A5058786627- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Silk-based biomaterials and applications
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Neurological Disease Mechanisms and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Extracellular vesicles in disease
- Complement system in diseases
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Biochemical Analysis and Sensing Techniques
- Tissue Engineering and Regenerative Medicine
- Diabetes Management and Research
- Cytomegalovirus and herpesvirus research
- Alzheimer's disease research and treatments
- Bone Tissue Engineering Materials
- RNA Research and Splicing
- Barrier Structure and Function Studies
Czech Academy of Sciences, Institute of Biotechnology
2018-2024
Czech Academy of Sciences
2018-2024
University of Chemistry and Technology, Prague
2021-2024
Charles University
2019-2021
Ischemic stroke is a well-recognized disease of aging, yet it unclear how the age-dependent vulnerability occurs and what are underlying mechanisms. To address these issues, we perform comprehensive RNA-seq analysis ischemic stroke, their interaction in 3- 18-month-old mice. We assess differential gene expression across injury status age, estimate cell type proportion changes, assay results against range transcriptional signatures from literature, unsupervised co-expression analysis,...
Despite advances in acute care, ischemic stroke remains a major cause of long-term disability. Approaches targeting both neuronal and glial responses are needed to enhance recovery improve outcome. The complement C3a receptor (C3aR) is regulator inflammation with roles neurodevelopment, neural plasticity, neurodegeneration. Using mice lacking C3aR (C3aR-/-) overexpressing the brain, we uncovered 2 opposing effects signaling on functional after stroke: inhibition phase facilitation later...
The role of nonneuronal cells in the resolution cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial single-cell transcriptomic profiling male mouse brain during first week injury. Cortical gene expression was severely disrupted, defined by inflammation cell death lesion core, glial scar formation orchestrated multiple types on periphery. identified as a zone with intense cell–cell communication,...
A bstract NG2 glia display wide proliferation and differentiation potential under physiological pathological conditions. Here, we examined these two features following different types of brain disorders such as focal cerebral ischemia (FCI), cortical stab wound (SW), demyelination (DEMY) in 3‐month‐old mice, which are labeled by tdTomato the Cspg4 promoter. To compare expression profiles CNS injuries, employed single‐cell RT‐qPCR self‐organizing Kohonen map analysis tdTomato‐positive cells...
Abstract Background Glucose homeostasis is dependent on functional pancreatic α and ß cells. The mechanisms underlying the generation maturation of these endocrine cells remain unclear. Results We unravel molecular mode action ISL1 in controlling cell fate formation pancreas. By combining transgenic mouse models, transcriptomic epigenomic profiling, we uncover that elimination Isl1 results a diabetic phenotype with complete loss cells, disrupted islet architecture, downregulation key ß-cell...
Abstract BACKGROUND Recent advances allowing quantification of RNA from single cells are revolutionizing biology and medicine. Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). However, RT is recognized as a known source variability, particularly with low amounts RNA. Recently, several new transcriptases (RTases) the potential to decrease loss information have been developed, but knowledge their performance limited. METHODS We compared 11 RTases...
Abstract NEUROD1 is a transcription factor that helps maintain mature phenotype of pancreatic β cells. Disruption Neurod1 during development causes severe neonatal diabetes; however, the exact role in differentiation programs endocrine cells unknown. Here, we report crucial regulatory network lineage commitment and differentiation. Mechanistically, transcriptome chromatin landscape analyses demonstrate inactivation triggers downregulation factors upregulation non-endocrine genes within...
In this study, we aimed to disclose the impact of amyloid-β toxicity and tau pathology on astrocyte swelling, their volume recovery extracellular space (ECS) diffusion parameters, namely fraction (α) tortuosity (λ), in a triple transgenic mouse model Alzheimer's disease (3xTg-AD). Astrocyte changes, which reflect ability take up ions/neurotransmitters, were quantified during after exposure hypo-osmotic stress, or hyperkalemia acute hippocampal slices, correlated with alterations ECS...
Abstract Embryos are regeneration and wound healing masters. They not only rapidly close their wounds, remodel injured tissue without a scar, but also regenerate body parts. Many animal models with variable regenerative capabilities have already been studied. Additionally, the introduction of high throughput techniques, novel mechanisms including genes signaling pathways, specialized cell types required for control in spatial temporal aspects identified. Until now our knowledge has limited...
Abstract The role of non-neuronal cells in the resolution cerebral ischemia remains to be fully understood. To decode key cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling mouse brain tissue during first week injury. Cortical gene expression was severely disrupted, being defined by inflammation cell death lesion core, glial scar formation on periphery. For each three major populations, an inflammatory-responsive state, resembling...
ABSTRACT Background Recent technical advances allowing quantification of RNA from single cells are revolutionizing biology and medicine. Currently, almost all single-cell transcriptomic protocols rely on conversion to cDNA by reverse transcription (RT). However, RT is recognized as highly limiting step due its inherent variability suboptimal sensitivity, especially at minute amounts RNA. Primary factor influencing outcome transcriptase (RTase). Recently, several new RTases with potential...
Abstract Ischemic stroke is one of the leading causes mortality and major healthcare economic burden. It a well-recognized disease aging, yet it unclear how age-dependent vulnerability occurs what are underlying mechanisms. To address these issues, we performed comprehensive RNA-Seq analysis ischemic their interaction using model permanent middle cerebral artery occlusion (MCAO) in 3 18 month old female mice. We assessed differential gene expression across injury status age, estimated cell...