A5075595611- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
- Skin Protection and Aging
- bioluminescence and chemiluminescence research
- Advanced biosensing and bioanalysis techniques
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Light effects on plants
- Microtubule and mitosis dynamics
- Cancer Mechanisms and Therapy
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Protein Tyrosine Phosphatases
- Photochromic and Fluorescence Chemistry
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- 14-3-3 protein interactions
- Click Chemistry and Applications
- Photodynamic Therapy Research Studies
Kanazawa University
2012-2023
Life University
2009
University of California, San Francisco
2008
Tokyo Institute of Technology
2004
The University of Tokyo
2004
Chuo University
2004
Saitama Institute of Technology
2004
University of North Carolina at Chapel Hill
1995-1996
Indiana University School of Medicine
1995-1996
St Thomas' Hospital
1993
Xeroderma pigmentosum is a hereditary disease caused by defective DNA repair. Somatic cell genetics and biochemical studies with cell-free extracts indicate that at least 16 polypeptides are required to carry out the repair reaction proper, i.e. removal of lesion from dual incisions damaged strand. To find if these proteins necessary sufficient for excision repair, they were obtained high level purity in five fractions. The mixture fractions reconstituted nuclease (excinuclease) activity....
Abstract Six new monoclonal antibodies (TDM‐2, TDM‐3, 64M‐2, 64M‐3, 64M‐4 and 64M‐5) specific for ultraviolet (UV) induced DNA damage have been established. In the antibody characterization experiments, two TDM were found to show a dose‐dependent binding UV‐irradiated (UV‐DNA), decrease of UV‐DNA after cyclobutane pyrimidine dimer photo‐reactivation, containing thymine dimers, unchanged photoisomerization (6‐4)photoproducts Dewar photoproducts. These results indicated that epitope was in...
Abstract —We investigated the wavelength dependence of cyclobutane thymine dimer and (6‐4)photo‐product induction by monochromatic UV in region extending from 150 to 365 nm, using an enzyme‐linked immunosorbent assay with two monoclonal antibodies. Calf thymus DNA solution was irradiated with254–365 nm a spectrograph, or with220–300 synchrotron radiation. Thymine dimers (6‐4)photoproducts were fluence‐dependently induced every tested so far. We also examined both types damage below 220 under...
Damaged DNA-binding protein, DDB, is a heterodimer of p127 and p48 with high specificity for binding to several types DNA damage. Mutations in the <i>p48</i> gene that cause loss DDB activity were found subset xeroderma pigmentosum complementation group E (XP-E) patients have linked deficiency global genomic repair cyclobutane pyrimidine dimers (CPDs) these cells. Here we show highly defined system purified factors, can greatly stimulate excision reaction reconstituted XPA, RPA, XPC·HR23B,...
Human DNA repair excision nuclease removes damage by incising on both sides of the lesion in a precise manner. The activity requires participation 16-17 polypeptides. Of these, XPF•ERCC1 complex and XPG were predicted to carry active sites based studies with recombinant proteins yeast homologs these proteins. Furthermore, recent work model (undamaged) substrates have led predictions roles 5′ or 3′ lesion. We used damaged antibodies ERCC1 test predictions. Our results reveal that anti-XPG...
Abstract A toxic endocrine disruptor, 4-n-nonylphenol, was degraded using a well-known TiO2 and visible-light-driven BiVO4 photocatalyst under irradiation from solar simulator. The degradation rates for both are comparable in air-saturated solution though surface area of is much smaller than that TiO2. This fact indicates has great ability to degrade alkylphenols wastewater light.
Human histone H2AX is rapidly phosphorylated on serine 139 in response to DNA double-strand breaks and plays a crucial role tethering the factors involved repair damage signaling. Replication stress caused by hydroxyurea or UV also initiates phosphorylation S-phase cells, although UV-induced non-cycling cells has recently been observed. Here we study human primary fibroblasts under growth-arrested conditions. This reaction absolutely depends nucleotide excision (NER) mechanistically distinct...
A complex, which consists of ERCC1 (38 kDa) and a 112-kDa protein, was purified from HeLa cells to homogeneity. This complex complemented the nucleotide excision repair defects rodent ERCC-1, ERCC-4, human XP-F mutant cell-free extracts, indicating that protein is XPF/ERCC4 providing direct biochemical evidence XPF ERCC4 are identical. The XPF/ERCC4-ERCC1 has an endonuclease activity with preference for single-stranded DNA region duplex "bubble" structure. also nicks supercoiled weakly, this...
Summary We have isolated and characterized a new ultraviolet B (UV‐B)‐resistant mutant, uvi4 ( UV‐B‐insensitive 4 ), of Arabidopsis. The fresh weight (FW) plants grown under supplemental UV‐B light was more than twice that the wild‐type. No significant difference found in their ability to repair UV‐B‐induced cyclobutane pyrimidine dimers, or amount absorptive compounds, both which are well‐known factors contribute UV sensitivity. Positional cloning revealed UVI4 gene encodes novel basic...
The xeroderma pigmentosum group G (XP-G) gene (XPG) encodes a structure-specific DNA endonuclease that functions in nucleotide excision repair (NER). XP-G patients show various symptoms, ranging from mild cutaneous abnormalities to severe dermatological impairments. In some cases, exhibit growth failure and life-shortening neurological dysfunctions, which are characteristics of Cockayne syndrome (CS). known XPG protein function as the 3′ nuclease NER, however, cannot explain development CS...
Human cells contain a protein that binds to UV-irradiated DNA with high affinity. This protein, damaged DNA-binding (DDB), is heterodimer of two polypeptides, p127 and p48. Recent in vivo studies suggested DDB involved global genome repair cyclobutane pyrimidine dimers (CPDs), but the mechanism remains unclear. Here, we show vitro directly stimulates excision CPDs not (6-4)photoproducts. The activity cell-free extracts from Chinese hamster AA8 cell line lacks was increased 3-4-fold by...
Abstract To investigate UV light response mechanisms in higher plants, we isolated a light–sensitive mutant, rev3-1, Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both and dark conditions. We found that chromosome 1 broken at minimum three points, causing inversion translocation. A gene disrupted by this rearrangement encoded the catalytic subunit DNA polymerase ζ (AtREV3), which is thought to be involved translesion synthesis. seedlings also were...
Abstract— We established a monoclonal antibody(DEM–1) that recognizes UV‐induced DNA damage other than cyclobutane pyrimidine dimers or(6–4)photoproducts. The binding ofDEM–1 antibody to 254 nm UV‐irradiated increased with subsequent exposure UV wavelengths longer 310 nm, whereas of the 64M‐2 specific for the(6–4)photoproduct decreased this treatment. Furthermore, increase inDEM–1 was inhibited by presence during exposure. concluded theDEM–1 specifically recognized Dewar photoproduct, which...
Abstract Accumulating evidence indicates that transcription is closely related to DNA damage formation and the loss of RNA biogenesis factors causes genome instability. However, whether such are involved in responses remains unclear. We focus here on helicase Aquarius ( AQR ), a known R-loop processing factor, show its depletion human cells results accumulation during S phase, mediated by formation. investigated involvement found knockdown decreased damage-induced foci Rad51 replication...
Abstract UV‐induced sister chromatid exchanges (SCEs) in p53 ‐deficient mouse cells were studied to obtain more evidence regarding the involvement of protein DNA repair pathway as a checkpoint protein. After 5 J/m 2 UV irradiation, mutant‐type homozygous for ‐deficiency showed same number SCEs heterozygous and wild‐type cells. In cells, no further increase was observed after 10 irradiation. contrast, about twice many induced by UV. fractions S‐phase decreased just but recovered higher than...