), canine kibble additive comprising
ABSTRACT The cellular junctional architecture remodeling by LAP-Hsp60 interaction for L. monocytogenes ( Lm ) passage through the epithelial barrier is incompletely understood. Here, using gerbil model, permissive to internalin (Inl) A/B-mediated pathways like in humans, we demonstrate that crosses intestinal villi at 48 h post-infection. In contrast, single isogenic lap ─ or Δ inlA double ΔinlA mutant strains show significant defects. LAP promotes translocation via endocytosis of cell-cell...