A5077854507- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Cancer Research and Treatments
- Nutrition, Genetics, and Disease
- Genetic Mapping and Diversity in Plants and Animals
- Diet and metabolism studies
- Stress Responses and Cortisol
- CRISPR and Genetic Engineering
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Liver physiology and pathology
- Animal Virus Infections Studies
- Epigenetics and DNA Methylation
- Genetic and phenotypic traits in livestock
- Animal Behavior and Welfare Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- CAR-T cell therapy research
- Gut microbiota and health
- Reproductive Physiology in Livestock
- Digital Holography and Microscopy
- Neuroinflammation and Neurodegeneration Mechanisms
- Ultrasound and Hyperthermia Applications
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
University of Illinois Urbana-Champaign
2015-2024
University of Illinois System
2013-2023
Urbana University
1996-2017
University of Georgia
1988-1992
Dairy Management
1988
Abstract Background The domestic pig (Sus scrofa) is important both as a food source and biomedical model given its similarity in size, anatomy, physiology, metabolism, pathology, pharmacology to humans. draft reference genome (Sscrofa10.2) of purebred Duroc female established using older clone-based sequencing methods was incomplete, unresolved redundancies, short-range order orientation errors, associated misassembled genes limited utility. Results We present 2 annotated highly contiguous...
Microglia play a vital role maintaining brain homeostasis but can also cause persistent neuroinflammation. Short-chain fatty acids (SCFAs) produced by the intestinal microbiota have been suggested to regulate microglia inflammation indirectly signaling through gut-brain axis or directly reaching brain. The present work evaluated anti-inflammatory effects of SCFAs on lipopolysaccharide (LPS)-stimulated from mice fed inulin, soluble fiber that is fermented produce in vivo, and applied primary...
The large size of the pig and its similarity in anatomy, physiology, metabolism, genetics to humans make it an ideal platform develop a genetically defined, animal model cancer. To this end, we created transgenic "oncopig" line encoding Cre recombinase inducible porcine transgenes KRASG12D TP53R167H, which represent commonly mutated oncogene tumor suppressor human cancers, respectively. Treatment cells derived from these oncopigs with adenovirus (AdCre) led TP53R167H expression, rendered...
To gain insight into host-microbe interactions in a piglet model, functional genomics approach was used to address the working hypothesis that transcriptionally regulated genes associated with promoting epithelial barrier function are activated as defensive response intestinal microbiota. Cesarean-derived germfree (GF) newborn piglets were colonized adult swine feces, and villus crypt cell transcriptomes from GF neonatal compared using laser-capture microdissection high-density porcine...
Pigs (Sus scrofa) provide relevant biomedical models to dissect complex diseases due their anatomical, genetic, and physiological similarities with humans. Aberrant DNA methylation has been linked many of these is associated gene expression; however, the functional differences between porcine human patterns are largely unknown. RNA was isolated from eight tissue samples (fat, heart, kidney, liver, lung, lymph node, muscle, spleen) adult female Duroc utilized for pig genome sequencing...
Repurposing of existing cancer drugs to overcome their physical limitations, such as insolubility, represents an attractive strategy achieve enhanced therapeutic efficacy and broaden the range clinical applications. Such approach also promises offer substantial cost savings in drug development efforts. Here we repurposed FDA-approved topical agent bexarotene (Targretin), currently limited use for cutaneous manifestations T-cell lymphomas, re-engineer it solid tumor applications by forming...
// Kyle M. Schachtschneider 1, 2, 3 , Regina Schwind Kwame A. Darfour-Oduro 1 Arun K. De Lauretta Rund Kuldeep Singh 4 Daniel R. Principe 5 Grace Guzman 6 Charles E. Ray Jr. 3, 7 Howard Ozer Ron C. Gaba and Lawrence B. Schook Department of Animal Sciences, University Illinois, Urbana, IL, USA 2 Breeding Genomics Centre, Wageningen University, Wageningen, The Netherlands Radiology, Illinois at Chicago, Veterinary Diagnostic Laboratory, College Medicine, Pathology, Division...
Iron deficiency is a common childhood micronutrient that results in altered hippocampal function and cognitive disorders. However, little known about the mechanisms through which neonatal iron long lasting alterations gene expression function. DNA methylation an epigenetic mark involved regulation by environmental factors. In this study, were assessed via reduced representation bisulfite sequencing RNA-seq on samples from previous study reporting hippocampal-based learning memory porcine...
Abstract The domestic pig ( Sus scrofa ) is important both as a food source and biomedical model with high anatomical immunological similarity to humans. draft reference genome (Sscrofa10.2) of purebred Duroc female established using older clone-based sequencing methods was incomplete unresolved redundancies, short range order orientation errors associated misassembled genes limited its utility. We present two annotated highly contiguous chromosome-level assemblies created more recent long...
Due to the increasing human life expectancy and limited supply of healthcare resources, strategies promote healthy aging reduce associated functional deficits are public health importance. The gut microbiota, which remodels with age, has been identified as a significant contributor process that is modifiable by diet. Since prebiotic dietary components such inulin have shown impart positive benefits regards aging, this study used C57Bl6 mice investigate whether 8 weeks on 2.5 % enhanced...
Gut inflammation can trigger neuroinflammation and is linked to mood disorders. Microbiota-derived short-chain fatty acids (SCFAs) modulate microglia, yet the mechanism remains elusive. Since microglia do not express free-fatty acid receptor (FFAR)2, but intestinal epithelial cells (IEC) peripheral myeloid do, we hypothesized that SCFA-mediated FFAR2 activation within gut or leukocytes may impact inflammation. To test this hypothesis, developed a tamoxifen-inducible conditional knockout...
T cells are activated by binding of the cell receptor (TCR) to a peptide-major histocompatibility complex (MHC) (pMHC) expressed on surface antigen presenting cells. Various models have predicted that activation is limited narrow window affinities (or dissociation rates) for TCR–pMHC interaction and above or below this window, will fail undergo activation. However, date there not been TCRs with sufficiently high in order test hypothesis. In report we examined activity CD8-negative line...
Activation of microglia, the resident immune cells central nervous system, has been related to etiology and progression neurodegenerative diseases; thus, finding novel approaches suppress neuroinflammatory process is utmost relevance.The anti-inflammatory activity whey Cu-, Fe-, Zn-binding peptides their possible underlying mechanism action were evaluated in microglia. Whey metal-binding decreased nitric oxide production tumor necrosis factor α (TNF-α) at mRNA protein levels by stimulated...
The prolonged and sex-dependent impact of maternal immune activation (MIA) during gestation on the molecular pathways amygdala, a brain region that influences social, emotional, other behaviors, is only partially understood. To address this gap, we investigated effects viral-elicited MIA amygdala transcriptome pigs, species high developmental homology to humans. Gene expression levels were measured using RNA-Seq for 3-week old female male offspring from control groups. Among 403 genes...
Insulin-like growth factor-I (IGF-I) is a mitogenic polypeptide postulated to mediate the effect of GH on adipose tissue development. To determine if could be mediated by local production IGF-I, we have characterized IGF-I RNA expression, secretion, and binding protein activity in primary preadipocyte cultures derived from porcine tissues. acutely regulated abundance multiple transcripts resulted 2- fold increase secreted immunoreactive (iIGF-I) medium conditioned for 48 h relative those not...
Abstract Although survival has improved in recent years, the prognosis of patients with advanced pancreatic ductal adenocarcinoma (PDAC) remains poor. Despite substantial differences anatomy, physiology, genetics, and metabolism, overwhelming majority preclinical testing relies on transgenic mice. Hence, while mice have allowed for tremendous advances cancer biology, they been a poor predictor drug performance/toxicity clinic. Given greater similarity sus scrofa pigs to humans, we engineered...
// Ron C. Gaba 1 , 2 Lobna Elkhadragy F. Edward Boas 3 Sulalita Chaki 4 Hanna H. Chen Mohammed El-Kebir 5 Kelly D. Garcia 6 Eileena Giurini Grace Guzman Francesca V. LoBianco 7 Mario Neto Jordan L. Newson Aisha Qazi Maureen Regan 8 Lauretta A. Rund Regina M. Schwind Matthew Stewart 9 Faith Thomas Herbert E. Whiteley Jiaqi Wu Lawrence B. Schook 10 and Kyle Schachtschneider Department of Radiology, University Illinois at Chicago, IL, USA Pathology, Memorial Sloan Kettering Cancer Center, New...
With increasing age, microglia shift toward a pro-inflammatory phenotype that may predispose individuals to neurodegenerative disease. Because fiber fermentation in the colon produces bioactive short-chain fatty acids (SCFAs; e.g., acetate, butyrate, and propionate) signal through gut-brain axis, dietary prevent or reverse age-related dysregulation of microglia. Adult (3-4 months old) aged (23-24 male female mice were given ad libitum access modified AIN-93M diet with 1% cellulose same 2.5...
Abstract Three sources of help for the development a CD8+ CTL response have been described: CD4+ direct and indirect pathways pathway. In an effort to understand minimal requirements in vivo, we bred mice transgenic 2C TCR onto RAG(-/-) background. The T cells this animal are exclusively CTLs single specificity, they exhibit altered thymic maturation compared with that from TCR/RAG(+/+) mice. TCR/RAG(-/-) can be activated high level vivo by administration self-MHC-restricted antigenic...
The anatomical locations of proopiomelanocortin (POMC) and luteinizing hormone-releasing hormone (LHRH) neurons were examined in brain tissue from peripubertal female calves mature, luteal-phase cows. Biotin-avidin-peroxidase procedures used for single- double-labeled immunostaining. LHRH perikarya formed a loosely arranged continuum, extending posteriorly the diagonal band Broca, passing through medial lateral preoptic areas, ending within anterior hypothalamic area. fibers, apparently...