A5058238720- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Lanthanide and Transition Metal Complexes
- Lipid Membrane Structure and Behavior
- Electrochemical Analysis and Applications
- Advanced MRI Techniques and Applications
- Trace Elements in Health
- MRI in cancer diagnosis
- Analytical Chemistry and Sensors
- DNA and Nucleic Acid Chemistry
- Autophagy in Disease and Therapy
- Electrochemical sensors and biosensors
- Luminescence and Fluorescent Materials
- Cellular transport and secretion
- Magnetism in coordination complexes
- Chemical Synthesis and Analysis
- bioluminescence and chemiluminescence research
- Receptor Mechanisms and Signaling
- Click Chemistry and Applications
- Adenosine and Purinergic Signaling
- RNA Interference and Gene Delivery
- Enzyme Structure and Function
- Light effects on plants
- Magnesium in Health and Disease
- Biotin and Related Studies
Tata Institute of Fundamental Research
2014-2025
University of Mumbai
2018
University of California, Berkeley
2007-2011
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2008
Lawrence Berkeley National Laboratory
2007-2008
Princeton University
2007
High relaxivity macromolecular contrast agents based on the conjugation of gadolinium chelates to interior and exterior surfaces MS2 viral capsids are assessed. The proton nuclear magnetic relaxation dispersion (NMRD) profiles conjugates show up a 5-fold increase in relaxivity, leading peak (per Gd3+ ion) 41.6 mM-1 s-1 at 30 MHz for internally modified capsids. Modification was achieved through flexible lysines, while internal modification accomplished by relatively rigid tyrosines. Higher...
MRI contrast agents providing very high relaxivity values can be obtained through the attachment of multiple gadolinium(III) complexes to interior surfaces genome-free viral capsids. In previous studies, enhancement was predicted depend on rigidity linker attaching protein surface. To test this hypothesis, a new set Gd-hydroxypyridonate based prepared and attached genetically introduced cysteine residues flexible rigid linkers. Greater enhancements were seen for that via linkers, validating...
Two high-relaxivity nanoscale magnetic resonance contrast agents have been built using bacteriophage MS2 as a biomolecular scaffold. Protein capsid shells were functionalized on either the exterior or interior surface to display multiple copies of an aldehyde functional group. Subsequently, ∼90 heteropodal bis(hydroxypyridonate)terephthalamide ligands attached these sites through oxime condensation reactions. Upon complexation with Gd3+, ionic relaxivities up 41.6 mM-1 s-1 (30 MHz, 25 °C)...
We report highly selective, novel Cu<sup>2+</sup>chelators that relieve copper induced oxidative stress<italic>in vivo</italic>indicating applicability toward assuaging copper-overload disorders.
We report the serendipitous discovery of an optical mercury sensor while trying to develop a water-soluble manganese probe. The is based on pentaaza macrocycle conjugated hemicyanine dye. earlier designed in our group was used photoinduced electron transfer (PET)-based "turn-on" fluorescent sensors for manganese.(1) In attempt increase water-solubility we changed dye from BODIPY hemicyanine. resultant molecule qHCM afforded distinct reversible change absorption features and concomitant...
A water-soluble, cell-permeable, turn-on Mn 2+ sensor visualizes dynamics live and provides an estimate for endogenous labile in a living mammalian cell.
We report novel ‘turn-on’ fluorescence probes for imaging Mn<sup>2+</sup> in live cells.
Hydrogen peroxide (H2O2) plays a critical role in the regulation and progress of autophagy, an essential recycling process that influences cellular homeostasis stress response. Autophagy is characterized by formation intracellular vesicles analogous to recycle "bags" called autophagosomes, which fuse with lysosomes form autolysosomes, eventually ending up as lysosomes. We have developed two novel autophagic vesicle-targeted peptide-based sensors, ROSA for H2O2 pHA pH, simultaneously track pH...
Raman scattering comes with the promise of multiplexed imaging bio-analytes within living systems because narrow peak-widths. This feature combined strategy utilizing alkyne/nitrile tags which have peaks in cell-silent region, a window, where inherent biomolecules no features, has catalyzed development probes. In this context, Raman-responsive ratiometric sensors will be key players affording and quantification cells. A major challenge however is low cross-sections alkynes/nitriles, leading...
Abstract Manganese (Mn) is essential for neuronal function, yet toxic at high concentrations. Environmental and occupational exposure to concentrations of Mn causes manganism, a well-defined movement disorder in humans, with symptoms resembling Parkinson’s disease (PD). However, manganism distinct from PD the neural basis its pathology poorly understood. To address this issue, we generated zebrafish model by incubating larvae rearing medium containing Mn. We find that Mn-treated exhibit...
Elucidating dynamics in transition-metal distribution and localization under physiological pathophysiological conditions is central to our understanding of metal-ion regulation. In this Forum Article, we focus on manganese specifically recent developments that point the relevance Golgi apparatus detoxification when essential metal ion overaccumulated because either environmental exposure or mutations efflux transporters. order further evaluate role as a manganese-ion storage compartment...
Phosphoinositides, phospholipids that are key cell-signal mediators, present at very low levels in cellular membranes and within nuclei. Phosphatidylinositol-(3,4,5)-trisphosphate (PIP3), a phosphoinositide barely resting cell membranes, is produced when cells receive either growth, proliferation, or movement signals. Aberrant PIP3 associated with the formation of cancers. pools also nucleus, specifically nucleolus. However, questions related to organization function this lipid such...
A novel activity-based sensor afforded 63 times fluorescence enhancement in the presence of Cu 2+ ions. The functioned only glutathione and ambient oxygen via a plausible II hydroperoxo species.
Abstract Um die klinischen Anwendungsmöglichkeiten der Kernspintomographie (MRI) zu verbessern, wird intensiv nach effizienteren Kontrastmitteln gesucht. Dazu müssen Koordinationschemie von Gadolinium(III) und Parameter, seine Wirksamkeit als Protonenrelaxationsagens beeinflussen, berücksichtigt werden. Will man jeden Parameter optimieren, so sind auch Eigenschaften wie Löslichkeit In‐vivo‐Toxizität beachten, was Entwicklung sicherer starker Relaxationsagentien einem anspruchsvollen Ziel...
Metal-ion-mediated interactions between calcium-binding peripheral proteins and membrane phospholipids is a key feature of multiple cell signaling processes. The molecular basis for the interaction involves displacement inner-sphere water molecules on calcium ions by phosphate groups phospholipids. On this fundamental mechanism, we have devised novel "turn-on" optical sensing strategy anionic using lanthanide reconstituted protein. "lanthano" protein turns selectively in presence crucial...
Phosphoinositides are critical cell-signal mediators present on the plasma membrane. The dynamic change of phosphoinositide concentrations membrane including clustering and declustering mediates signal transduction. importance phosphoinositides is scored by fact that they participate in almost all cell-signaling events, a defect metabolism linked to multiple diseases cancer, bipolar disorder, type-2 diabetes. Optical sensors for visualizing distribution can provide information dynamics. This...
Autophagy is an essential cellular degradation process. Impaired autophagy has been linked to multiple disorders, including cancer and neurodegeneration. Tracking the autophagic flux in living cells will provide mechanistic insights into allow rapid screening of modulators as potential therapeutics. Imaging track demands a cell-permeable probe that can specifically target vesicles report on extent autophagy. Existing fluorescent protein-based probes for imaging but are cell-impermeable...
Anionic phospholipids are key cell signal mediators. The distribution of these lipids on the membrane and intracellular organelle membranes guides recruitment signaling proteins leading to regulation cellular processes. Hence, fluorescent sensors that can detect anionic within living cells provide a handle into revealing molecular mechanisms underlying lipid-mediated regulation. A major challenge in detection is related presence mostly inner leaflet plasma organelles. cell-permeable would an...
Abstract Anionic phospholipids are essential structural components of cell membranes. Spatiotemporal dynamics these lipids play central roles in regulating signalling events, membrane trafficking, maintenance cell‐shape, and cargo transport. On the other hand, defects anionic phospholipid metabolism linked to multiple diseases. Hence, ability visualize their living cells can afford mechanistic insights into vital processes, guide development therapeutics, lead diagnostic agents. In this...
Tolga Karsili opened the discussion of paper by Dimitra Markovitsi: It is well known that acidic substituents in aromatic systems contain πσ* states are dissociative with respect to X–H bond fission (where X = N, O or S). also photochemistry simple