A5055516290- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- MicroRNA in disease regulation
- Genetic Neurodegenerative Diseases
- Histone Deacetylase Inhibitors Research
- Nanoplatforms for cancer theranostics
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Mesenchymal stem cell research
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Neuroscience and Neural Engineering
- Cardiac Fibrosis and Remodeling
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- ATP Synthase and ATPases Research
- Electron Spin Resonance Studies
- RNA Interference and Gene Delivery
- Peripheral Artery Disease Management
- bioluminescence and chemiluminescence research
Adam Mickiewicz University in Poznań
2019-2025
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2021
Erasmus MC
2020
Jagiellonian University
2015-2019
Codarts Rotterdam
2019
Erasmus MC Cancer Institute
2014
Hot-spot mutations in the promoter region of telomerase reverse transcriptase (TERT mutations) occur frequently cutaneous and conjunctival melanoma are exceedingly rare uveal melanoma. No information is available on presence these melanocytic precursor lesion primary acquired melanosis (PAM). We tested a cohort melanomas as well benign premalignant lesions for TERT order to elucidate role tumor progression.TERT mutation analysis fresh DNA from formalin-fixed, paraffin-embedded specimens was...
Abstract Rhabdomyosarcoma (RMS) is an aggressive soft tissue cancer characterized by disturbed myogenic differentiation. Here we report a role for the oxidative stress response factor HO-1 in progression of RMS. We found that was elevated and its effector target miR-206 decreased RMS cell lines clinical primary tumors more alveolar phenotype (aRMS). In embryonal (eRMS), expression induced Pax3/7-FoxO1, aRMS hallmark oncogene, followed drop levels. Inhibition tin protoporphyrin (SnPP) or...
Cardiovascular diseases (CVDs) include atherosclerosis, which is an inflammatory disease of large and medium vessels that leads to atherosclerotic plaque formation. The key factors contributing the onset progression atherosclerosis pro-inflammatory cytokines interferon (IFN)α IFNγ pattern recognition receptor (PRR) Toll-like 4 (TLR4). Together, they trigger activation IFN regulatory (IRFs) signal transducer activator transcription (STAT)s. Based on their promoting role in we hypothesized...
Huntington’s disease (HD) is a polyglutamine neurodegenerative involving pathogenesis within the striatum and cerebral cortex neurodevelopmental component, particularly in juvenile HD form (JOHD). We established fused dorsal-ventral system, imitating interaction single organoid to discover impairments at level of cell populations. found range early pathogenic phenotypes indicating that brain development affected by impaired neurogenesis. The occurred already early-stage 60-day organoids...
Huntington’s disease (HD) is a polyglutamine neurodegenerative involving pathogenesis within the striatum and cerebral cortex neurodevelopmental component, particularly in juvenile HD form (JOHD). We established fused dorsal-ventral system, imitating interaction single organoid to discover impairments at level of cell populations. found range early pathogenic phenotypes indicating that brain development affected by impaired neurogenesis. The occurred already early-stage 60-day organoids...
MicroRNA-378a, highly expressed in skeletal muscles, was demonstrated to affect myoblasts differentiation and promote tumour angiogenesis. We hypothesized that miR-378a could play a pro-angiogenic role muscle may be involved regeneration after ischaemic injury mice.Silencing of murine C2C12 did not but impaired their secretory angiogenic potential towards endothelial cells. knockout (miR-378a-/-) mice resulted decreased number CD31-positive blood vessels arterioles gastrocnemius muscle. In...
Abstract The expression of the matricellular protein periostin has been associated with glioma progression. In previous work we found an association angiogenesis. Here, screen gliomas for POSTN and identify cells that express in human gliomas. addition, study role vitro model was investigated by RT-PCR immunohistochemistry. used double labeling situ RNA techniques to expressing cells. To investigate function periostin, silenced a 3D angiogenesis model. Periostin elevated pilocytic...
Magnetic nanoparticles are extensively studied for their use in diagnostics and medical therapy. The behavior of after adding them to cell culture is an essential factor (i.e., whether they attach a membrane or penetrate the enter into cell). present studies aimed demonstrate application electron spin resonance (ESR) as suitable technique monitoring entering cells during endocytosis process. model were composed magnetite iron (II, III) oxide core functionalized with organic unit containing...
Huntington's disease (HD) is a polyglutamine neurodegenerative involving pathogenesis within the striatum and cerebral cortex neurodevelopmental component, particularly in juvenile HD form (JOHD). We established fused dorsal-ventral system, imitating interaction single organoid to discover impairments at level of cell populations. found range early pathogenic phenotypes indicating that brain development affected by impaired neurogenesis. The occurred already early-stage 60-day organoids...
Abstract BACKGROUND The expression of the matricellular protein periostin has recently been associated with glioma progression and angiogenesis. aim present study was to identify cellular source in human gliomas role an vitro model for MATERIAL AND METHODS various types grades brain tumors investigated at genetics level by RT-PCR immunohistochemistry. In addition, samples studied double labeling situ RNA techniques. Subsequently, silencing a 3D angiogenesis model, its effects on were...
<div>Abstract<p>Rhabdomyosarcoma (RMS) is an aggressive soft tissue cancer characterized by disturbed myogenic differentiation. Here we report a role for the oxidative stress response factor HO-1 in progression of RMS. We found that was elevated and its effector target miR-206 decreased RMS cell lines clinical primary tumors more alveolar phenotype (aRMS). In embryonal (eRMS), expression induced Pax3/7-FoxO1, aRMS hallmark oncogene, followed drop levels. Inhibition tin...
<div>Abstract<p>Rhabdomyosarcoma (RMS) is an aggressive soft tissue cancer characterized by disturbed myogenic differentiation. Here we report a role for the oxidative stress response factor HO-1 in progression of RMS. We found that was elevated and its effector target miR-206 decreased RMS cell lines clinical primary tumors more alveolar phenotype (aRMS). In embryonal (eRMS), expression induced Pax3/7-FoxO1, aRMS hallmark oncogene, followed drop levels. Inhibition tin...
<p>Scheme showing the proposed relation between Pax3-FoxO1 induced expression of HO-1 and miR-206 dependent pathway.</p>
<p>Comparison of SMS-CTR eRMS (SMS) and CW9019 aRMS (CW) cell lines cultured under standard conditions. Expression VEGF, VEGFR-1, VEGFR-2, HIF-1, HIF-2 miR-146a genes.</p>
<p>Detailed description of methods used in the study with Supplementary Figures legends</p>
<p>Expression of genes influencing the myoD activity in SMS-CTR eRMS (SMS) and CW9019 aRMS (CW) cell lines cultured growth medium (GM).</p>
<p>Expression of miR-328, miR-338-3p and miR-485-5p in SMS-CTR eRMS (SMS) CW9019 aRMS (CW).</p>
<p>Growth of tumors formed by CW9019-Luc aRMS cells transplanted subcutaneously to athymic nude mice.</p>
<p>Effect of miR-206 on expression differentiation markers in SMS-CTR eRMS (SMS) and CW9019 aRMS (CW) cell lines.</p>