A5013565576- Ion Channels and Receptors
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Ion Transport and Channel Regulation
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Amyotrophic Lateral Sclerosis Research
- Cholinesterase and Neurodegenerative Diseases
- Biochemical Analysis and Sensing Techniques
- Pain Mechanisms and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Phagocytosis and Immune Regulation
- Pulmonary Hypertension Research and Treatments
- Single-cell and spatial transcriptomics
- Nerve injury and regeneration
- Neurogenetic and Muscular Disorders Research
- Coordination Chemistry and Organometallics
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
Czech Academy of Sciences, Institute of Experimental Medicine
2018-2024
Charles University
2020-2024
Czech Academy of Sciences
2018-2023
Abstract Glial cells expressing neuron‐glial antigen 2 (NG2), also known as oligodendrocyte progenitor (OPCs), play a critical role in maintaining brain health. However, their ability to differentiate after ischemic injury is poorly understood. The aim of this study was investigate the properties and functions NG2 glia brain. Using transgenic mice, we selectively labeled NG2‐expressing progeny both healthy focal cerebral ischemia (FCI). single‐cell RNA sequencing, classified glial into five...
Astrocytic Aquaporin 4 (AQP4) and Transient receptor potential vanilloid (TRPV4) channels form a functional complex that likely influences cell volume regulation, the development of brain edema, severity ischemic injury. However, it remains to be fully elucidated whether blocking these can serve as therapeutic approach alleviate consequences having stroke.In this study, we used in vivo magnetic resonance imaging (MRI) quantify extent lesions one day (D1) seven days (D7) after permanent...
N-methyl-D-aspartate receptors (NMDARs) play a significant role in developing several central nervous system (CNS) disorders. Currently, memantine, used for treating Alzheimer's disease, and ketamine, known its anesthetic antidepressant properties, are two clinically NMDAR open-channel blockers. However, despite extensive research into modulators, many have shown either harmful side effects or inadequate effectiveness. For instance, dizocilpine (MK-801) is recognized powerful psychomimetic...
The proper function of the nervous system is dependent on balance ions and water between intracellular extracellular space (ECS). It has been suggested that interaction aquaporin-4 (AQP4) transient receptor potential vaniloid isoform 4 (TRPV4) channels play a role in cell volume regulation, indirectly, ECS volume. Using real-time iontophoretic method, we studied changes diffusion parameters: fraction α (α = fraction/total tissue volume) tortuosity λ (λ2 free/apparent coefficient) mice with...
Introduction Astrocytic Transient receptor potential vanilloid 4 (TRPV4) channels, together with Aquaporin (AQP4), are suspected to be the key players in cellular volume regulation, and therefore may affect development severity of cerebral edema during ischemia. In this study, we examined astrocytic swelling/volume recovery mice TRPV4 and/or AQP4 deletion response vitro ischemic conditions, determine how these channels can edema. Methods We used three models ischemia-related pathological...
In this study, we aimed to disclose the impact of amyloid-β toxicity and tau pathology on astrocyte swelling, their volume recovery extracellular space (ECS) diffusion parameters, namely fraction (α) tortuosity (λ), in a triple transgenic mouse model Alzheimer's disease (3xTg-AD). Astrocyte changes, which reflect ability take up ions/neurotransmitters, were quantified during after exposure hypo-osmotic stress, or hyperkalemia acute hippocampal slices, correlated with alterations ECS...
Abstract N -methyl-D-aspartate receptors (NMDARs) play a significant role in developing several central nervous system (CNS) disorders. Currently, memantine, used for treating Alzheimer’s disease, and ketamine, known its anesthetic antidepressant properties, are two clinically NMDAR open-channel blockers. However, despite extensive research into modulators, many have shown either harmful side effects or inadequate effectiveness. For instance, dizocilpine (MK-801) is recognized powerful...
ABSTRACT Glia cells expressing neuron-glial antigen 2 (NG2) play a critical role as oligodendrocyte precursor (OPCs) in the healthy brain; however, their differentiation potential after ischemic injury remains an unresolved question. Here, we aimed to elucidate heterogeneity and of NG2 glia brain. We used transgenic mice label NG2-expressing progeny with red fluorescent protein tdTomato brains those focal cerebral ischemia (FCI). Based on single-cell RNA sequencing, labeled glial were...
Abstract Proteins from the Bcl-2 family play an essential role in regulation of apoptosis. However, they also possess cell death-unrelated activities that are less well understood. This prompted us to study apoptosis-unrelated Bax and Bak, pro-apoptotic members family. We prepared Bax/Bak-deficient human cancer cells different origin found while respiration glioblastoma U87 was greatly enhanced, B lymphoma HBL-2 slightly suppressed. proliferated faster culture, formed tumours more rapidly...
Objectives: Development of pulmonary arterial hypertension (PAH) in systemic sclerosis (SSc) represents one the most serious complications. The study aimed at assessing right ventricular (RV) global longitudinal strain and its relation to artery pressure. Methods: Echocardiography was performed 74 patients whom 60 had SSc 14 mixed connective tissue disease (MCTD). Besides routine left ventricle assessment pressure estimation by Doppler echocardiography, RV fractional area change (FAC) 2D...