A5016780885- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Microbial bioremediation and biosurfactants
- Arsenic contamination and mitigation
- Planetary Science and Exploration
- Selenium in Biological Systems
- Radioactive contamination and transfer
- Radioactive element chemistry and processing
- Astro and Planetary Science
- Analytical chemistry methods development
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Chemical Analysis and Environmental Impact
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Global Energy and Sustainability Research
- Laser-Ablation Synthesis of Nanoparticles
- Isotope Analysis in Ecology
- Protist diversity and phylogeny
- Water Treatment and Disinfection
- Nonlinear Optical Materials Studies
- Chemical Reactions and Isotopes
- Poisoning and overdose treatments
- Phase-change materials and chalcogenides
- Spaceflight effects on biology
Florida International University
2023
United States Geological Survey
2009-2023
University of California, Berkeley
2023
Karlsruhe Institute of Technology
2023
University of Alabama
2023
Ben-Gurion University of the Negev
2023
Duquesne University
2005
ABSTRACT Certain toxic elements support the metabolism of diverse prokaryotes by serving as respiratory electron acceptors for growth. Here, we demonstrate that two anaerobes previously shown to be capable respiring oxyanions selenium also achieve growth reduction either tellurate [Te(VI)] or tellurite [Te(IV)] elemental tellurium [Te(0)]. This achieves a sizeable stable-Te-isotopic fractionation (isotopic enrichment factor [ε] = −0.4 −1.0 per ml atomic mass unit) and results in formation...
Searles Lake is a salt-saturated, alkaline brine unusually rich in the toxic element arsenic. Arsenic speciation changed from arsenate [As(V)] to arsenite [As(III)] with sediment depth. Incubated anoxic slurries displayed dissimilatory As(V)-reductase activity that was markedly stimulated by H2 or sulfide, whereas aerobic had rapid As(III)-oxidase activity. An anaerobic, extremely haloalkaliphilic bacterium isolated grew via As(V) respiration, using either lactate sulfide as its electron...
Elementary tellurium is currently of great interest as an element with potential promise in nano-technology applications because the recent discovery regarding its three two-dimensional phases and existence Weyl nodes around Femi level. Here, we report on unique nano-photonic properties elemental particles [Te(0)], harvest from a culture tellurium-oxyanion respiring bacteria. The bacterially-formed nano-crystals prove effective photonic tested compared to chemically-formed nano-materials,...
Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation trichloroethene (TCE) by situ minerals, and C2H2 is known to inhibit bacterial dechlorination. In this study, we show that while high (1.3 mM) concentrations reversibly reductive dechlorination TCE Dehalococcoides mccartyi isolates well enrichment cultures containing D. sp., low (0.4 do not growth or metabolism mccartyi. Cocultures Pelobacter SFB93, C2H2-fermenting bacterium, with...
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting mixture terrestrial and marine emissions from anthropogenic, biomass-burning, unidentified biogenic sources. Fermentation acetylene was serendipitously discovered during C2H2 block assays N2O reductase, Pelobacter acetylenicus shown to grow on via hydratase (AH). AH W-containing, catabolic, low-redox-potential enzyme that, unlike nitrogenase (N2ase), specific for acetylene. fermentation rare...
Anoxic samples (sediment and groundwater) from 13 chemically diverse field sites were assayed for their ability to consume acetylene (C2H2). Over incubation periods ranging ∼ 10 80 days, selected 7 of the tested displayed significant C2H2 removal. No formation ethylene was noted in these incubations; therefore, consumption could be attributed hydratase (AH) rather than nitrogenase activity. This putative AH (PAH) activity observed only 21% total samples, while amplification genes extracted...
We examined the potential for CH4 oxidation to be coupled with oxygen derived from dissimilatory reduction of perchlorate, chlorate, or via chlorite (ClO(-) 2) dismutation. Although ClO(-) 4 and 3 could inferred accumulation chloride ions either in spent media soil slurries prepared exposed freshwater lake sediment, neither these oxyanions evoked methane when added anaerobic mixed cultures enriched methanotrophs. In contrast, 2 amendment elicited such activity. Methane (0.2 kPa) was...
The Herman Pit, once a mercury mine, is an impoundment located in active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed mostly CO₂ with some CH₄ present. δ(13)CH₄ value suggested complex origin for the methane: i.e., thermogenic component plus biological methanogenic portion. relatively (12)C-enriched reworking ebullitive methane methanotrophic bacteria. Therefore, we tested bottom sediments their ability consume...
We report the first study of stable carbon isotope fractionation during microbial fermentation acetylene (C 2 H ) in sediments, sediment enrichments, and bacterial cultures.Kinetic effects (KIEs) averaged 3.7 -0.5& for slurries prepared with collected at an intertidal mudflat San Francisco Bay 2.7 -0.2& a pure culture Pelobacter sp.isolated from these sediments.A similar KIE 1.8 -0.7& was obtained methanogenic enrichments derived freshwater Searsville Lake, California.However, C uptake by...
A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93 T , was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. capable acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 in presence 10–45 g l −1 NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed that represented a member genus Syntrophotalea with highest sequence similarities to acetylenica DSM 3246 (96.6 %),...
Understanding the complex metabolisms of microbial communities in contaminated groundwaters is a challenge. PCE and TCE are among most common groundwater contaminants United States that, when exposed to certain minerals, exhibit unique abiotic degradation pathway which C 2 H product.
Acetylene fermentation is a rare metabolism that was serendipitously discovered during C2H2-block assays of N2O reductase. Here, we report the genome sequences two type strains acetylene-fermenting Pelobacter acetylenicus, freshwater bacterium DSM 3246 and estuarine 3247.
ABSTRACT Acetylene fermentation is a rare metabolism that was previously reported as being unique to Pelobacter acetylenicus . Here, we report the genome sequence of sp. strain SFB93, an acetylene-fermenting bacterium isolated from sediments collected in San Francisco Bay, CA.
The isolation of Bradyrhizobium strain I71 expands the distribution acetylene-consuming microbes to include a group economically important microorganisms. Members are well studied for their abilities improve plant health and increase crop yields by providing bioavailable nitrogen.
Use of methyl bromide (MeBr) as a quarantine, commodity, or structural fumigant is under scrutiny because its release to the atmosphere contributes depletion stratospheric ozone. A closed-system bioreactor consisting 0.5 L growing culture previously described bacterium, strain IMB-1, removed MeBr (>110 μmol L-1) from recirculating air. Strain IMB-1 grew slowly high cell densities in using sole carbon and energy source. Bacterial oxidation produced CO2 hydrobromic acid (HBr), which required...
Acetylene fermentation assays, nitrogen fixation and growth studies were performed with Pelobacter sp. strain SFB93 acetylenicus DSM3246. Data includes concentrations of acetylene ethylene over time, measured OD680 cell counts.