A5039508610- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Protist diversity and phylogeny
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Polar Research and Ecology
- Paleontology and Stratigraphy of Fossils
- Wastewater Treatment and Nitrogen Removal
- Membrane Separation Technologies
- Metabolomics and Mass Spectrometry Studies
- Powder Metallurgy Techniques and Materials
- Forest Ecology and Biodiversity Studies
- Advanced Proteomics Techniques and Applications
- Genetic Neurodegenerative Diseases
- Algal biology and biofuel production
- Food Chemistry and Fat Analysis
- Legume Nitrogen Fixing Symbiosis
- Coastal wetland ecosystem dynamics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Diatoms and Algae Research
- Plant nutrient uptake and metabolism
- Metabolism and Genetic Disorders
- Image Processing and 3D Reconstruction
- Planetary Science and Exploration
Swedish University of Agricultural Sciences
2013-2024
Apple (United States)
2024
Montana State University
2007-2020
Agricultural Research Service
2016-2018
United States Department of Agriculture
2016
University of Minnesota
2003-2016
Pennsylvania State University
2007
University of Southern California
2007
Only five bacterial phyla with members capable of chlorophyll (Chl)–based phototrophy are presently known. Metagenomic data from the phototrophic microbial mats alkaline siliceous hot springs in Yellowstone National Park revealed existence a distinctive bacteriochlorophyll (BChl)–synthesizing, bacterium. A highly enriched culture this bacterium grew photoheterotrophically, synthesized BChls and c under oxic conditions, had chlorosomes type 1 reaction centers. “ Candidatus Chloracidobacterium...
Microbial-mat communities in the effluent channels of Octopus and Mushroom Springs within Lower Geyser Basin at Yellowstone National Park have been studied for nearly 50 years. The emphasis has mostly focused on chlorophototrophic bacterial organisms phyla Cyanobacteria Chloroflexi. In contrast, diversity metabolic functions heterotrophic community microoxic/anoxic region mat are not well understood. this study we analyzed orange-colored undermat microbial Spring using metagenomic...
Abstract Background Three methods were developed for the application of stoichiometry-based network analysis approaches including elementary mode to study mass and energy flows in microbial communities. Each has distinct advantages disadvantages suitable analyzing systems with different degrees complexity a priori knowledge. These tested compared using data from thermophilic, phototrophic mat communities Octopus Mushroom Springs Yellowstone National Park (USA). The models based on three...
Stable carbon isotope signatures of diagnostic lipid biomarkers have suggested that Roseiflexus spp., the dominant filamentous anoxygenic phototrophic bacteria inhabiting microbial mats alkaline siliceous hot springs, may be capable fixing bicarbonate via 3-hydroxypropionate pathway, which has been characterized in their distant relative, Chloroflexus aurantiacus. The genomes three Chloroflexi isolates (Roseiflexus sp. RS-1, castenholzii and aggregans), but not a non-photosynthetic isolate...
Six phototrophic microbial mat communities from different geothermal springs (YNP) were studied using metagenome sequencing and geochemical analyses. The primary goals of this work to determine differences in community composition high-temperature mats distributed across the Yellowstone ecosystem, identify metabolic attributes predominant organisms present these that may correlate with environmental important niche differentiation. Random shotgun sequences six (average~ 53 Mbp/site)...
Roseiflexus sp. strains were cultivated from a microbial mat of an alkaline siliceous hot spring in Yellowstone National Park. These are closely related to predominant filamentous anoxygenic phototrophs found the mat, as judged by similarity small-subunit rRNA, lipid distributions, and genomic metagenomic sequences. Like Japanese isolate, R. castenholzii, isolates contain bacteriochlorophyll a, but not c or chlorosomes, grow photoheterotrophically chemoheterotrophically under dark aerobic...
Abstract Coffee is prepared by the extraction of a complex array organic molecules from roasted bean, which has been ground into fine particulates. The depends on temperature, water chemistry and also accessible surface area coffee. Here we investigate whether variations in production processes single origin coffee beans affects particle size distribution upon grinding. We find that independent bean processing method. Furthermore, elucidate influence temperature distribution, concluding...
Abstract Membrane‐coupled bioreactors (MBRs) offer many benefits compared to conventional biological wastewater treatment systems; however, their performance characteristics are poorly understood. Laboratory‐scale MBRs were used study bacterial adaptations in physiology and community structure. fed a mixture of starch, gelatin, polyoxyethylene‐sorbitan monooleate simulate the polysaccharide, protein, lipid components municipal wastewater. Physiological detected by measuring ectoenzyme...
Protein-stable isotope probing (protein-SIP) has strong potential for revealing key metabolizing taxa in complex microbial communities. While most protein-SIP work to date been performed under controlled laboratory conditions allow extensive labeling of the target organism(s), a application will be situ studies communities short periods time natural that result small degrees partial labeling. One hurdle restricting large-scale is lack algorithms and software automated data processing massive...
ABSTRACT A shotgun metaproteomics approach was employed to identify proteins in a hot spring microbial mat community. We identified 202 encompassing 19 known functions from 12 phyla. Importantly, we two key enzymes involved the 3-hydroxypropionate CO 2 fixation pathway uncultivated Roseiflexus spp., which are photoheterotrophs.
The upper green layer of the chlorophototrophic microbial mats associated with alkaline siliceous hot springs Yellowstone National Park consists oxygenic cyanobacteria (
Abstract Isotopic pulse-labelling of photosynthate allows tracing carbon (C) from tree canopies to belowground biota and calculations its turnover in roots recipient soil microorganisms. A high concentration label is desirable, but difficult achieve field studies intact ecosystem patches with trees. Moreover, root systems trees overlap considerably most forests, which requires a large labelled area minimize the impact C allocated by un-labelled We describe method, combines level labelling at...