A5081632559- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Banana Cultivation and Research
- Plant Micronutrient Interactions and Effects
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Vitamin C and Antioxidants Research
- Plant tissue culture and regeneration
- Cassava research and cyanide
- Plant and soil sciences
- Synthesis and biological activity
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Melanoma and MAPK Pathways
- Microbial Metabolic Engineering and Bioproduction
- Agricultural and Food Sciences
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- Plant Pathogens and Resistance
- Biodiesel Production and Applications
- Malaria Research and Control
- Growth and nutrition in plants
- Gene expression and cancer classification
- Biochemical and biochemical processes
- Tuberculosis Research and Epidemiology
Universidad Nacional de la Amazonía Peruana
2015-2024
Ecopetrol (Colombia)
2024
University of Córdoba
2024
Daniel Alcides Carrión National University
2023
Pontificia Universidad Católica de Chile
2021
Universidad Técnica de Babahoyo
2021
CIUDAD
2019
Instituto de Investigaciones de la Amazonía Peruana
2019
Georgia Institute of Technology
2018
Karolinska Institutet
1993-2010
The p53 protein activates cellular death programs through multiple pathways. Because the high frequency of mutations in human tumors is believed to contribute resistance commonly used chemotherapeutic agents, it important identify drugs that induce p53-independent cell and define mechanisms action such drugs. Here we screened a drug library (the National Cancer Institute mechanistic set; 879 compounds with diverse actions) identified 175 induced caspase cleavage cytokeratin-18 cultured...
The most common practice in studying and cataloguing prokaryotic diversity involves the grouping of sequences into operational taxonomic units (OTUs) at 97% 16S rRNA gene sequence identity level, often using partial sequences, such as PCR-generated amplicons. Due to high conservation genes, organisms belonging closely related yet distinct species may be grouped under same OTU. However, it remains unclear how much has been underestimated by this practice. To address question, we compared OTUs...
Abstract The majority of human melanomas harbor activating mutations in either the BRAF or NRAS gene. To date, role oncogenic melanoma remains poorly defined and no current therapies are directed at specifically suppressing tumors. aim our study, therefore, was to investigate effects cell lines vitro . Using both small interfering RNA‐ plasmid based‐RNA interference techniques, suppressed 2 lines, 224 BL, which a codon 61 CAA (glutamine) CGA (arginine) mutation. Suppression these resulted...
Aberrant activation of the MAPK pathway drives cell proliferation in multiple cancers. Inhibitors BRAF and MEK kinases are approved for treatment mutant melanoma, but resistance frequently emerges, often mediated by increased signaling through ERK1/2. Here, we describe fragment-based generation ERK1/2 inhibitors that block catalytic phosphorylation downstream substrates such as RSK also modulate without directly inhibiting MEK. X-ray crystallographic biophysical fragment screening followed...
Background: Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in biosynthesis and accumulation among different cultivars present challenges commercial production. This study aimed establish an efficient vitro regeneration protocol Vitahuayo cultivar through callus induction somatic embryogenesis. Methods: Stem leaf explants were cultured on Murashige Skoog (MS) medium supplemented with 24-dichlorophenoxyacetic...
Plasmodium falciparum entered into the Peruvian Amazon in 1994, sparking an epidemic between 1995 and 1998. Since 2000, there has been sustained low P. transmission. The Malaria Immunology Genetics project longitudinally followed members of community Zungarococha (N = 1,945, 4 villages) with active household health center-based visits each year since 2003. We examined parasite population structure traced genetic diversity temporally spatially. genotyped infections over 5 years (2003–2007)...
Biodiesel production from microalgae triacylglycerols is growing, because this feedstock a more sustainable and advantageous alternative. In study, we isolated identified fourteen strains of native the Peruvian Amazon. These showed great heterogeneity in biomass productivity, lipid productivity content, thus, three them (Acutodesmus obliquus, Ankistrodesmus sp. Chlorella lewinii) were selected for further evaluation under culture nitrogen-sufficient (+N) nitrogen-deficient (−N) Chu medium...
ABSTRACT The contamination of aquatic environments by microplastic has become a major threat to biodiversity. presence is documented in the fauna oceans, but, Amazon basin, reports on occurrence are few. present study surveyed fishes an area Peruvian Amazon. We sampled 61 specimens 15 commercial species from local markets city Iquitos, Loreto Department. detected total 2337 particles, 1096 gills and 1241 internal organs (esophagus, stomach, intestine, liver, gonads, pancreas, swim bladder...
Abstract An easy method for preparation of bare cell nuclei from fresh solid tissues DNA flow cytometry is described. Pieces up to 2 × mm 3 size were fixed in formalin. After removal formalin by washing with ethanol and rehydration tap water, the tissue pieces incubated subtilisin Carlsberg (pronase, Sigma protease XXIV) then stained directly DAPI. Staining ethidium bromide gave unsatisfactory results. Neither mechanical disaggregation nor centrifugation used. The resulting nucleus...
Abstract l-Ascorbic acid (AsA, vitamin C) is a pivotal dietary nutrient with multifaceted importance in living organisms. In plants, the Smirnoff–Wheeler pathway primary route for AsA biosynthesis, and understanding mechanistic details behind its component enzymes has implications plant biology, nutritional science, biotechnology. As part of an initiative to determine structures all six core pathway, present study focuses on three them model species Myrciaria dubia (camu-camu): GDP-d-mannose...
Myrciaria dubia is an Amazonian fruit shrub that produces numerous bioactive phytochemicals, but best known by its high L-ascorbic acid (AsA) content in fruits. Pronounced variation AsA has been observed both within and among individuals, the genetic factors responsible for this are largely unknown. The goals of research, therefore, were to assemble, characterize, annotate transcriptome M. order reconstruct metabolic pathways determine if multiple contribute biosynthesis. In total 24,551,882...
Accurate detection of target microbial species in metagenomic datasets from environmental samples remains limited because the limit current methods is typically inaccessible and frequency false-positives, resulting inadequate identification regions genome that are either too highly conserved to be diagnostic (e.g., rRNA genes) or prone frequent horizontal genetic exchange mobile elements) unknown. To overcome these limitations, we introduce imGLAD, which aims detect (target) genomic...
The MAPK signaling pathway is commonly upregulated in human cancers. As the primary downstream effector of pathway, ERK an attractive therapeutic target for treatment MAPK-activated cancers and overcoming resistance to upstream inhibition. ASTX029 a highly potent selective dual-mechanism inhibitor, discovered using fragment-based drug design. Because its distinctive ERK-binding mode, inhibits both catalytic activity phosphorylation itself by MEK, despite not directly inhibiting MEK activity....
Malaria remains a global health challenge, particularly in Peru's Loreto region. Despite ongoing efforts, high infection rates and asymptomatic cases perpetuate transmission. The Peruvian Ministry of Health's "Zero Plan" targets elimination. This novel study combines microscopic, molecular, serological techniques to assess transmission intensity, identify epidemiological risk factors, characterize species-specific patterns across villages. findings aim inform targeted interventions support...
Abstract Objectives: Syndecan‐1 is a transmembrane proteoglycan involved in various biological processes. Its extracellular, and cytoplasmic domains may all participate signal transduction. The aim of this study was to investigate the roles these syndecan‐1. Materials methods: We transfected cells two mesenchymal tumour cell lines with full‐length syndecan‐1 construct three truncated variants, namely 78 lacking EC domain exception DRKE sequence; 77 extracellular whole RMKKK corresponding...
In plants, it is well-known that ascorbic acid (vitamin C) can be synthesized via multiple metabolic pathways but there still much to learned concerning their integration and control mechanisms. Furthermore, the structural biology of component enzymes has been poorly exploited. Here we describe first crystal structure for an L-galactose dehydrogenase [Spinacia oleracea GDH (SoGDH) from spinach], D-mannose/L-galactose (Smirnoff-Wheeler) pathway which converts into L-galactono-1,4-lactone. The...
In Tanzania, the International Working Formulation [WF] rather than WHO Classification is still being used in diagnosing malignant lymphomas (ML) and biological characterization including HIV/EBV association sketchy, thus restraining comparison, prognostication application of established therapeutic protocols. Archival, diagnostic ML biopsies (N = 336), available sera 35) screened by ELISA for HIV antibodies corresponding clinical/histological reports at Muhimbili National Hospital (MNH)...