A5020211348- Research on Leishmaniasis Studies
- Emergency and Acute Care Studies
- Clinical Reasoning and Diagnostic Skills
- Sepsis Diagnosis and Treatment
- Extracellular vesicles in disease
- Protein Tyrosine Phosphatases
- Primary Care and Health Outcomes
- Trauma and Emergency Care Studies
- Parasites and Host Interactions
- Health Policy Implementation Science
- Trypanosoma species research and implications
- Health Sciences Research and Education
- Viral Infections and Vectors
- Pelvic and Acetabular Injuries
- Early Childhood Education and Development
- Telemedicine and Telehealth Implementation
- Eosinophilic Disorders and Syndromes
- Nanoplatforms for cancer theranostics
- Interprofessional Education and Collaboration
- Nursing Roles and Practices
- Macrophage Migration Inhibitory Factor
- Children's Physical and Motor Development
- Cardiac, Anesthesia and Surgical Outcomes
- Global Health Workforce Issues
- IL-33, ST2, and ILC Pathways
Provincial Health Services Authority
2019-2024
Medizinische Hochschule Hannover
2015-2020
University of British Columbia
2018-2020
Vancouver Coastal Health
2019
Royal Columbian Hospital
2017-2018
Fraser Health
2017-2018
Western University
2018
Université Laval
2018
University of Ottawa
2018
McGill University Health Centre
2008-2016
Despite several studies describing the secretion of exosomes by Leishmania in vitro, observation their formation and release vivo has remained a major challenge. Herein, we show that constitutively secretes within lumen sand fly midgut through mechanism homologous to mammalian pathway. Through egestion experiments, demonstrate are part inoculum co-egested with parasite during insect's bite, possibly influencing host infectious process. Indeed, co-inoculation mice footpads L. plus...
Released by many eukaryotic cells, the exosomes are 40–100 nm vesicles shown to operate over complex processes of cell-cell communication. Among metazoan cell lineages known generate is mononuclear phagocyte lineage, a lineage that parasites such as Leishmania subvert host cells. We previously reported mouse macrophage signaling and functions modified once co-incubated with exoproteome promastigotes. Using mass spectrometry analysis, we were curious further compare content purified released...
Protozoan parasites of Leishmania genus are able to successfully infect their host macrophage due multiple virulence strategies that result in its deactivation. Recent studies suggest GP63 be a critical factor modulation many molecules, including protein tyrosine phosphatases (PTPs) and transcription factors (TFs). Additionally, we others recently reported Leishmania-released exosomes can participate pathogenesis. Exosomes 40–100 nm vesicles freed by eukaryotic cells. To better understand...
Protozoan parasites of genus Leishmania are the causative agents leishmaniasis. These digenetic microorganisms undergo a marked environmental temperature shift (TS) during transmission from sandfly vector (ambient temperature, 25-26°C) to mammalian host (37°C). We have observed that this TS induces rapid and dramatic increase in protein release mexicana (cutaneous leishmaniasis) within 4 h. Proteomic identification TS-induced secreted proteins revealed 72 proteins, majority which lack signal...
Leishmania parasites are able to secure their survival and propagation within host by altering signalling pathways involved in the ability of macrophages kill pathogens or engage adaptive immune system. An important step this evasion process is activation protein tyrosine phosphatase SHP-1 Leishmania. has been shown directly inactivate JAK2 Erk1/2 play a role negative regulation several transcription factors macrophage activation. These alterations contribute inactivation critical functions...
Priming of the mucosal immune system during postnatal period substantially influences host-microbial interaction and susceptibility to immune-mediated diseases in adult life. The underlying mechanisms are ill defined. Here we show that shortly after birth, CD4 T cells populate preformed lymphoid structures small intestine quickly acquire a distinct transcriptional profile. T-cell recruitment is independent microbial colonization innate or adaptive stimulation but requires β7 integrin...
Leishmania alternates between two morphologically different stages, promastigotes and amastigotes. While the majority of reports focused on how promastigote form can alter macrophage (Mphi) signaling function, fewer investigated alterations mediated by amastigotes, there is a lack comparative studies. In this study, we performed comparison ability both forms parasite to Mphi functions. Here, show that amastigotes were able rapidly activate host protein tyrosine phosphatases (PTPs),...
Childcare is a critical target for promoting children’s physical activity (PA) and literacy (PL). With emerging evidence about the efficacy of policy capacity-building strategies, more information how to bring these strategies scale needed. This paper describes implementation at Appetite Play (ATP), intervention childcare providers, examines impact on early years providers’ capacity address PA. The ATP evaluation was natural experiment that utilized mixed methods concurrent parallel design...
The protozoan parasite Giardia lamblia enhances bile secretion and microbiota–mediated acid deconjugation in mice, leading to growth impairment.
Nitric oxide (NO) is a potent molecule involved in the cytotoxic effects mediated by macrophages (MØ) against microorganisms. We previously reported that Src homology 2 domain phosphotyrosine phosphatase 1 (SHP-1)-deficient cells generate greater amount of NO than wild-type response to interferon-gamma (IFN-gamma). also Leishmania-induced MØ SHP-1 activity needed for survival parasite within phagocytes through attenuation NO-dependent and NO-independent mechanisms. In present study, we...
SUMMARY Leishmania protozoa are obligate intracellular parasites that reside in the phagolysosome of host macrophages and cause a large spectrum pathologies to humans known as leishmaniases. The outcome disease is highly dependent on parasite species its ascribed virulence factors immune status host. Characterization genome composition non-pathogenic could ultimately open new horizons developmental biology also monitoring. Here, we provide evidence lizard tarentolae expresses an Amastin...
<h3>Background:</h3> In 2013, the Canadian Institutes of Health Research funded 12 community-based primary health care research teams to develop evidence-based innovations. We aimed explore scalability these <h3>Methods:</h3> this cross-sectional study, we invited rate their innovations for scalability. Based on a systematic review, developed self-administered questionnaire with 16 assessment criteria grouped into 5 dimensions (theory, impact, coverage, setting and cost). Teams completed...
The rapid identification of deterioration in the pediatric population is complex, particularly emergency department (ED). A comprehensive multi-faceted Pediatric Early Warning System (PEWS) might maximize early recognition clinical and provide a structured process for reassessment escalation care. objective study was to evaluate implementation fidelity, effectiveness, utility 5-component PEWS implemented ED an urban public general hospital British Columbia, Canada, guide provincial scale...
Abstract Objective: The purpose of this study was to examine the dissemination healthy eating component Appetite Play at scale using Reach, Effectiveness, Adoption, Implementation, Maintenance (RE-AIM) framework. Design: capacity-building intervention is a set evidence-informed implementation strategies aimed enhancing adoption recommended practices for promoting and active play in early years settings. evaluation pragmatic, employing both quantitative (surveys) qualitative (interviews) data...
Abstract The objectives of this paper are to: (1) identify contextual factors such as policy that impacted the implementation community-based primary health care (CBPHC) innovations among 12 Canadian research teams and (2) describe strategies used by to address influencing CBPHC innovations. In settings, consideration when implementing change has been recognized critically important success. However, rarely recorded, analyzed or considered change. lack negative implications not only for...
Abstract Aim: To describe activities and outcomes of a cross-team capacity building strategy that took place over five-year funding period within the broader context 12 community-based primary health care (CBPHC) teams. Background: In 2013, Canadian Institutes Health Research funded CBPHC Teams (12-Teams) to conduct innovative cross-jurisdictional research improve delivery high-quality Canadians. This signature initiative also aimed enhance among an interdisciplinary group trainees,...
AimTo describe the process by which 12 community-based primary health care (CBPHC) research teams worked together and fostered cross-jurisdictional collaboration, including collection of common indicators with goal using same measures data sources. BACKGROUND: A pan-Canadian mechanism for measurement impact innovations across Canada is lacking. The Canadian Institutes Health Research its partners funded to conduct collaborate on development a set commonly collected indicators. METHODS:...
Introduction: Adoption of virtual health (VH) solutions in healthcare has been challenging; this changed rapidly after implementation physical distancing measures due to the COVID-19 pandemic. In response pandemic, British Columbia's Children's and Women's sub-specialty hospitals trained scaled up support equip staff clinicians use VH. Methods: Ninety-minute live online training workshops frequently updated materials were offered for 6 weeks. Training was monitored via feedback collected at...
Abstract Background: The rapid identification of deterioration in the pediatric population is complex, particularly emergency department (ED). A comprehensive multi-faceted Pediatric Early Warning System (PEWS) might maximize early recognition clinical and provide a structured process for reassessment escalation care. objective study was to evaluate implementation fidelity, effectiveness, utility 5-component PEWS implemented ED an urban public general hospital British Columbia, Canada, guide...
The recovery process from traumatic injuries, and the potential for complications, extends beyond time of hospital discharge. In 2014, Fraser Health Trauma Network established outpatient clinics to provide follow-up care trauma patients after discharge hospital. following research questions were asked: Which services commonly performed by our how satisfied with they received at clinics? A survey was distributed their clinic visit assess overall satisfaction areas improvement. retrospective...