A5028847166- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
- Disaster Response and Management
- Hepatitis B Virus Studies
- Virus-based gene therapy research
Friedrich-Loeffler-Institut
2018-2024
The 2014–2016 Ebola virus (EBOV) outbreak in West Africa highlighted the need for improved therapeutic options against this virus. Approaches targeting host factors/pathways essential are advantageous because they can potentially target a wide range of viruses, including newly emerging ones and development resistance is less likely than when directly. However, systematic approaches screening factors important EBOV have been hampered by necessity to work with at biosafety level 4 (BSL4). In...
The arenavirus nucleoprotein (NP) plays an important role in the virus’ ability to block interferon (IFN) production, and its exonuclease function appears contribute this activity. However, efforts analyze contribution are complicated by functional overlap between active site a neighboring region involved IKKε-binding subsequent inhibition of IRF3 activation, which also IFN production. To circumvent issue, we mutated residue located away from that is binding dsRNA substrate being targeted...
The highly pathogenic arenavirus, Junín virus (JUNV), expresses three truncated alternative isoforms of its nucleoprotein (NP), i.e., NP
Tacaribe virus (TCRV) is the prototype of New World arenaviruses (also known as TCRV serocomplex viruses). While not itself a human pathogen, many closely related members this group cause hemorrhagic fever, and thus has long served an important BSL2 system for research into diverse areas arenavirus biology. Due to its widespread use, coding-complete sequence both S L segments bipartite genome been publically available almost 30 years. However, more recently, found contain significant...
New World arenaviruses are rodent-transmitted viruses and include a number of pathogens that responsible for causing severe human disease. This includes Junín virus (JUNV), which is the causative agent Argentine hemorrhagic fever. The wild nature mobility rodent reservoir host makes it difficult to control disease, currently passive immunization with high-titer neutralizing antibody-containing plasma from convalescent patients only specific therapy. However, dwindling supplies naturally...
Replication-competent reporter-expressing viruses are crucial tools in molecular virology with applications that range from antiviral screening to live-cell imaging of protein spatiotemporal dynamics. However, there is currently little information available regarding viable strategies develop arenaviruses. To address this, we used Tacaribe virus (TCRV), an apathogenic BSL2 arenavirus, assess the feasibility different reporter expression approaches. We first generated trisegmented TCRV either...