A5101952999- Tendon Structure and Treatment
- Osteoarthritis Treatment and Mechanisms
- Veterinary Equine Medical Research
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Orthopedic Infections and Treatments
- Orthopedic Surgery and Rehabilitation
- Shoulder Injury and Treatment
- American Constitutional Law and Politics
- Sports injuries and prevention
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Orthopaedic implants and arthroplasty
- Religion and Society Interactions
- Bee Products Chemical Analysis
- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Connective tissue disorders research
- Bone fractures and treatments
- Exercise and Physiological Responses
- Academic Freedom and Politics
- Cancer-related molecular mechanisms research
Cleveland Clinic
2025
Johns Hopkins University
2023-2025
University of Illinois Urbana-Champaign
2014-2023
University of Illinois System
2016-2020
Center for Genomic Science
2007-2015
Urbana University
2015
The University of Texas at Dallas
2014
University of Veterinary Medicine
2014
University of Wisconsin–Madison
2010
Johns Hopkins Hospital
2010
The long-term integrity of an articulating joint is dependent upon the nourishment its cartilage component and protection surface from friction-induced wear. Loss-of-function mutations in lubricin (a secreted glycoprotein encoded by gene PRG4) cause human autosomal recessive disorder camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). A major feature CACP precocious failure. In order to delineate mechanism which protects joints, we studied expression Prg4 mRNA during mouse...
The long-term integrity of an articulating joint is dependent upon the nourishment its cartilage component and protection surface from friction-induced wear. Loss-of-function mutations in lubricin (a secreted glycoprotein encoded by gene PRG4) cause human autosomal recessive disorder camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). A major feature CACP precocious failure. In order to delineate mechanism which protects joints, we studied expression Prg4 mRNA during mouse...
Abstract Numerous in vitro culture models have been developed for the investigation of chondrocyte and cartilage biology. In this study, we investigated stability chondrocytic phenotype monolayer, aggregate, pellet, explant assessed effects recombinant human bone morphogenetic protein 2 (rhBMP-2) serum supplementation on each model. Phenotypic were by analyses procollagen type II, aggrecan, (V+C)− fibronectin, I messenger RNA expression. monolayer cultures, noted a characteristic loss II...
Abstract Objective —To determine whether fibroblast growth factor-2 (FGF-2) treatment of equine mesenchymal stem cells (MSCs) during monolayer expansion enhances subsequent chondrogenesis in a 3-dimensional culture system. Animals —6 healthy horses, 6 months to 5 years age. Procedures —Bone marrow–derived MSCs were obtained from horses. First-passage seeded as monolayers at 10,000 cells/cm 2 and medium containing 0, 1, 10, or 100 ng FGF-2/mL. After days, transferred pellet cultures (200,000...
Abstract Objective —To compare viability and biosynthetic capacities of cells isolated from equine tendon, muscle, bone marrow grown on autogenous tendon matrix. Sample Population —Cells 4 young adult horses. Procedures were isolated, expanded, cultured cell-free matrix for 7 days. Samples analyzed cell viability, proteoglycan synthesis, collagen mRNA expression type I, III, cartilage oligomeric protein (COMP). Results —Tendon- muscle-derived required less time to reach confluence (approx 2...
Tendinitis is a common and performance-limiting injury in athletes. This study describes the value of intralesional tendon-derived progenitor cell (TDPC) injections equine flexor tendinitis. Collagenase-induced tendinitis was created both front superficial digital (SDF) tendons. Four weeks later, forelimb tendon lesions were treated with 1 × 107 autogenous TDPCs or saline. also induced by collagenase one hind SDF tendon, to survival distribution DiI-labeled 1, 2, 4, 6 after injection. The...
Abstract Aseptic loosening is the most common cause of orthopaedic implant failure. This process thought to be due osteolysis induced by implant‐derived wear particles. Teitelbaum and colleagues have recently developed a promising murine calvarial model particle‐induced osteolysis. However, prior this study, had only been assessed qualitatively. We now report reproducible, quantitative version osteolysis, in which extent (and repair) entire parietal bones histomorphometry contact...
A growing body of evidence suggests that systemic hormones and peptide growth factors may exert their effects on cell differentiation in part through regulation the division cycle. We hypothesized thyroid hormone regulates terminal plate chondrocytes controlling cycle progression at G1/S restriction point. Our results support this hypothesis by demonstrating treatment epiphyseal with under chemically defined conditions arrest DNA synthesis onset differentiation, indicating is one factor...
Abstract Bacterial endotoxin may contribute to aseptic loosening of orthopedic implants even in the absence clinical or microbiological evidence infection. One potential source during is systemically circulating endotoxin, derived from intestinal flora, minor infections, dental procedures, that bind wear particles. The current study demonstrates accumulates when ‘endotoxin‐free’ titanium and polyethylene particles are implanted on murine calvaria. Time‐course experiments using germ‐free mice...
Journal Article Simulating the Effects of Alternative Vote in 2010 UK General Election Get access David Sanders, Sanders University Essexsanders@essex.ac.uk whiteley@essex.ac.uk Search for other works by this author on: Oxford Academic Google Scholar Harold D. Clarke, Clarke Essex and Texas at Dallasclarke475@msn.com Marianne C. Stewart, Stewart Dallasmstewart@utdallas.edu Paul Whiteley Parliamentary Affairs, Volume 64, Issue 1, January 2011, Pages 5–23, https://doi.org/10.1093/pa/gsq042...
To compare in vitro expansion, explant colonization, and matrix synthesis of equine tendon- bone marrow-derived cells response to insulin-like growth factor-I (IGF-I) supplementation.Cells isolated from 7 young adult horses.Tendon- progenitor were isolated, evaluated for yield, cultured on autogenous cell-free tendon days. Samples analyzed cell viability expression collagen type I, III, cartilage oligomeric protein mRNAs. Collagen glycosaminoglycan syntheses quantified over a 24-hour...
// Ron C. Gaba 1 , 2 Lobna Elkhadragy F. Edward Boas 3 Sulalita Chaki 4 Hanna H. Chen Mohammed El-Kebir 5 Kelly D. Garcia 6 Eileena Giurini Grace Guzman Francesca V. LoBianco 7 Mario Neto Jordan L. Newson Aisha Qazi Maureen Regan 8 Lauretta A. Rund Regina M. Schwind Matthew Stewart 9 Faith Thomas Herbert E. Whiteley Jiaqi Wu Lawrence B. Schook 10 and Kyle Schachtschneider Department of Radiology, University Illinois at Chicago, IL, USA Pathology, Memorial Sloan Kettering Cancer Center, New...
This study determined the effects of a plasma-sprayed hydroxyapatite/tricalcium phosphate (HA/TCP) coating on osseointegration titanium alloy implants in lapine, distal femoral intramedullary model. The HA/TCP were assessed at 1, 3, and 6 months after implant placement. significantly increased new bone apposition onto surfaces all time points. ceramic also stimulated formation middle levels implants. Fluorescent labeling indicated that occurred primarily during first 3 implantation, with...
Abstract Objective —To determine whether expansion of equine mesenchymal stem cells (MSCs) by use fibroblast growth factor-2 (FGF-2) prior to supplementation with dexamethasone during the chondrogenic pellet culture phase would increase chondrocytic matrix markers without stimulating a hypertrophic phenotype. Sample Population —MSCs obtained from 5 young horses. Procedures —First-passage monolayer MSCs were supplemented medium containing FGF-2 (0 or 100 ng/mL). Confluent transferred cultures...
Abstract Implant success is dependent in part on the interaction of implant with surrounding tissues. Porous tantalum implants (Trabecular Metal™, TM) have been shown to excellent osseointegration. Machining this material complex shapes close tolerances difficult because its open structure and ductile nature metallic tantalum. Conventional machining results occlusion most surface porosity by smearing soft metal. This study compared TM samples finished three processing techniques:...