A5076656358- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Glioma Diagnosis and Treatment
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- Cancer Treatment and Pharmacology
- Advanced Biosensing Techniques and Applications
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Brain Metastases and Treatment
- Advanced Proteomics Techniques and Applications
- Enzyme Structure and Function
- Lung Cancer Research Studies
- Fluorine in Organic Chemistry
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Catalytic Cross-Coupling Reactions
- Bacterial Genetics and Biotechnology
Vanquish Oncology (United States)
2015-2023
University of Illinois Urbana-Champaign
2023
Arizona Oncology
2020
Tethys Research (United States)
2008-2011
Lawrence Livermore National Laboratory
2006-2009
Teledyne Technologies (United States)
2009
North Carolina State University
2004
Nivalis Therapeutics (United States)
1997-2000
University of Utah
1997
University of Colorado Boulder
1994
Aptamers are nucleic acid molecules that have been selected in vitro to bind their molecular targets with high affinity and specificity. Typically, the systematic evolution of ligands by exponential enrichment (SELEX) process is used for isolation specific, high-affinity aptamers. SELEX, however, an iterative requiring multiple rounds selection amplification demand significant time labor. Here, we describe aptamer discovery system rapid, highly efficient, automatable, applicable a wide range...
Abstract Biomanufacturing could contribute as much ${\$}$30 trillion to the global economy by 2030. However, success of growing bioeconomy depends on our ability manufacture high-performing strains in a time- and cost-effective manner. The Design–Build–Test–Learn (DBTL) framework has proven be an effective strain engineering approach. Significant improvements have been made genome engineering, genotyping, phenotyping throughput over last couple decades that greatly accelerated DBTL cycles....
Activation of the unfolded protein response induces regression estrogen receptor α–positive breast cancer xenografts in mice.
Attachment of a Ni(II)-glyglyhis chelate to trifluoperazine results in molecule capable cleaving calmodulin at single locus upon activation with peracid.
Optimization of high affinity reagents is a significant bottleneck in medicine and the life sciences. The ability to synthetically create thousands permutations lead high-affinity reagent survey properties individual parallel could potentially relieve this bottleneck. Aptamers are single stranded oligonucleotides isolated by vitro selection processes as class have been shown bind wide variety target molecules.High density DNA microarray technology was used synthesize, situ, arrays...
RNA molecules that bind a transition state analog for Diels-Alder reaction (Kd = 0.35 +/- 0.05 mM) were isolated from starting pool of approximately 10(14) sequences by affinity chromatography. After the initial rise and plateau amount eluted with soluble analog, step gradient elution was used to further enrich higher affinities target. To our knowledge, isolation either nonplanar or hydrophobic ligand has not been reported previously. A conserved nucleotide sequence secondary structure...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of (1R,2S)- and (1S,2R)-2-chloro-1,2-diphenylethanol other .beta.-halohydrins in enantiomerically pure formJoseph P. Konopelski, Mark A. Boehler, Theodore M. TarasowCite this: J. Org. Chem. 1989, 54, 20, 4966–4970Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/jo00281a049https://doi.org/10.1021/jo00281a049research-articleACS...
Abstract Background The immune system undergoes a myriad of changes with age. While it is known that antibody-secreting plasma and long-lived memory B cells change age, remains unclear how the binding profile circulating antibody repertoire impacted. Results To understand humoral immunity respect to we characterized serum high density peptide microarrays in diverse cohort 1675 donors. We discovered thousands peptides bind antibodies age-dependent fashion, many which contain di-serine motifs....
The active site components and substrate specificity of an RNA Diels−Alderase (DA22) were investigated. activity was found to be highly dependent on a unique 5-position pyridyl modified uridine. Even closely related modifications failed yield catalysts. Substrate this remarkable. Experiments with alternative diene dienophile substrates showed the discriminating, even against molecules similar reactivity structure. Inhibition studies series product analogues established that recognizes...
Studies were undertaken to explore the application of tandem mass spectrometry for structure analysis unfractionated mixtures oligonucleotides. Limited combinatorial libraries constructed 8-mers (NGACACNG; nine compounds) and 12-mers (NGACTNAGACNG; 27 compounds), where N is any 2'-deoxyribonucleotides uracil, thymine, or 5-[N-(aminoethyl)-3-acrylimido]uracil. Molecular measurements mixture components (single analyzer) in simplest cases acquisition collision-induced dissociation spectra...
Enzymes can substantially increase the probability of a reaction by exploiting binding energy to preorganize their substrates into reactive conformations. Similar effects are likely be important in wide variety designed catalysts, including catalytic antibodies. Transferred nuclear Overhauser have been used here investigate how an antibody possessing chorismate mutase activity binds its flexible substrate molecule chorismate. The conversion prephenate way Claisen rearrangement requires adopt...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInvestigation of Medium Effects in a Family Decarboxylase AntibodiesTheodore M. Tarasow, Cristina Lewis, and Donald HilvertCite this: J. Am. Chem. Soc. 1994, 116, 18, 7959–7963Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://pubs.acs.org/doi/10.1021/ja00097a001https://doi.org/10.1021/ja00097a001research-articleACS PublicationsRequest reuse permissionsArticle...
A group of eight unique RNA sequences isolated from a single in vitro selection experiment were characterized based on their Diels−Alderase properties. Five the isolates contain 10-base conserved region, while remaining three do not share any sequence similarities with others. This series Diels−Alderases displayed kcat and Km values that spanned 74 80-fold ranges, respectively. These appeared to compensate for one another, as value kcat/Km varied by only 5-fold among studied. Product...
Background The complexity of the eukaryotic parasite Trypanosoma (T.) cruzi manifests in its highly dynamic genome, multi-host life cycle, progressive morphologies and immune-evasion mechanisms. Accurate determination infection or Chagas' disease activity prognosis continues to challenge researchers. We hypothesized that a diagnostic platform with higher ligand than previously employed may hold value. Methodology applied ImmunoSignature Technology (IST) for detection T. cruzi-specific...
Abstract Background Procaspase-3 (PC-3) is overexpressed in various tumor types, including gliomas. Targeted PC-3 activation combined with chemotherapy a novel strategy for treating patients high-grade gliomas, promising preclinical activity. This study aimed to define safety and tolerability of procaspase-activating compound-1 (PAC-1) combination temozolomide (TMZ) recurrent astrocytomas. Methods A modified-Fibonacci dose-escalation 3 + design was used. PAC-1 administered at increasing dose...
In this manuscript the catalytic ability of RNA is examined and compared to other biopolymers. Despite having considerably fewer catalytically enabling properties when proteins, power in vitro selection has allowed for DNA catalysts be isolated. catalysis been expanded by incorporating modified bases enrich structural functional diversity RNA. Successful examples new chemistry using base modifications include carbon–carbon bond forming reactions creation highly specific active sites that are...
Chemical mutagenesis of a previously reported RNA Diels-Alderase (DA22) was followed by in vitro selection based on [4 + 2] catalysis. New mutated families Diels-Alderases closely related sequence space were obtained. The selected showed significant improvements catalytic efficiency (k(cat)/K(m)) as compared to the original DA22. improvement activity primarily due decrease K(m), but modest increases k(cat) also observed. increase these new found not negatively affect their dienophile...
An efficient nickel-catalyzed Kumada-Corriu cross coupling enabled the introduction of an alpha-fluorovinyl functionality with excellent conversion and specificity.