A5085972469- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Neurological Disease Mechanisms and Treatments
- Hedgehog Signaling Pathway Studies
- Nuclear Receptors and Signaling
- MicroRNA in disease regulation
- Anesthesia and Neurotoxicity Research
- Pharmacological Effects and Assays
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
Immunai (United States)
2024
Czech Academy of Sciences, Institute of Experimental Medicine
2016-2021
Czech Academy of Sciences
2015-2018
Charles University
2016-2018
Ischemic stroke is a well-recognized disease of aging, yet it unclear how the age-dependent vulnerability occurs and what are underlying mechanisms. To address these issues, we perform comprehensive RNA-seq analysis ischemic stroke, their interaction in 3- 18-month-old mice. We assess differential gene expression across injury status age, estimate cell type proportion changes, assay results against range transcriptional signatures from literature, unsupervised co-expression analysis,...
The tamoxifen-inducible Cre-loxP system is widely used to overcome gene targeting pre-adult lethality, modify a specific cell population at desired time-points, and visualize trace cells in fate-mapping studies. In this study we focused on tamoxifen degradation kinetics, because for all genetic studies, the period during which or its metabolites remain active CNS, essential. Additionally, aimed define administration scheme, enabling maximal recombination rate together with minimal animal...
A bstract NG2 glia display wide proliferation and differentiation potential under physiological pathological conditions. Here, we examined these two features following different types of brain disorders such as focal cerebral ischemia (FCI), cortical stab wound (SW), demyelination (DEMY) in 3‐month‐old mice, which are labeled by tdTomato the Cspg4 promoter. To compare expression profiles CNS injuries, employed single‐cell RT‐qPCR self‐organizing Kohonen map analysis tdTomato‐positive cells...
NG2 cells, a fourth glial cell type in the adult mammalian central nervous system, produce oligodendrocytes healthy tissue, and display wide differentiation potential under pathological conditions, where they could give rise to reactive astrocytes. The factors that control of cells after focal cerebral ischemia (FCI) are largely unknown. Here, we used transgenic Cspg4‐cre/Esr1/ROSA26Sortm14(CAG‐tdTomato) mice, which tamoxifen administration triggers expression red fluorescent protein...
Abstract NG2 cells represent precursors of oligodendrocytes under physiological conditions; however, following cerebral ischemia they play an important role in glial scar formation. Here, we compared the expression profiles oligodendroglial lineage cells, after focal (FCI) and Alzheimer's‐like pathology using transgenic mice, which enables genetic fate‐mapping Cspg4‐positive their progeny, based on red fluorescent protein tdTomato. tdTomato‐positive possessed profile oligodendrocytes; cell...
Abstract Background Advances in single-cell technologies enable the unbiased study of cellular heterogeneity. Recently, RNA sequencing (scRNA-seq) has been utilised on intestinal and blood samples from patients with inflammatory bowel disease (IBD) healthy individuals, often conjunction cell surface proteome TCR repertoire analyses. These individual studies revealed novel subpopulations immune, mesenchymal, epithelial cells UC CD, but are not sufficiently powered to consistently identify...