A5026049586- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Lignin and Wood Chemistry
- Biochemical and biochemical processes
- Enzyme-mediated dye degradation
- Catalysis for Biomass Conversion
- Enzyme Catalysis and Immobilization
- biodegradable polymer synthesis and properties
- Fungal Biology and Applications
- Polymer composites and self-healing
- Microplastics and Plastic Pollution
- Enzyme Production and Characterization
- Process Optimization and Integration
- Recycling and Waste Management Techniques
- Fermentation and Sensory Analysis
- Potato Plant Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Carbon dioxide utilization in catalysis
- Adsorption and biosorption for pollutant removal
- Analytical Chemistry and Chromatography
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Particle Dynamics in Fluid Flows
- Anaerobic Digestion and Biogas Production
- Epoxy Resin Curing Processes
National Renewable Energy Laboratory
2016-2025
Oak Ridge National Laboratory
2020
Biotrend (Portugal)
2017
Biocant
2017
Lund University
2017
Chalmers University of Technology
2017
University of Aveiro
2017
Queensland University of Technology
2017
Texas A&M University – Commerce
2017
Zero to Three
2016
Lignin Consolidated Bioprocessing utilizes microbes that simultaneously depolymerize lignin and convert the resulting aromatic compounds to fuel chemical precursors.
The conversion of biomass-derived sugars and aromatic molecules to cis,cis-muconic acid (referred hereafter as muconic or muconate) has been recent interest owing its facile adipic acid, an important commodity chemical. Metabolic routes produce muconate from both many lignin-derived compounds require the use a decarboxylase convert protocatechuate (PCA, 3,4-dihydroxybenzoate) catechol (1,2-dihydroxybenzene), two central intermediates in this pathway. Several studies have identified PCA...
Summary Microbial conversion offers a promising strategy for overcoming the intrinsic heterogeneity of plant biopolymer, lignin. Soil microbes that natively harbour aromatic‐catabolic pathways are natural choices chassis strains, and Pseudomonas putida KT 2440 has emerged as viable whole‐cell biocatalyst funnelling lignin‐derived compounds to value‐added products, including its native carbon storage product, medium‐chain‐length polyhydroxyalkanoates ( mcl ‐ PHA ). In this work, series...
Significance White-rot fungi play an essential role in global carbon cycling because of their extraordinary ability to extracellularly deconstruct lignin, a recalcitrant plant biopolymer. Despite this, the intracellular metabolism lignin-deconstruction products by this fungal group has been largely overlooked, potentially due lack genetic tools and challenging growth-tracking protocols, which complicate studies that are considered routine other organisms. Here, we definitively demonstrate...
Poly(ethylene terephthalate) (PET) is the most abundantly consumed synthetic polyester and accordingly a major source of plastic waste. The development chemocatalytic approaches for PET depolymerization to monomers offers new options open-loop upcycling PET, which can leverage biological transformations higher-value products. To that end, here we perform four sequential metabolic engineering efforts in Pseudomonas putida KT2440 enable conversion glycolysis products via: (i) ethylene glycol...
Abstract Muconic acid is a bioprivileged molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this study, Pseudomonas putida KT2440 engineered to convert glucose xylose, the primary carbohydrates in lignocellulosic hydrolysates, muconic using model-guided strategy maximize theoretical yield. Using adaptive laboratory evolution (ALE) metabolic engineering strain express D-xylose isomerase pathway, we...
Enzymatic depolymerization of poly(ethylene terephthalate) (PET) has emerged as a potential method for PET recycling, but extensive thermomechanical preprocessing to reduce both the crystallinity and particle size is often conducted, which costly energy-intensive. In current work, we use high-crystallinity (HC-PET) low-crystallinity cryomilled (CM-PET) with three distinct distributions investigate effect on performance variant leaf compost-cutinase enzyme (LCC-ICCG). We show that LCC-ICCG...
<italic>cis</italic>,<italic>cis</italic>-Muconic acid for downstream separation and catalytic upgrading to adipic nylon-6,6 polymerization.
This work shows parallel strain and bioreactor process development to improve muconic acid production from aromatic compounds lignin.
Co-production of chemicals from lignocellulosic biomass alongside fuels holds promise for improving the economic outlook integrated biorefineries. In current biochemical conversion processes that use thermochemical pretreatment and enzymatic hydrolysis, fractionation hemicellulose-derived cellulose-derived sugar streams is possible using hydrothermal or dilute acid (DAP), which then offers a route to parallel trains fuel chemical production xylose- glucose-enriched streams. Succinic (SA)...
Biorefinery process development relies on techno-economic analysis (TEA) to identify primary cost drivers, prioritize research directions, and mitigate technical risk for scale-up through of detailed designs. Here, we conduct TEA a model 2000 dry metric ton-per-day lignocellulosic biorefinery that employs two-step pretreatment enzymatic hydrolysis produce biomass-derived sugars, followed by biological lipid production, recovery, catalytic hydrotreating renewable diesel blendstock (RDB). On...
Lignin valorization offers significant potential to enhance the economic viability of lignocellulosic biorefineries. However, because its heterogeneous and recalcitrant nature, conversion lignin value-added coproducts remains a considerable technical challenge. In this study, we employ base-catalyzed depolymerization (BCD) using process-relevant solid stream produced via deacetylation, mechanical refining, enzymatic hydrolysis enable biological conversion. BCD was conducted with substrate...
Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in nature typically attributed to fungi, growing evidence suggests that bacteria also catabolize this complex biopolymer. However, the spatiotemporal mechanisms for catabolism remain unclear. Improved understanding biological process would aid our collective knowledge both carbon cycling microbial strategies valorize value-added compounds. Here, we examine modifications exoproteome three...
Abstract Background Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for development of cost-effective processes ethanol production. Irpex lacteus an efficient microorganism wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected investigate enzymatic system involved in lignocellulose decay, and its secretome compared those from Phanerochaete chrysosporium Pleurotus ostreatus...
Bio-manufacturing of high-value chemicals in parallel to renewable biofuels has the potential dramatically improve overall economic landscape integrated lignocellulosic biorefineries. However, this will require generation carbohydrate streams from lignocellulose a form suitable for efficient microbial conversion and downstream processing appropriate desired end use, making process development, along with selection target molecules, crucial biorefinery. Succinic acid (SA), molecule, can be...
Fungal ligninolytic enzymes are able to depolymerize solid lignin and the presence of an aromatic catabolic bacterium enhances this effect.
KT2440 is a promising bacterial chassis for the conversion of lignin-derived aromatic compound mixtures to biofuels and bioproducts. Despite inherent robustness this strain, further improvements catabolism toxicity tolerance
Valorization of all major lignocellulose components, including lignin, cellulose, and hemicellulose is critical for an economically viable bioeconomy. In most biochemical conversion approaches, the standard process separately upgrades sugar hydrolysates lignin. Here, we present a new concept based on engineered microbe that could enable simultaneous upgrading streams, which has ultimate potential to reduce capital cost metabolic engineering strategies. Pseudomonas putida robust microorganism...
Abstract Expanding the portfolio of products that can be made from lignin will critical to enabling a viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield production tricarboxylic acid cycle-derived building block chemical, itaconic acid, model aromatic compounds and aromatics derived lignin. We develop nitrogen starvation-detecting biosensor dynamic two-stage bioproduction in which is produced during non-growth associated phase. Through use two distinct pathways,...