A5032164107- Environmental Toxicology and Ecotoxicology
- Aquaculture disease management and microbiota
- Pharmaceutical and Antibiotic Environmental Impacts
- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Toxic Organic Pollutants Impact
- Pesticide and Herbicide Environmental Studies
- Effects and risks of endocrine disrupting chemicals
- Microplastics and Plastic Pollution
- Bacteriophages and microbial interactions
- Fish Ecology and Management Studies
- Animal Genetics and Reproduction
- Microbial Community Ecology and Physiology
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Identification and Quantification in Food
- Mercury impact and mitigation studies
- Antimicrobial Peptides and Activities
- Microbial infections and disease research
- Aquatic life and conservation
- CRISPR and Genetic Engineering
- Heavy Metal Exposure and Toxicity
- Polyomavirus and related diseases
- Microbial bioremediation and biosurfactants
University of Waterloo
2005-2025
Protocols for evaluating chemical toxicity at the cellular level using fish cell lines are described in this unit. Routine methodologies growing salmonid lines, and them aquatic toxicology studies that support mandate of Organization Economic Co-operation Development (OECD) to reduce use whole animals testing, presented. Rapid, simple, cost-effective tests viability cells with three indicator dyes per sample provides a broad overview sensitivity contaminants. This fluorometric assay...
This unit describes protocols for growing salmonid cell lines and using them in vitro toxicology studies. Cell viability of cultures is assessed with three indicator dyes: alamar blue metabolic activity, CFDA-AM membrane integrity, neutral red lysosomal activity. These are essential tools investigating environmental toxicity at the cellular level.
The ability of a ciliate to inactivate bacteriophage was studied because these viruses are known influence the size and diversity bacterial populations, which affect nutrient cycling in natural waters effluent quality sewage treatment, ciliates ubiquitous aquatic environments, including treatment plants. Tetrahymena thermophila used as representative ciliate; T4 model bacteriophage. titer monitored on Escherichia coli B double-agar overlay assay. were incubated together under different...
Nine samples of whole effluent from the operation an industrial plant over course one year, were tested on rainbow trout for lethality and gill cell line, RTgill-W1, metabolic activity, plasma membrane integrity, lysosomal as measured by using alamar Blue (AB), 5-carboxyfluorescein diacetate acetoxymethyl (CFDA-AM), neutral red (NR) assays, respectively. None nine caused a loss only two transitory decline in metabolism. Three massive vacuolisation RTgill-W1 cells, which was accompanied...
Abstract Bacterivory by ciliates in various water ecosystems, both natural and artificial, plays a significant role on the microbial population composition consequently affects quality. A convenient, rapid inexpensive methodology to evaluate capacity of ciliate protozoan Tetrahymena thermophila for bacterivory was developed utilizing fluorescent protein expressing bacteria (FPEB) microtitre plate fluorimeter. correlated with loss fluorescence measured fluorimeter confirmed microscopy showing...
Abstract Because the range of biological mechanisms responsible for inactivation viruses in man-made and natural water systems is poorly understood, involvement free-living ciliated protozoan, Tetrahymena thermophila, viral was investigated. The ciliate found to remove bacteriophage MS2 when phage were co-incubated a simple salt solution. enumerated as plaque forming units (pfus). removal achieved only by living not formalin-fixed ciliates, blocked treatments that impaired formation food...
Monolayer cultures of the rainbow trout cell line, RTgutGC, were used to study actions on intestinal epithelium a potential feed additive, flavanone naringenin. Although having little effect viability or morphology, naringenin altered F-actin staining pattern. Stress fibers disappeared and peripheral actin became more intense. Naringenin enhanced barrier function, as measured by transepithelial electrical resistance (TEER) Lucifer yellow permeability. When cell-free gaps wounds created,...