A5017255197- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- T-cell and Retrovirus Studies
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Vector-Borne Animal Diseases
- Microbial infections and disease research
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Veterinary Equine Medical Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Zoonotic diseases and public health
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Allergic Rhinitis and Sensitization
- Veterinary Oncology Research
- Viral Infections and Immunology Research
- Cytomegalovirus and herpesvirus research
- Reproductive Physiology in Livestock
- Bacillus and Francisella bacterial research
- Glycosylation and Glycoproteins Research
Animal Health Trust
1999-2019
University of Nottingham
1982-2019
Loughborough University
2016-2019
Cyprus Institute of Neurology and Genetics
2016
Leibniz Institute for Zoo and Wildlife Research
2016
Freie Universität Berlin
2016
Suffolk University
2013
Institute of Occupational Medicine
1985-1988
Cancer Research UK
1982
University of Cambridge
1982
The final assignment of antibody clusters for leucocyte antigens and immunoglobulins, as described in detail Sections 3 4, is summarized Table 4. Together with other mAbs developed outside ELAW II (Table 9) this pool reagents represent a powerful array tools the study equine immunity. Second Equine Leucocyte Antigen Workshop made considerable advances pursuing objectives establishing specificities achieving consensus on nomenclature immunoglobulin molecules. Of equal importance, several...
Summary Twelve adult ponies and 2 conventional foals were exposed intranasal to EHV‐1, strain Ab4 (TCID 50 10 −6.6 ) samples of respiratory tract associated lymphoid tissues recovered between 12 h 13 days after infection. Infectious virus was detected in tissue homogenates using susceptible cell monolayers expression viral antigens monitored indirect immunoperoxidase histochemistry on paraffin sections. The results showed both infectious EHV‐1 lymph nodes exposure. Infected leucocytes...
Equine influenza vaccines containing inactivated whole virus and Carbomer adjuvant stimulated higher levels longer lasting antibody to haemagglutinin in ponies than of equivalent antigenic content aluminium phosphate adjuvants. Five months after the third dose vaccine adjuvant, were protected against clinical disease induced by an aerosol virulent (A/equine/Newmarket/79, H3N8). In contrast which received susceptible infection disease. There was inverse correlation between prechallenge...
Summary From 1988 to 1991, 51 pregnant pony mares were challenged intranasally or by aerosol with an isolate of EHV‐1 (AB4) originally recovered from a quadriplegic mare. This resulted in 32 abortions, occurring 9 29 days after infection. In 14 the early abortions (Days 9–14), was not demonstrated foetal tissues virus isolation immunostaining despite no other non‐viral cause for abortion being evident. Application polymerase chain reaction these cases also proved negative. One destroyed...
Summary Infection of seronegative Welsh mountain ponies was established by intranasal instillation or exposure to nebulised aerosol egg grown H3N8 viruses. Pyrexia and coughing were noted following high titres virus recovered from the nasopharynx. Exposure resulted in more severe clinical signs characterised temperatures, dyspnoea, anorexia coughing; lower levels The severity kinetics shedding dose‐related with minimal infectious dose being 10 2 EID 50 /ml when exposed aerosols produced...
The duration of immunity as measured by virological, serological and clinical responses following infection with influenza A/equine/Newmarket/79 (H3N8) was assessed in repeated challenge experiments which ponies were infected exposure to aerosols infectious virus. Previous stimulated complete protection persisted for at least 32 weeks demonstrated the absence febrile coughing two groups 16 or after first infection. Partial over a year reduction number group 62 their These results contrasted...
Field outbreaks of influenza that occurred in vaccinated Thoroughbred racehorses Newmarket 1995 and 1996 were investigated by nucleoprotein ELISA serology. Investigations showed serum levels vaccine-induced single radial haemolysis (SRH) antibody correlated closely with protective immunity against equine consistent observations made previous experimental studies using nebulised aerosol challenge. In the second part this study, stimulated vaccination to examine probable protection high risk...
Summary Twelve adult ponies and 2 conventional foals were exposed to 10 6.6 TCID 50 of Equid herpesvirus‐1 (EHV‐1), strain Ab4 samples respiratory tract tissues recovered. Infectious virus in tissue homogenates was detected using susceptible cell monolayers expression viral antigens monitored indirect immunoperoxidase histochemistry paraffin sections. The results illustrated the rapid dissemination EHV‐1 throughout tract, with early replication lungs one day after exposure. Endothelial...
Seven previously untreated five-month-old New Forest ponies received two doses of equine influenza immunostimulating complex vaccines, one with and without an immunopurified tetanus toxoid component, given by deep intramuscular injection six weeks apart, followed a booster dose five months later. Fifteen after the third vaccine, were challenged exposure to aerosol A/Equine 2/Sussex/89 (H3N8), virus isolated from recent outbreak A/equine 2 in Britain. The challenge produced severe clinical...
West Nile Virus (WNV) is the causative agent of a vector-borne, zoonotic disease with worldwide distribution. Recent expansion and introduction WNV into new areas, including southern Europe, has been associated severe in humans equids, increased concerns regarding need to prevent control future outbreaks. Since 2010, 524 confirmed human cases have reported Greece greater than 10% mortality. Infected mosquitoes, wild birds, chickens detected disease. The aim our study was establish monitoring...
In July 1989 influenza A/equine-2 (H3N8) was isolated from a nasopharyngeal swab taken non-thoroughbred horse exhibiting acute clinical respiratory disease. This the first isolation of equine virus in United Kingdom since 1981. Subsequent investigations disease horses indicated that infection dispersed throughout UK. However, unlike previous epidemic 1979, which had been vaccinated. outbreak provided an opportunity to evaluate antigen capture ELISA, directed against nucleoprotein, as rapid...
Twelve Welsh Mountain pony mares in late gestation were infected intranasally with EHV-1 (AB4 isolate) at dose rates from 10(3) to 10(7.3) TCID50. This resulted 3 cases of paresis, Days 9, 10 and 12 after inoculation, 5 abortions, 6, 18, 19 20. Euthanasia was performed between 6 21, collection uterine specimens for histopathology, virus isolation immunoperoxidase staining the pregnant horn, non-pregnant horn body. replication endometrial vessels detected as early Day maximal 9-11, when...
Abstract Objective —To estimate the heritability of atopic dermatitis in Golden and Labrador Retrievers. Animals —429 dogs related to 13 with dermatitis. Procedure —Atopic was defined on basis type frequency clinical signs recorded records, each dog classified or probable as nonatopic. By use data from nonatopic dogs, regression analyses parental status offspring were performed heritability. Results —There no difference between sexes breeds. There a marked association parent that offspring,...