A5054705052- Signaling Pathways in Disease
- Insect Resistance and Genetics
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Chemokine receptors and signaling
- Protein Structure and Dynamics
- Diet, Metabolism, and Disease
- Diabetes and associated disorders
- Proteoglycans and glycosaminoglycans research
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Metabolism, Diabetes, and Cancer
- Electrochemical Analysis and Applications
- Trace Elements in Health
- Protein purification and stability
- Analytical Chemistry and Chromatography
- Parathyroid Disorders and Treatments
- Cell Adhesion Molecules Research
- Antibiotic Resistance in Bacteria
- interferon and immune responses
- Ion-surface interactions and analysis
- Toxin Mechanisms and Immunotoxins
- Chromatography in Natural Products
- Biochemical effects in animals
- Microbial Metabolic Engineering and Bioproduction
Institut des Sciences Analytiques
2022-2024
St. Jude Children's Research Hospital
2023
Centre National de la Recherche Scientifique
2017-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2019
Université Grenoble Alpes
2015-2019
Institut de Biologie Structurale
2015-2019
CEA Grenoble
2017-2018
Institut Européen de Chimie et Biologie
2011-2017
Inserm
2017
Université de Bordeaux
2017
The chemokine receptor, CXC receptor 4 (CXCR4), is selective for ligand 12 (CXCL12), broadly expressed in blood and tissue cells, essential during embryogenesis hematopoiesis. CXCL14 a homeostatic with unknown selectivity preferential expression peripheral tissues. Here, we demonstrate that synergized CXCL12 the induction of responses primary human lymphoid cells cell lines express CXCR4. Combining subactive concentrations 100–300 nM resulted chemotaxis exceeded maximal were obtained alone....
Heparan sulfate (HS) is a linear, complex polysaccharide that modulates the biological activities of proteins through binding sites made by series Golgi-localized enzymes. Of these, glucuronyl C5-epimerase (Glce) catalyzes C5-epimerization HS component, d -glucuronic acid (GlcA), into l -iduronic (IdoA), which provides internal flexibility to polymer and forges protein-binding ensure function. Here we report crystal structures human Glce in unbound state an inactive mutant, as assessed...
Chemokines promote directional cell migration through binding to G-protein-coupled receptors, and as such are involved in a large array of developmental, homeostatic pathological processes. They also interact with heparan sulfate (HS), the functional consequences which depend on respective location receptor- HS-binding sites, detail that remains elusive for most chemokines. Here, set up biochemical framework investigate how HS can regulate CXCL13 activity, we solved solution structure...
The FK506-binding protein (FKBP) family of peptidyl-prolyl isomerases (PPIases) is characterized by a common catalytic domain that binds to the inhibitors FK506 and rapamycin. As one four FKBPs within yeast Saccharomyces cerevisiae, Fpr4 has been described as histone chaperone, in addition implicated epigenetic function part due its mediation cis-trans conversion proline residues tails. To better understand molecular details this activity, we have determined solution structure C-terminal...
The resistance of gram-negative bacteria to silver ions is mediated by a efflux pump, which mainly relies on tripartite complex SilCBA, metallochaperone SilF and an intrinsically disordered protein SilE. However, the precise mechanism are extruded from cell different roles SilB, SilF, SilE remain poorly understood. To address these questions, we employed nuclear magnetic resonance mass spectrometry investigate interplay between proteins. We first solved solution structures in its free...
The nucleoplasmin family of histone chaperones is identified by a pentamer-forming domain and multiple acidic tracts that mediate binding chaperone activity. Within this family, novel organization was recently discovered consists an N-terminal nucleoplasmin-like (NPL) C-terminal FKBP peptidyl-proline isomerase domain. Saccharomyces cerevisiae Fpr4 one such protein. Here we report in addition to its known prolyl activities, the binds nucleosomes nucleosome arrays vitro. This ability mediated...
Silver has been used for its antimicrobial properties to fight infection thousands of years. Unfortunately, some Gram-negative bacteria have developed silver resistance causing the death patients in a burn unit. The genes responsible designated as sil operon. Among proteins operon, SilE shown play key role bacterial resistance. Based on limited information available, it depicted an intrinsically disordered protein that folds into helices upon ion binding. Herein, this work demonstrates is...
A common separation approach for polar compounds involves coupling reversed-phase liquid chromatography (RPLC) with hydrophilic interaction (HILIC) in two-dimensional chromatography. The higher proportion of acetonitrile used the HILIC mobile phase, which enhances mass spectrometry detection, encourages its use second dimension. Previous studies demonstrated that column can be partially equilibrated within very short timeframes without compromising retention time stability, rendering it...