A5080091171- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Ion Channels and Receptors
- Diet and metabolism studies
- Multisensory perception and integration
- Nutritional Studies and Diet
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Dietary Effects on Health
- Agriculture Sustainability and Environmental Impact
- Neural dynamics and brain function
- Nutrition, Genetics, and Disease
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- Neural and Behavioral Psychology Studies
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Lipid Membrane Structure and Behavior
- Circadian rhythm and melatonin
- Bacterial Genetics and Biotechnology
- Receptor Mechanisms and Signaling
- Piperaceae Chemical and Biological Studies
- Biofuel production and bioconversion
- Global Public Health Policies and Epidemiology
UNSW Sydney
2021-2025
Australian Human Rights Commission
2023-2025
Nestlé (Switzerland)
2007-2019
Imperial College London
2019
The University of Tokyo
2010-2016
École Polytechnique Fédérale de Lausanne
2015
Hôpital Nestlé
2014
Nestlé (Brazil)
2009-2013
Nestlé (United Kingdom)
2010
Centre des Sciences du Goût et de l'Alimentation
2009
The oral perception of fat has traditionally been considered to rely mainly on texture and olfaction, but recent findings suggest that taste may also play a role in the detection long chain fatty acids. two G-protein coupled receptors GPR40 (Ffar1) GPR120 are activated by medium Here we show expressed buds, type II I cells, respectively. Compared with wild-type mice, male female knock-out mice diminished preference for linoleic acid oleic acid, nerve responses several These results mediate
The taste system, made up of receptor cells clustered in buds at the surface tongue and soft palate, plays a key role decision to ingest or reject food thereby is essential protecting organisms against harmful toxins selecting most appropriate nutrients. To determine if similar chemosensory system exists gastrointestinal tract, we used immunohistochemistry real-time polymerase chain reaction (PCR) investigate which taste-signaling molecules are expressed intestinal mucosa. PCR data showed...
Abstract To determine the role in chemosensation of intestinal solitary cells that express taste receptors and Trpm5, we carried out a microarray study transcriptome FACS‐sorted transgenic mouse expressing enhanced green fluorescent protein (eGFP) under control Trpm5 promoter compared it with do not eGFP. The findings are: 1) Morphology expression markers show most eGFP+ are brush cells. 2) majority proteins known to be involved signal transduction expressed cells, although isoforms always...
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts characteristic flavor cinnamon and known be main agonist of transient receptor potential-ankyrin 1 (TRPA1). Here, expression TRPA1 in epithelial mouse stomach cells described. After receiving a single-dose CIN, mice significantly reduce cumulative food intake gastric emptying rates. Co-localization ghrelin enteroendocrine duodenum observed both vivo...
Background and purpose: Oily extracts of Sichuan Melegueta peppers evoke pungent sensations mediated by different alkylamides [mainly hydroxy‐α‐sanshool (α‐SOH)] hydroxyarylalkanones (6‐shogaol 6‐paradol). We assessed how transient receptor potential ankyrin 1 (TRPA1) TRP vanilloid (TRPV1), two chemosensory ion channels, participate in these sensations. Experimental approach: The structure–activity relationships molecules on TRPA1 TRPV1 was measured testing natural synthetic analogues using...
Throughout the world many people use artificial sweeteners (AS) for purpose of reducing caloric intake. The most prominently used these molecules include saccharin, aspartame (Nutrasweet), acesulfame-K, and cyclamate. Despite advantage they provide, one key concern in their is aversive aftertaste that has been characterized on a sensory level as bitter and/or metallic. Recently, it shown activation particular T2R taste receptors partially involved with sensation saccharin acesulfame-K. To...
The circadian clock in peripheral tissues can be entrained by restricted feeding (RF), a regimen that restricts the duration of food availability with no calorie restriction (CR). However, it is not known whether RF delay occurrence age-associated changes similar to CR. We measured expression genes, disease marker metabolic factors and inflammatory allergy markers mouse serum, liver, jejunum white adipose tissue (WAT) after long-term 4 months. found rhythmicity more robust phase advanced...
FIELD GRAND CHALLENGE article Front. Nutr., 20 March 2014 https://doi.org/10.3389/fnut.2014.00001
Metabolic traits are molecular phenotypes that can drive clinical and may predict disease progression. Here, we report results from a metabolome- genome-wide association study on 1H-NMR urine metabolic profiles. The was conducted within an untargeted approach, employing novel method for compound identification. From our discovery cohort of 835 Caucasian individuals who participated in the CoLaus study, identified 139 suggestively significant (P<5×10−8) independent associations between single...
Genes encoding membrane proteins comprise a substantial proportion of genomes sequenced to date, but ability perform structural studies on this portion the proteome is limited. Electrospray ionization-MS (ESI-MS) an intact protein generates profile defining native covalent state gene product and its heterogeneity. Here we apply ESI-MS technology with accuracy exceeding 0.01% hydrophobic 12-transmembrane α-helices, full-length lactose permease from Escherichia coli . Furthermore, used titrate...
Vision provides a primary sensory input for food perception. It raises expectations on taste and nutritional value drives acceptance or rejection. So far, the impact of visual cues varying in energy content subsequent integration remains unexplored. Using electrical neuroimaging, we assessed whether high- low-calorie differentially influence brain processing perception neutral electric taste. When viewing high-calorie images, participants reported to be more pleasant than when images...
Experimental evidence for proton transfer via a hydrogen-bonded network in membrane protein is presented. Bacteriorhodopsin's mechanism on the uptake pathway between Asp-96 and Schiff base M-to-N transition was determined. The slowdown of this by removal donor Asp-96-->Asn mutant can be accelerated again addition small weak acid anions such as azide. Fourier-transform infrared experiments show transient protonation azide bound to and, due azide, restoration IR continuum band changes seen...
A general technique has been developed that allows rapid mass spectrometric analysis of full-length membrane proteins [Whitelegge, J. P., le Coutre, J., et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 10695−10698]. Using in-line HPLC electrospray ionization spectrometry (LC−MS), different native and recombinant bacterial up to 61 kDa are characterized. Mass data four entirely from three organisms, two transporters, a channel, porin protein presented. In addition determination the molecular...
The Na<sup>+</sup>/galactose cotransporter (vSGLT) of <i>Vibrio parahaemolyticus</i>, tagged with C-terminal hexahistidine, has been purified to apparent homogeneity by Ni<sup>2+</sup> affinity chromatography and gel filtration. Resequencing the <i>vSGLT</i> gene identified an important correction: N terminus constitutes additional 13 functionally essential residues. mass His-tagged vSGLT expressed under its native promoter, as determined electrospray ionization-mass spectrometry (ESI-MS),...
Natural genetic variation can have a pronounced influence on human taste perception, which in turn may food preference and dietary choice. Genome-wide association studies represent powerful tool to understand this influence. To help optimize the design of future genome-wide-association perception we used well-known TAS2R38-PROP as determine relative power efficiency different phenotyping data-analysis strategies. The results show that choice both data collection processing schemes very...
Human perception of bitterness displays pronounced interindividual variation. This phenotypic variation is mirrored by equally genetic in the family bitter taste receptor genes. To better understand effects common variations on human perception, we conducted a genome-wide association study discovery panel 504 subjects and validation 104 from general population São Paulo Brazil. Correction for taste-sensitivity allowed us to identify SNP cluster receptors chr12 (10.88– 11.24 Mb, build 36.1)...
Active ingredients of spices (AIS) modulate neural response in the peripheral nervous system, mainly through interaction with TRP channel/receptors. The present study explores how different AIS layer 5 pyramidal neurons S1 neocortex. tested are agonists TRPV1/3, TRPM8 or TRPA1. Our results demonstrate that capsaicin, eugenol, menthol, icilin and cinnamaldehyde, but not AITC dampen generation APs a voltage- time-dependent manner. This effect was further for ligands presence blocker (BCTC) on...
Abstract Various lines of published evidence have already demonstrated the impact TRPV1 agonists on energetic metabolism through stimulation sympathetic nervous system (SNS). This study presents a trial investigating if two related sensory receptors TRPA1 and TRPM8 could also stimulate SNS healthy subjects. The was designed to be double-blinded, randomized, cross-over, placebo-controlled with subjects autonomic (ANS) cinnamaldehyde, capsaicin cooling flavor measured during 90 min after...
Cellular agriculture, an emerging technology, aims to produce animal-based products such as meat through scalable tissue culture methods. Traditional techniques rely on chemically undefined media using fetal bovine serum (FBS) or defined utilizing specific growth factors. To be a viable alternative conventional production, cellular agriculture requires cost-effective materials with established supply chains for media. Here, we investigate hydrolysates from Kikuyu grass, Alfalfa and cattle...
The advancement of cellular agriculture hinges on replicating the mouthfeel, taste, and texture conventional meat, which are largely determined by fat tissue composed adipocytes. However, growing cells at scale remains a significant challenge for field. This study explores use edible bioscaffolds to support large-scale production bovine Scaffold-based approaches commonly used facilitate proliferation adherent within bioreactors, yet identifying suitable, scaffolds cultured meat an ongoing...
The structure of lactose permease from Escherichia coli in its lipid environment was studied by attenuated total reflection Fourier transform infrared spectroscopy. protein exhibits an α-helical content about 65% and 25% β-sheet. Unusually fast hydrogen/deuterium (H/D) exchange to 90–95% completion suggests a that is highly accessible the aqueous phase. An average tilt angle 33° for helices found with respect bilayer normal at lipid-to-protein ratio ≈800:1 (mol/mol), optimal activity under...