Three species of cichlids belonging to the genus Symphysodon have demonstrated interspecific and intraspecific variation in nucleolus organizer regions (NOR) detected with silver nitrate. In order understand evolution this marker genus, structural variability these sequences mitotic chromosomes from aequifasciatus, discus haraldi was investigated using both nitrate impregnation hybridization 18S rRNA gene probe. For three species, two markers were intraspecifically interspecifically variable...

As part of a genetic screening program for wild Discus fishes, we analyzed karyotypes and cytogenetic characteristics <i>Symphysodon aequifasciatus</i>, <i>S. discus</i> haraldi</i> using C-banding fluorescent in situ hybridization (FISH) with the <i>Rex</i>3 retrotransposon 5S rDNA probes mitotic meiotic chromosomes. In 3 species, diploid chromosome number was 2n = 60 contained predominantly meta-submetacentric showed blocks constitutive...

Four populations of Astyanax aff. fasciatus the upper rio Tibagi (municipal district Ponta Grossa, Paraná State, Brazil), had their karyotypes and morphometry analyzed. The cytogenetic data show occurrence distinct (cytotypes), here named cytotype A, with 2n=48 chromosomes (6m+18sm+14st+10a), B, 2n=50 (8m+18sm+14st+10a) C, (8m+18sm+14st+10a). distribution pattern constitutive heterochromatin was very similar between cytotypes A but diverged in relation to C. Distinct may occur sympatry...

Transposable elements (TEs) have the potential to produce broad changes in genomes of their hosts, acting as a type evolutionary toolbox and generating collection new regulatory coding sequences. Several TE classes been studied Neotropical cichlids; however, information gained from these studies is restricted physical chromosome mapping, whereas genetic diversity TEs remains unknown. Therefore, genomic organization non-LTR retrotransposons Rex1, Rex3, Rex6 five Amazonian cichlid species was...

This study assessed the viability of combined use coal processing waste (CPW), rice (RPW), poultry agro-industry sludge (PAS), synthetic gypsum (SG), topsoil (TS), and B horizon soil (BH) to reclaim areas degraded by mining. A Classification, Potentiality, Quantity/viability, Application (CPQvA) systematic was employed assess feasibility using these materials construct technosols. An experiment consisting five technosols (T-1–T-5) replicated four times in a completely randomized block...

Distribution of 6 microsatellites and 5 transposable elements on the chromosomes <i>Semaprochilodus taeniurus </i>and <i>S. insignis</i>, commonly referred to as Jaraqui, was performed using their physical mapping with fluorescence in situ hybridization. In this study, we aim understand evolutionary dynamics genomes <i>S</i>. <i>taeniurus</i> insignis</i> by comparing position, abundance contribution repetitive sequences origins...

O carvão mineral brasileiro contém altos teores de impurezas (pirita e minerais rochas sedimentares), sendo necessário seu beneficiamento. Os rejeitos do beneficiamento demandam uma grande área física para a sua disposição, modificam topografia geram drenagem ácida minas (DAM). objetivo desse trabalho foi caracterizar um típico depósito na região carbonífera Santa Catarina, buscando separação três frações uso futuro: (a) fração geração energia; (b) enriquecida em silicatos; (c) com alto teor...

The genetic variability of the brown pencilfish Nannostomus eques was studied, based on an analysis sequences from control region (1084 bp) mitochondrial (mt)DNA in 125 individuals collected eight tributaries along upper (Açaituba, Miuá, Jaradi and Arixanã), middle (Demini), lower (Jacundá, Maguari Catalão) Rio Negro (Brazil). Phylogenetic inferences using mtDNA data N. revealed two evolutionary units. Genetic distance between them ranged 5·5 to 8·3% differed by 8·5–11·8% sister species...

The structure and organization of repetitive elements in fish genomes are still relatively poorly understood, although most these believed to be located heterochromatic regions. Repetitive considered essential evolutionary processes as hotspots for mutations chromosomal rearrangements, among other functions - thus providing new genomic alternatives regulatory sites gene expression. present study sought characterize DNA sequences the Semaprochilodus insignis (Jardine & Schomburgk, 1841)...

Hypsiboas species have been divided into seven groups using morphological and genetic characters, but for most of the species, there is no cytogenetic information available. A analysis conventional staining, C-banding, silver fluorescence in situ hybridization (FISH) with telomeric sequence probes were used to investigate karyotype Amazon genus belonging following intrageneric groups: H. punctatus (H. cinerascens), semilineatus boans, geographicus, wavrini), albopunctatus lanciformis,...

Oecomys Thomas, 1906 is one of the most diverse and widely distributed genera within tribe Oryzomyini. At least sixteen species in this genus have been described to date, but it believed contains undescribed species. Morphological, molecular cytogenetic study has revealed an uncertain taxonomic status for several species, suggesting presence a complex The present work had goal contributing genetic characterization Brazilian Amazon. Thirty specimens were collected from four locations Amazon...

The Amazon aquatic ecosystems have been modified by the human population growth, going through changes in their water bodies and biota. spectacled alligator (Caiman crocodilus crocodilus) has a wide distribution adaptability to several environments, even those polluted ones. This study aimed investigate if Caiman species living urban streams of Manaus city (Amazonas State, Brazil) is affected environmental pollution. For that, it was used classical molecular cytogenetic procedures, addition...

The Amazon has abundant rivers, streams, and floodplains in both polluted nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences mitotic chromosomes erythrocyte micronuclei tamoatás from environments assess possible genotoxic effects these environments. Individuals were collected Manaus, Amazonas (AM), submitted classical molecular cytogenetic techniques, as well a blood micronucleus test. Diploid number equal 60 are present...

Mitochondrial DNA ( mtDNA) sequences of the marbled hatchetfish Carnegiella strigata , an ornamental fish exported from Negro River, was examined to determine its genetic diversity and population structure in blackwater rivers (Negro Uatumã Rivers) central Amazon. Analyses a 646 bp fragment ATPase 6/8 mtDNA gene revealed two monophyletic lineages C. with considerable distance between them (10–12%), suggesting that these should not be considered single stock. Furthermore, there were strong...

Lizards of the family Teiidae (infraorder Scincomorpha) were formerly known as Macroteiidae. There are 13 species such lizards in Amazon, genera Ameiva (Meyer, 1795), Cnemidophorus (Wagler, 1830), Crocodilurus (Spix, 1825), Dracaena (Daudin, 1801), Kentropyx 1825) and Tupinambis 1802). Cytogenetic studies this group restricted to karyotype macrostructure. Here we give a compilation cytogenetic data Teiidae, including classic molecular analysis ameiva (Linnaeus, 1758), sp.1, calcarata...

Transposable elements have driven genome evolution and plasticity in many ways across a range of organisms. Different types biotic abiotic stresses can stimulate the expression or transposition these mobile elements. Here, we cytogenetically analyzed natural fish populations same species living under different environmental conditions to test influence organization transposable their genome. Differential behavior was observed for markers Rex 1, 3, 6 chromosomes individuals but coming from...

Fish of the family Cichlidae are recognized as an excellent model for evolutionary studies because their morphological and behavioral adaptations to a wide diversity explored ecological niches. In addition, has dynamic genome with variable structure, composition karyotype organization. Microsatellites represent most genomic component better understanding organization may help clarify role repetitive DNA elements in mechanisms chromosomal evolution. Thus, this study, microsatellite sequences...