A5024440590- Pancreatic function and diabetes
- Diabetes Management and Research
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Hydrogels: synthesis, properties, applications
- Blood properties and coagulation
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Cannabis and Cannabinoid Research
- Epigenetics and DNA Methylation
- Burn Injury Management and Outcomes
- Diabetes and associated disorders
- Genetics, Bioinformatics, and Biomedical Research
- Platelet Disorders and Treatments
- Gene expression and cancer classification
- Tendon Structure and Treatment
- Pressure Ulcer Prevention and Management
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
University of Virginia
2020-2024
Charlottesville Medical Research
2021-2023
North Carolina State University
2018-2021
UNC/NCSU Joint Department of Biomedical Engineering
2021
University of North Carolina at Chapel Hill
2021
Microporous annealed particle (MAP) hydrogel has been a promising scaffold platform technology to promote immediate tissue integration in injured environments. The addition of growth factors the potential accelerate and enhance scaffold-mediated healing. Growth factor releasing scaffolds face translational hurdle limited solubilized protein shelf stability; however, address this we present lyophilized MAP which can be effectively rehydrated directly prior use. Our new approach includes...
Abstract Platelets mediate hemostasis by aggregating and binding to fibrin promote clotting. Over time, platelets contract the network induce clot retraction, which contributes wound healing outcomes increasing stability improving blood flow ischemic tissue. In this study, development of hollow platelet‐like particles (PLPs) that mimic native platelet function retraction in a controlled manner is described it demonstrated retraction‐inducing PLPs vivo. are created coupling fibrin‐binding...
The inflammatory foreign body response (FBR) is the main driver of biomaterial implant failure. Current strategies to mitigate onset a FBR include modification surface, release anti-inflammatory drugs, and cell-scale porosity. microporous annealed particle (MAP) scaffold platform an injectable, porous composed individual microgels, which are in situ provide structurally stable with microporosity. MAP does not induce discernible vivo and, therefore, can be used "blank canvas" for...
The microporous annealed particle (MAP) scaffold platform is a subclass of granular hydrogels. It composed an injectable slurry microgels that can form structurally stable with cell-scale porosity in situ following secondary light-based chemical crosslinking step (i.e., annealing). MAP has shown success variety regenerative medicine applications, including dermal wound healing, vocal fold augmentation, and stem cell delivery. This paper describes the methods for synthesis characterization...
Type 1 diabetes (T1D) is caused by the autoimmune loss of insulin-producing beta cells in pancreas. The only clinical approach to patient management blood glucose that doesn't require exogenous insulin pancreas or islet transplantation. Unfortunately, donor islets are scarce and there substantial immediately after transplantation due, part, local inflammatory response. delivery stem cell-derived (
Abstract Sample multiplexing in flow cytometry is a powerful technique which allows for reduction of error, inclusion control samples batch effect correction, and both time consumable usage. Current industry standard barcoding mass an intracellular reagent, requires fixation permeabilization sample prior to barcoding. We developed barcode using the ubiquitous well-tolerated membrane labeling lectin, wheat germ agglutinin. This effectively labels all tested cell types, live fixed. determine...
The microporous annealed particle (MAP) scaffold platform is a subclass of granular hydrogels. It composed an injectable slurry microgels that can form structurally stable with cell-scale porosity in situ following secondary light-based chemical crosslinking step (i.e., annealing). MAP has shown success variety regenerative medicine applications, including dermal wound healing, vocal fold augmentation, and stem cell delivery. This paper describes the methods for synthesis characterization...
The aim was to investigate if MAP gel can support the function of whole islets or dissociated islet cells, and improve transplantation under kidney capsule within epididymal fat pad. A) Whole BL/6 were transplanted into syngeneic diabetic recipients in presence absence gel. 100 (n=6/group). Mice receiving transplant pad divided 6 groups (n=2/group): 300 islets; 400 alone; 200, with Blood glucose (BG) measured daily. B) NP NOD mice (n=4/group) analyze immune response. Graft-containing excised...
Abstract The wound healing response is critical to maintain tissue function after injury. Inflammation needed sterilize the site, but this results in scar formation and impaired function. Topical anti-microbial use, fetal healing, limited studies germ-free animals suggest that degree of microbial contamination at a site positively correlates with recovery time. However, tools identify molecular signals cell types alter repair verses have been limited. Here we test hypothesis...
Introduction: To investigate if coating dissociated or whole islets with microporous annealed particle (MAP) gel could support cell function and viability improve islet transplantation outcomes. Methods: a) C57BL/6 mouse were mixed MAP cultured in 24 well plates at 37°C. The of was scored after Propidium Iodide-Fluorescein Diacetate (PI/FDA) staining Glucose stimulated insulin secretion (GSIS) assay performed on Day 1, 3, 5, 7 post-culture. experiment done thrice. b) Single cells from gel,...