A5005866110- Renal and related cancers
- Renal cell carcinoma treatment
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Organ Donation and Transplantation
- Microwave Imaging and Scattering Analysis
- Genetic and Kidney Cyst Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Geophysical Methods and Applications
- Cardiovascular Disease and Adiposity
- Ocular Diseases and Behçet’s Syndrome
- Muscle metabolism and nutrition
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- BRCA gene mutations in cancer
- Tissue Engineering and Regenerative Medicine
- Bone health and treatments
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
New York Eye and Ear Infirmary
2006-2024
Tufts University
2018-2024
MaineHealth
2023-2024
Icahn School of Medicine at Mount Sinai
2024
Maine Medical Center Research Institute
2011-2023
University of Maine
2018-2023
Brigham Young University
2019-2023
Maine Medical Center
2009-2022
X-Fab (Germany)
2017
Anna Needs Neuroblastoma Answers
2017
Abstract Abs of the IgG isotype are efficiently transported from mother to neonate and have an extended serum t1/2 compared with other isotypes. Circumstantial evidence suggests that MHC class I-related protein, neonatal FcR (FcRn), is responsible for both in vivo functions. To understand phenotypes imposed by FcRn, we produced analyzed mice a defective FcRn gene. The results provide direct perinatal transport protection catabolism mediated latter function key homeostasis, essential...
Reactive oxygen species, especially hydrogen peroxide, are important in cellular signal transduction. However, excessive amounts of these species damage tissues and cells by oxidizing virtually all biomolecules. Peroxiredoxin 6 (PRDX6) (also called antioxidant protein 2, or AOP2) is a novel peroxiredoxin family member whose function vivo unknown. Through immunohistochemistry, we have determined that the PRDX6 was widely expressed every tissue examined, most abundantly epithelial cells. It...
Journal Article Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application humorally mediated autoimmune disease Get access Stefka B. Petkova, Petkova 1Jackson Laboratory, Bar Harbor, ME 04609, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Shreeram Akilesh, Akilesh USA4Present address: Department Pathology, Washington University, St Louis, MO, Thomas J. Sproule, Sproule Gregory Christianson,...
Embryonic nephron progenitor cells are segregated in molecularly distinct compartments of unknown function. Our study reveals an integral role for bone morphogenetic protein-SMAD promoting transition progenitors from the primitive Cbp/p300-interacting transactivator 1 expressing (CITED1+) compartment to uniquely sine oculis-related homeobox 2 (SIX2-only) where they become inducible by wingless-type mouse mammary tumor virus integration site family member (WNT)/β-catenin signaling....
Forkhead transcription factors are essential for diverse processes in early embryonic development and organogenesis. Foxd1 is required during kidney its inactivation results failure of nephron progenitor cell differentiation. expressed interstitial cells adjacent to cells, suggesting an role the niche nephrogenesis. To better understand how cortical general, FOXD1 particular, influence niche, we examined differentiation states two subtypes Foxd1(-/-) tissue. We found that although retained a...
The iterative formation of nephrons during embryonic development relies on continual replenishment progenitor cells throughout nephrogenesis. Defining molecular mechanisms that maintain and regulate this pool is essential to understanding nephrogenesis in developmental regenerative contexts. Maintenance nephron progenitors absolutely dependent BMP7 signaling, Bmp7-null mice exhibit rapid loss progenitors. However, the signal transduction machinery operating downstream as well precise target...
Recent studies indicate that nephron progenitor cells of the embryonic kidney are arranged in a series compartments an increasing state differentiation. The earliest compartment, distinguished by expression CITED1, possesses greater capacity for renewal and differentiation than later compartments. Signaling events governing progression through stages poorly understood, their elucidation will provide key insights into normal dysregulated nephrogenesis, as well regenerative processes follow...
Matrix proteoglycans such as biglycan (Bgn) dominate skeletal tissue and yet its exact role in regulating bone function is still unclear. In this paper we describe the potential of fracture healing process. We hypothesized that Bgn could regulate because previous work showing it can affect normal formation. To test hypothesis, created fractures femurs 6-week-old male wild type (WT or Bgn+/0) Bgn-deficient (Bgn-KO Bgn-/0) mice using a custom-made standardized device, analyzed process over...
Prostate cancer (PC) is a leading cause of death in men however the factors that regulate its progression and eventual metastasis to bone remain unclear. Here we show WISP1/CCN4 expression prostate tissues was up-regulated early stages disease and, further, it correlated with increased circulating levels WISP1 sera patients at disease. also elevated mouse model TRAMP hypoplastic diseased tissue develops prior advanced carcinoma formation. When ability anti-WISP1 antibodies reduce spread...
Molecular- and cellular-based therapies have the potential to reduce obesity-associated disease. In response cold, beige adipocytes form in subcutaneous white adipose tissue convert energy stored metabolic substrates heat, making them an attractive therapeutic target. We developed a robust method generate renewable source of human from induced pluripotent stem cells (iPSCs). Developmentally, these are derived FOXF1+ mesoderm progress through expandable mural-like mesenchymal cell (MSC)...
The canonical Wnt pathway transcriptional co-activator β-catenin regulates self-renewal and differentiation of mammalian nephron progenitor cells (NPCs). We modulated levels in NPC cultures using the GSK3 inhibitor CHIR99021 (CHIR) to examine opposing developmental actions β-catenin. Low CHIR-mediated maintenance expansion NPCs are independent direct engagement TCF/LEF/β-catenin complexes at low CHIR-dependent cell-cycle targets. In contrast, high CHIR, TCF7/LEF1/β-catenin replaced...
Contrary to its classic role in restraining cell proliferation, we demonstrate here a divergent function of p53 the maintenance self-renewal nephron progenitor pool embryonic mouse kidney. Nephron endowment is regulated by availability and differentiation potential. Conditional deletion cells (Six2Cre+;p53fl/fl) induces progressive depletion Cited1+/Six2+ self-renewing progenitors loss cap mesenchyme (CM) integrity. The Six2(p53-null) CM disorganized, with interspersed stromal an absence...
ABSTRACT Periodontal disease is one of the most prevalent chronic inflammatory diseases. There a genetic component to susceptibility and resistance this disease. Using mouse model, we investigated progression alveolar bone loss by gene expression profiling susceptible resistant strains (BALB/cByJ A/J, respectively). We employed novel sensitive quantitative real-time PCR method compare basal RNA transcription 48-gene set in gingiva spleen subsequent changes due Porphyromonas gingivalis oral...
Recent developments in sequence databases provide the opportunity to relate expression pattern of genes their genomic position, thus creating a transcriptome map. Quantitative trait loci (QTL) are phenotypically-defined chromosomal regions that contribute allelically variant biological traits, and by overlaying QTL on transcriptome, search for candidate becomes extremely focused.We used our novel data mining tool, ExQuest, select within known diabesity showing enriched primary affected...
A light-harvesting macromolecular assembly (PT-Ru) consisting of a poly(3-hexylthiophene) (P3HT) scaffold and pendant Ru(II) polypyridyl complexes that exhibits rapid efficient formation long-lived charge-separated state is described here. Photoinduced electron transfer from the polymer backbone to was investigated by femtosecond transient absorption spectroscopy. Photoexcitation at 388 nm results in excitation both complexes, with relative probabilities 60 40%, respectively. The dominant...
The trapping of Poly-ADP-ribose polymerase (PARP) on DNA caused by PARP inhibitors (PARPi) triggers acute replication stress and synthetic lethality (SL) in BRCA2-deficient cells. Hence, damage is accepted as a prerequisite for SL In contrast, here we show that inhibiting ROCK cells independently from stress. Such preceded polyploidy binucleation resulting cytokinesis failure. initial mitosis abnormalities are followed other M phase defects, including anaphase bridges abnormal mitotic...